Brownridge
CSF proteomics in bacterial meningitis
Emma C Wall, Philip Brownridge, Gavin Laing, Vanessa S Terra, Veronica Mlozowa, Brigitte Denis, Mulinda Nyirenda, Theresa Allain, Elisa Ramos-Sevillano, Enitan Carrol, Andrea Collins, Stephen B Gordon, David G Lalloo, Brendan Wren, Robert Beynon, Robert S Heyderman, Jeremy S Brown (2020) CSF Levels of Elongation Factor Tu Is Associated With Increased Mortality in Malawian Adults With Streptococcus pneumoniae Meningitis Front Cell Infect Microbiol doi: 10.3389/fcimb.2020.603623. eCollection 2020. [PDF][PUBMED][DOI] [Abstract...]
PhosphoLFQ of NF-kB/RelA in DNA damage
Amy E Campbell, Caterina Ferraz Franco, Ling-I Su, Emma K Corbin, Simon Perkins, Anton Kalyuzhnyy, Andrew R Jones, Philip J Brownridge, Neil D Perkins, Claire E Eyers (2021) Temporal modulation of the NF-κB RelA network in response to different types of DNA damage Biochem J doi: 10.1042/BCJ20200627. Online ahead of print. [Abstract...]
Using stable isotopes to track sperm competition
Paula Stockley, Catarina Franco, Amy J. Claydon, Amanda Davidson, Dean E. Hammond, Philip J. Brownridge, Jane L. Hurst, and Robert J. Beynon (2020) Revealing mechanisms of mating plug function under sexual selection. [PDF] [PUBMED] [DOI] [Abstract...]
Plasticity of the mouse seminal vesical fluid proteome
Helen L Bayram, Catarina Franco, Philip Brownridge, Amy J Claydon, Natalie Koch, Jane L Hurst, Robert J Beynon, Paula Stockley (2020) Social status and ejaculate composition in the house mouse [PDF] [PUBMED] [DOI] [Abstract...]
Novel proteolytic events in SARS-Cov-2 infection
Bjoern Meyer, Jeanne Chiaravalli, Philip Brownridge, Dominic P. Byrne, Leonard A. Daly, Fabrice Agou, Claire E. Eyers, Patrick A. Eyers, Marco Vignuzzi, Edward Emmott (2020) Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing [DOI] [PDF] [Abstract...]
Using QconCATs to establish protein complex stoichiometry
Mengru Yang, Deborah M Simpson, Nicolas Wenner, Philip Brownridge, Victoria M Harman, Jay C D Hinton, Robert J Beynon, Lu-Ning Liu (2020) Decoding the stoichiometric composition and organisation of bacterial metabolosomes [PDF] [PUBMED] [DOI] [Abstract...]
MS from gels, top down or bottom up
Ayako Takemori, David S Butcher, Victoria M Harman, Philip Brownridge, Keisuke Shima, Daisuke Higo, Jun Ishizaki, Hitoshi Hasegawa, Junpei Suzuki, Masakatsu Yamashita, Joseph A Loo, Rachel R Ogorzalek Loo, Robert J Beynon, Lissa C Anderson, Nobuaki Takemori (2020) PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry [PDF] [PUBMED] [DOI] [Abstract...]
Using proteomics to explore 'pseudogene' expression
Ian Goodhead, Frances Blow, Philip Brownridge, Margaret Hughes, John Kenny, Ritesh Krishna, Lynn McLean, Pisut Pongchaikul, Rob Beynon and Alistair C. Darby (202o) Large-scale and significant expression from pseudogenes in Sodalis glossinidius – a facultative bacterial endosymbiont [PDF] [PUBMED] [DOI] [Abstract...]
Using proteomics to identify targets for schistosomiasis
Leandro X Neves, R Alan Wilson, Philip Brownridge, Victoria M Harman, Stephen W Holman, Robert J Beynon, Claire E Eyers, Ricardo DeMarco, William Castro-Borges (2020) Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni Pinpoints Secreted Proteins for Vaccine Development. [PDF] [PUBMED] [DOI] [Abstract...]
SILAC Phosphoproteomics
Dominic P Byrne, Christopher J Clarke, Philip J Brownridge, Anton Kalyuzhnyy, Simon Perkins, Amy Campbell, David Mason, Andrew R Jones, Patrick A Eyers, Claire E Eyers (2020) Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics [PUBMED] [PDF] [DOI] [Abstract...]
Discovery and definition of non-canonical phosphorylation
Gemma Hardman , Simon Perkins , Philip Brownridge, Christopher J. Clarke , Dominic P. Byrne, Amy E. Campbell , Anton Kalyuzhnyy , Ashleigh Myall , Patrick A. Eyers , Andrew R. Jones and Claire E. Eyers (2019) Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation [PUBMED] [PDF] [DOI]
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Heparin binding proteome
Quentin M. Nunes, Dunhao Su, Philip J. Brownridge, Deborah M. Simpson, Changye Sun, Yong Li, Thao P. Bui, Xiaoying Zhang, Wei Huang, Daniel J. Rigden, Robert J. Beynon, Robert Sutton, David G. Fernig (2019) The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis [PUBMED] [PDF] [Abstract...]
Deep discovery of novel phosphorylation sites
Gemma Hardman, Simon Perkins, Zheng Ruan, Natarajan Kannan, Philip Brownridge, Dominic P Byrne, Patrick A Eyers, Andrew R Jones (2017) Extensive non-canonical phosphorylation in human cells revealed using strong-anion exchange-mediated phosphoproteomics [PDF] [BIORXIV] [Abstract...]
Enhanced ability in phosphosite localisation
Ferries S, Perkins S, Brownridge PJ, Campbell A, Eyers PA, Jones AR, Eyers CE. (2017) Evaluation of Parameters for Confident Phosphorylation Site Localization Using an Orbitrap Fusion Tribrid Mass Spectrometer. [PDF] [PUBMED] [Abstract...]
Double standards in proteomics
Richard J. Bennett, Deborah M. Simpson, Stephen W. Holman, Sheila Ryan, Philip Brownridge, Claire E. Eyers, John Colyer, Robert J. Beynon (2017) DOSCATs: Double standards for protein quantification [PDF] [PUBMED] [Abstract...]
The joys of running a training course!
A short article, written for the Biochemist, explaining the role of the Society in training provision and our experiences of running the Quantitative Proteomics Course [PDF] [Abstract...]
The biggest absolute quantification study ever! A tale of 100 QconCATs for nearly 2,000 proteins
Lawless C, Holman SW, Brownridge P, Lanthaler K, Harman VM, Watkins R, Hammond DE, Miller RL, Sims PF, Grant CM, Eyers CE, Beynon RJ, Hubbard SJ. (2016) Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring. [Abstract...]
Sperm proteomes, gone large
Bayram HL, Claydon AJ, Brownridge PJ, Hurst JL, Mileham A, Stockley P, Beynon RJ, Hammond DE.(2016) Cross-species proteomics in analysis of mammalian sperm proteins. [PUBMED] [PDF] [Abstract...]
Of mice, mups and mass spectrometry
Robert J. Beynon, Stuart D. Armstrong, Amy J. Claydon, Amanda J. Davidson, Claire E. Eyers, James I. Langridge, Guadalupe Gómez-Baena, Victoria M. Harman, Jane L. Hurst, Victoria Lee, Lynn McLean, Rebecca Pattison, Sarah A. Roberts, Deborah M. Simpson, Jenny Unsworth, Matthias Vonderach, Jonathan Williams and Yvonne E. Woolerton (2015) Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse. [PDF] [NotEvenIndexedInPubmed?] [Abstract...]
Proteome plasticity in sperm competition
Ramm SA, Edward DA, Claydon AJ, Hammond DE, Brownridge, Hurst JL, Beynon RJ, Stockley P. (2015) Sperm competition risk drives plasticity in seminal fluid composition. [PUBMED] [PDF] [Abstract...]
QconCAT for equine acute phase proteins
Bundgaard L, Jacobsen S, Dyrlund TF, Sørensen MA, Harman VM, Beynon RJ, Brownridge PJ, Petersen LJ, Bendixen E. (2014) Development of a method for absolute quantification of equine acute phase proteins using concatenated peptide standards and selected reaction monitoring. [PUBMED][PDF]
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Who's got the biggest workload? Analysis of chaperone abundance and workload
Brownridge P, Lawless C, Payapilly AB, Lanthaler K, Holman SW, Harman VM, Grant CM, Beynon RJ, Hubbard SJ. Quantitative analysis of chaperone network throughput in budding yeast (2012). [PUBMED][PDF] [Abstract...]
Who's got the biggest workload? Analysis of chaperone abundance and workload
Brownridge P, Lawless C, Payapilly AB, Lanthaler K, Holman SW, Harman VM, Grant CM, Beynon RJ, Hubbard SJ. Quantitative analysis of chaperone network throughput in budding yeast (2012). [PUBMED][PDF] [Abstract...]
Extending Progenesis to embrace SILAC and Hi3
Qi, D., Brownridge, P., Xia, D., Mackay, K., Gonzalez-Galarza, F.F., Kenyani, J., Harman, V., Beynon, R.J. & Jones, A.R. (2012) A software toolkit and interface for performing stable isotope labelling and top3 quantification using Progenesis LC-MS OMICS [PUBMED] [PDF] [Abstract...]
Quantification of a metabolic pathway
Carroll KM, Simpson DM, Eyers CE, Knight CG, Brownridge P, Dunn W, Winder CL, Lanthaler K, Pir P, Malys N, Kell DB, Oliver SG, Gaskell SJ, Beynon RJ. (2011) Absolute quantification of a metabolic pathway in yeast: deployment of a complete QconCAT approach. [PUBMED][PDF] [Abstract...]
Unkind cuts - proteolysis and proteomics
Brownridge, P. & Beynon, R.J. (2011) The importance of the digest: proteolysis and absolute quantification in proteomics. [PUBMED] [PDF] [Abstract...]
Global proteome quantification: dreams or reality?
Brownridge, P., Holman, S.W., Gaskell, S.J., Grant, C., Harman, V.M., Lanthaler, K., Lawless, C., O’cualain, R., Sims, P., Watkins, R & Beynon, R.J. (2011) Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy. Proteomics, 11, 2957-2970 [PUBMED][PDF] [Abstract...]