Dynamic SILAC
A degrading synthesis of proteome turnover research!
Claydon, A.C. & Beynon, R.J. (2012) Proteome dynamics: revisiting turnover with a global perspective. Mol. Cell Proteomics 11:1551-1565. [PUBMED][PDF] [Abstract...]
Dynamic sperm labelling
Claydon AJ, Ramm SA, Pennington A, Hurst JL, Stockley P, Beynon R. (2012) Heterogenous turnover of sperm and seminal vesicle proteins in the mouse revealed by dynamic metabolic labeling. Mol Cell Proteomics. 11(6):M111.014993. [PUBMED][PDF] [Abstract...]
Whole animal protein turnover studies
Claydon, A.J., Thom, M.D., Hurst, J.L. & Beynon, R.J. (2011) Protein turnover: measurement of proteome dynamics by whole animal metabolic labelling with stable isotope labelled amino acids. Proteomics 12:1194-1206 [PUBMED][PDF] [Abstract...]
Enzyme turnover and metabolic regulation
Arden, C, Petrie, J.L., Tudhope, S.J., Al-Oanzi, Z., Claydon, A.J., Beynon, R.J., Towle, H.C. & Agius, L. (2011) Diabetes Journal (in press) Elevated glucose represses liver glucokinase and induces its regulatory protein to safeguard hepatic phosphate homeostasis [PUBMED][PDF] [Abstract...]
Strategies for dynamic SILAC
Claydon, A & Beynon R (2011) Methods Mol Biol. 759, 179-195. Protein Turnover Methods in Single-Celled Organisms: Dynamic SILAC [Abstract...]
Dynamic SILAC for global assessment of proteome turnover
Doherty, M.K., Hammond, D., Clague, M. J., Gaskell, S.J. & Beynon, R.J. (2009) Turnover of the human proteome: determination of protein intracellular stability by dynamic SILAC. J. Proteome Res. 8, 104-112 [PUBMED] [PDF] [Abstract...]
Proteome dynamics
Doherty MK, Beynon RJ. (2006) Protein turnover on the scale of the proteome. Expert Rev Proteomics. 1, 97-110[PUBMED] [PDF] [Abstract...]
Proteome turnover: why is it not being studied?
Beynon, R.J. & Pratt, J.M. (2006) Strategies for measuring dynamics: the temporal component of proteomics. Methods Biochem Anal. 2006;49:15-25 [PUBMED] [Abstract...]
Metabolic labelling in proteomics
Beynon RJ, Pratt J.M. (2005) Metabolic labelling of proteins for proteomics. Mol Cell Proteomics. 4, 857-872 [PUBMED] [PDF] [Abstract...]
Proteome dynamics review
Beynon RJ.(2005) The dynamics of the proteome: strategies for measuring protein turnover on a proteome-wide scale. Brief Funct Genomic Proteomic. 3, 382-390 [PUBMED] [PDF] [Abstract...]
First paper on dynamic SILAC in intact animals
Doherty, M.K., Whitehead, C., McCormack, H., Gaskell, S.J. & Beynon, R.J. (2005) Proteome dynamics in complex organisms: using stable isotopes to monitor individual protein turnover rates. Proteomics 5, 522-533 [PUBMED] [PDF] [Abstract...]
Protein turnover in a vertebrate
Doherty MK, McClean L, Edwards I, McCormack H, McTeir L, Whitehead C, Gaskell SJ, Beynon RJ. (2004) Protein turnover in chicken skeletal muscle: understanding protein dynamics on a proteome-wide scale. Br Poult Sci. 45 Suppl 1:S27-8 [PUBMED] [PDF] [Abstract...]
First paper on proteome turnover
Pratt, J. M., Petty, J., Riba-Garcia, I., Robertson, D. H., Gaskell, S. J., Oliver, S. G. & Beynon, R. J. (2002). Dynamics of protein turnover, a missing dimension in proteomics. Mol Cell Proteomics 1, 579-91. [PUBMED] [PDF] [Abstract...]