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IDENTIFICATION AND CLONING OF HUMAN P-2U PURINOCEPTOR PRESENT
IN OSTEOCLASTOMA, BONE, AND OSTEOBLASTS BOWLER_WB, BIRCH_MA, GALLAGHER_JA, BILBE_G JOURNAL OF BONE AND MINERAL RESEARCH, 10, 1137-1145 Extracellular ATP acting through purinoceptors may be an important factor in the modulation of bone turnover, In this study we cloned and sequenced the P-2U purinoceptor from osteoclastoma, confirming the recently published human sequence. Furthermore, by the reverse transcriptase-linked polymerase chain reaction (RT-PCR) and Southern blotting we demonstrated expression of P-2U receptor mRNA in bone, primary cultures of human bone-derived cells, and two osteosarcoma cell lines, Saos2 and Te85. P-2U receptor transcripts were identified in alkaline phosphatase-positive human bone-derived cells isolated by now cytometry providing strong evidence for the expression of the P-2U purinoceptor in mature osteoblasts. P-2U receptor transcripts were also detected in a purified giant cell population isolated hom osteoclastoma, indicating that this receptor is also expressed by osteoclasts. These data suggest that purinergic agonists may play a role in the regulation of bone metabolism. |