Signalling
SILAC Phosphoproteomics
Dominic P Byrne, Christopher J Clarke, Philip J Brownridge, Anton Kalyuzhnyy, Simon Perkins, Amy Campbell, David Mason, Andrew R Jones, Patrick A Eyers, Claire E Eyers (2020) Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics [PUBMED] [PDF] [DOI] [Abstract...]
Regulation of Aurora A by CoASH
Yugo Tsuchiya, Dominic P Byrne, Selena G Burgess, Jenny Bormann, Jovana Baković, Yueyang Huang, Alexander Zhyvoloup, Bess Yi Kun Yu, Sew Peak-Chew, Trang Tran, Fiona Bellany, Alethea B Tabor, Aw Edith Chan, Lalitha Guruprasad, Oleg Garifulin, Valeriy Filonenko, Matthias Vonderach, Samantha Ferries, Claire E Eyers, John Carroll, Mark Skehel, Richard Bayliss , Patrick A Eyers, Ivan Gout (2020) Covalent Aurora A regulation by the metabolic integrator coenzyme A. [PDF] [PUBMED] [DOI] [Abstract...]
Stoichiometry of histidine kinases by mass spectrometry
Lauren J Tomlinson, Alice K M Clubbs Coldron , Patrick A Eyers, Claire E Eyers (2020) Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry. [PDF] [PUBMED] [DOI] [Abstract...]
Methodology for hunting histidine phosphorylation
Gemma Hardman and Claire E. Eyers (2020) High-Throughput Characterization of Histidine Phosphorylation Sites Using UPAX and Tandem Mass Spectrometry. [PDF] [PUBMED] [DOI] [Abstract...]
Redox control of protein kinases
Dominic P Byrne, Safal Shrestha , Martin Galler, Min Cao, Leonard A Daly, Amy E Campbell, Claire E Eyers, Elizabeth A Veal, Natarajan Kannan Patrick A Eyers (2020) Aurora A regulation by reversible cysteine oxidation reveals evolutionarily conserved redox control of Ser/Thr protein kinase activity. [PDF] [PUBMED] [DOI] [Abstract...]
Protein sulfation enzymes are targeted by kinase inhibitors
Byrne DP, Li Y1, Ngamlert P, Ramakrishnan K, Eyers CE, Wells C, Drewry DH, Zuercher WJ, Berry NG, Fernig DG, Eyers PA. (2018) New tools for evaluating protein tyrosine sulfation: tyrosylprotein sulfotransferases (TPSTs) are novel targets for RAF protein kinase inhibitors. [PUBMED] [PDF] [Abstract...]
Enzymes for carbohydrate sulfation are targeted by kinase inhibitors
Dominic P. Byrne, Yong Li, Krithika Ramakrishnan, Igor L. Barsukov, Edwin A. Yates, Claire E. Eyers, Dulcé Papy-Garcia, Sandrine Chantepie, Vijayakanth Pagadala, Jian Liu, Carrow Wells, David H. Drewry, William J. Zuercher, Neil G. Berry, David G. Fernig and Patrick A. Eyers (2018) New tools for carbohydrate sulfation analysis: heparan sulfate 2-O-sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors [PDF] [PUBMED] [Abstract...]
Role of CDK18 in replication stress signalling and genome stability
Barone G, Staples CJ, Ganesh A, Patterson KW, Bryne DP, Myers KN, Patil AA, Eyers CE, Maslen S, Skehel JM, Eyers PA, Collis SJ. (2016). Human CDK18 promotes replication stress signaling and genome stability. [PUBMED] [PDF] [Abstract...]