Research outputs
Selected research outputs
- Community-developed checklists for publishing images and image analyses (Journal article - 2023)
- Pulmonary SARS-CoV-2 infection leads to para-infectious immune activation in the brain (Journal article - 2024)
- Quantification of fluorescence intensity of antisense constructs within Bodo saltans and its intracellular symbiont (Other - 2024)
- Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy (Journal article - 2018)
- Seven Steps to FAIRness Illustrations by Marie Held (Digital or visual media - 2026)
2026
Lysosomal signaling pathways influence heart rhythm, and regulate atrial function.
Akerman, E., Capel, R. A., Rog-Zielinska, E. A., Winbo, A., Aston, D., Falter, F., . . . Burton, R. A. B. (2026). Lysosomal signaling pathways influence heart rhythm, and regulate atrial function.. Cardiovasc Res. doi:10.1093/cvr/cvag207
Bloodstream-associated Salmonella Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages
Amadeo, F., Held, M., Pennington, S. H., Biagini, G. A., Hinton, J. C. D., & Cattelan, N. (2026). Bloodstream-associated Salmonella Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages. Wellcome Open Research, 11, 673. doi:10.12688/wellcomeopenres.27234.1
Establishing a Microfluidic Co-Culture Blood-Brain Barrier Model Using Human Brain Endothelial Cells and Astrocytes.
Boardman, S. A., Rosano, J. M., Hetherington, C. D., Dunai, C., Bateman-Price, T. D., Held, M., . . . Michael, B. D. (2026). Establishing a Microfluidic Co-Culture Blood-Brain Barrier Model Using Human Brain Endothelial Cells and Astrocytes.. Journal of visualized experiments : JoVE, (235). doi:10.3791/71652
Biochemistry Focus webinar – FAIR by Design: Practical Strategies for Reproducible Microscopy
Held, M., Awais, R., Lancaster, L., Awais, M., & Society, B. (n.d.). Biochemistry Focus webinar – FAIR by Design: Practical Strategies for Reproducible Microscopy. [Webinar video recording]. Retrieved from https://www.youtube.com/watch?v=W6JQ1cJYr6A
Github account
Held, M. (n.d.). Github account [Computer Software]. Retrieved from https://github.com/Marien-kaefer
Seven Steps to FAIRness Illustrations by Marie Held
Held, M. (n.d.). Seven Steps to FAIRness Illustrations by Marie Held. doi:10.5281/zenodo.20854554
Building capacity in imaging data management
Velasquez, S. M., Fletcher, G., Burel, J. -M., Parsons, M., Swedlow, J., Hartley, M., . . . Yoldas, A. (2026). Building capacity in imaging data management. JOURNAL OF MICROSCOPY, 302(3), 334-345. doi:10.1111/jmi.70078
Topical Formulation of Repurposed FDA-Approved Compounds Inhibits Pseudomonas aeruginosa ExoU and Improves Corneal Infection Outcome
Foulkes, D. M., Fernig, D. G., McLean, K., Held, M., Harris, J., Sun, Y., . . . Kaye, S. B. (n.d.). Topical Formulation of Repurposed FDA-Approved Compounds Inhibits Pseudomonas aeruginosa ExoU and Improves Corneal Infection Outcome. doi:10.6019/s-biad2625
REWRITING THE MESSAGE: INFLAMMATION-DRIVEN EXTRACELLULAR VESICLE REPROGRAMMING PROVIDES NEW MECHANISTIC INSIGHTS FOR STEM-CELL BASED OSTEOARTHRITIS THERAPIES
Clarke, E., Poulter, N., Becket, A., Dykes, G., Bakker, S., Jensen, A., . . . Peffers, M. (2026). REWRITING THE MESSAGE: INFLAMMATION-DRIVEN EXTRACELLULAR VESICLE REPROGRAMMING PROVIDES NEW MECHANISTIC INSIGHTS FOR STEM-CELL BASED OSTEOARTHRITIS THERAPIES. OSTEOARTHRITIS AND CARTILAGE, 34, S312. Retrieved from https://www.webofscience.com/
Assembly and Functional Coordination of Two Families of Metabolic Organelles in Salmonella
Chang, P., Yang, M., Chen, Y., Li, T., Held, M., & Liu, L. -N. (2026). Assembly and Functional Coordination of Two Families of Metabolic Organelles in Salmonella. MICROBIAL BIOTECHNOLOGY, 19(2), 15 pages. doi:10.1111/1751-7915.70301
Bloodstream-associated <i>Salmonella</i> Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages
Topical formulation of repurposed FDA-approved compounds inhibits <i>Pseudomonas aeruginosa</i> ExoU and improves corneal infection outcomes
Foulkes, D. M., Fernig, D. G., McLean, K., Held, M., Harris, J. A., Sun, Y., . . . Kaye, S. B. (2026). Topical formulation of repurposed FDA-approved compounds inhibits <i>Pseudomonas aeruginosa</i> ExoU and improves corneal infection outcomes. PLOS PATHOGENS, 22(8), 36 pages. doi:10.1371/journal.ppat.1014474
2025
Towards developing inhibitors of the Pseudomonas aeruginosa exotoxin U (ExoU) with high throughput imaging and analysis
Held, M., Foulkes, D., McLean, K., Waring, T., Kaye, S., & Fernig, D. (2025). Towards developing inhibitors of the Pseudomonas aeruginosa exotoxin U (ExoU) with high throughput imaging and analysis. Poster session presented at the meeting of CBIAS 2025. Francis Crick Institute, London, UK. doi:10.25418/crick.30869549
Topical Formulation of Repurposed FDA-Approved Compounds Inhibits <i>Pseudomonas aeruginosa</i> ExoU and Improves Corneal Infection Outcomes
Molecular basis of the biogenesis of a protein organelle for ethanolamine utilization
Yang, M., Adegbite, O., Chang, P., Cheng, J., Wang, Y., Held, M., . . . Liu, L. -N. (2025). Molecular basis of the biogenesis of a protein organelle for ethanolamine utilization. SCIENCE ADVANCES, 11(40), 17 pages. doi:10.1126/sciadv.adx9774
Hierarchical Structure of the Program Used by Filamentous Fungi to Navigate in Confining Microenvironments
Montiel-Rubies, G., Held, M., Hanson, K. L., Nicolau, D. V., Mocanasu, R. C., van Delft, F. C. M. J. M., & Nicolau, D. V. (2025). Hierarchical Structure of the Program Used by Filamentous Fungi to Navigate in Confining Microenvironments. BIOMIMETICS, 10(5), 25 pages. doi:10.3390/biomimetics10050287
Development of Antisense Tools to Study <i>Bodo saltans</i> and Its Intracellular Symbiont
Ahrar, M., Glenn, L., Held, M., Jackson, A., Kus, K., Hurst, G. D. D., & Chrostek, E. (2025). Development of Antisense Tools to Study <i>Bodo saltans</i> and Its Intracellular Symbiont. MICROBIOLOGYOPEN, 14(2), 9 pages. doi:10.1002/mbo3.70018
Raw imaging data for Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy
Held, M., Santeramo, I., Wilm, B., Murray, P., & Levy, R. (n.d.). Raw imaging data for Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. doi:10.17867/10000110
Arrhythmogenic calmodulin variants D131E and Q135P disrupt interaction with the L-type voltage-gated Ca2+ channel (Cav1.2) and reduce Ca2+-dependent inactivation.
Gupta, N., Richards, E. M. B., Morris, V. S., Morris, R., Wadmore, K., Held, M., . . . Helassa, N. (2025). Arrhythmogenic calmodulin variants D131E and Q135P disrupt interaction with the L-type voltage-gated Ca2+ channel (Cav1.2) and reduce Ca2+-dependent inactivation.. Acta physiologica (Oxford, England), 241(2), e14276. doi:10.1111/apha.14276
2024
Pulmonary SARS-CoV-2 infection leads to para-infectious immune activation in the brain
Dunai, C., Hetherington, C., Boardman, S. A., Clark, J. J., Sharma, P., Subramaniam, K., . . . Michael, B. D. (2024). Pulmonary SARS-CoV-2 infection leads to para-infectious immune activation in the brain. Frontiers in Immunology, 15. doi:10.3389/fimmu.2024.1440324
Quantification of fluorescence intensity of antisense constructs within Bodo saltans and its intracellular symbiont
Held, M., Ahrar, M., Hurst, G., & Chrostek, E. (2024). Quantification of fluorescence intensity of antisense constructs within Bodo saltans and its intracellular symbiont.
Did you know – Image Analysis Style
Held, M. (2024). Did you know – Image Analysis Style. Retrieved from https://didyouknowimageanalysis.wordpress.com/
Para-infectious brain injury in COVID-19 persists at follow-up despite attenuated cytokine and autoantibody responses (vol 14, 8487, 2023)
Michael, B. D., Dunai, C., Needham, E. J., Tharmaratnam, K., Williams, R., Huang, Y., . . . Menon, D. K. (2024). Para-infectious brain injury in COVID-19 persists at follow-up despite attenuated cytokine and autoantibody responses (vol 14, 8487, 2023). NATURE COMMUNICATIONS, 15(1), 1 page. doi:10.1038/s41467-024-47320-6
2023
Outcome Of A Cohort Of Older Population With COPD, Admitted With Hypercapnaeic Respiratory Failure And Acidosis
Dushianthan, A., Temblett, P., & Bhatta, A. (2010). Outcome Of A Cohort Of Older Population With COPD, Admitted With Hypercapnaeic Respiratory Failure And Acidosis. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 181, 1 page. Retrieved from https://www.webofscience.com/
Community-developed checklists for publishing images and image analyses
Schmied, C., Nelson, M. S., Avilov, S., Bakker, G. -J., Bertocchi, C., Bischof, J., . . . Jambor, H. K. (2023). Community-developed checklists for publishing images and image analyses. Nature Methods. doi:10.1038/s41592-023-01987-9
Community-developed checklists for publishing images and image analysis
Long QT syndrome-associated calmodulin variants disrupt the activity of the slow delayed rectifier potassium current
McCormick, L., Wadmore, K., Milburn, A., Gupta, N., Morris, R., Held, M., . . . Helassa, N. (2023). Long QT syndrome-associated calmodulin variants disrupt the activity of the slow delayed rectifier potassium current. JOURNAL OF PHYSIOLOGY-LONDON, 601(17), 3739-3764. doi:10.1113/JP284994
Did you know – Image Analysis Style
Held, M. (2023). Did you know – Image Analysis Style. Retrieved from https://didyouknowimageanalysis.wordpress.com/
2022
CPVT-associated calmodulin variants N53I and A102V dysregulate calcium signalling via different mechanisms
Prakash, O., Held, M., McCormick, L. F., Gupta, N., Lian, L. -Y., Antonyuk, S., . . . Helassa, N. (2022). CPVT-associated calmodulin variants N531 and A102V dysregulate Ca<SUP>2+</SUP> signalling via different mechanisms. JOURNAL OF CELL SCIENCE, 135(2), 15 pages. doi:10.1242/jcs.258796
2019
The Centre for Cell Imaging at the University of Liverpool
Held, M., Marcello, M., Waring, T., & Zech, T. (2019). The Centre for Cell Imaging at the University of Liverpool. Retrieved from https://cci.liv.ac.uk/index.html
Intracellular mechanisms of fungal space searching in microenvironments.
Held, M., Kašpar, O., Edwards, C., & Nicolau, D. V. (2019). Intracellular mechanisms of fungal space searching in microenvironments.. Proceedings of the National Academy of Sciences of the United States of America. doi:10.1073/pnas.1816423116
2018
Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells <i>in Vivo</i> (vol 10, pg 7106, 2016)
Comenge, J., Fragueiro, O., Sharkey, J., Taylor, A., Held, M., Burton, N. C., . . . Levy, R. (2018). Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells <i>in Vivo</i> (vol 10, pg 7106, 2016). ACS NANO, 12(8), 8831. doi:10.1021/acsnano.8b05577
Intracellular mechanisms of fungal space searching in microenvironments
Held, M., Kaspar, O., Edwards, C., & Nicolau, D. (2018). Intracellular mechanisms of fungal space searching in microenvironments. doi:10.1101/391797
Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy
Held, M., Santeramo, I., Wilm, B., Murray, P., & Levy, R. (2018). Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. PLoS One, 13(7). doi:10.1371/journal.pone.0199918
Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy
Held, M., Santeramo, I., Wilm, B., Murray, P., & Lévy, R. (2018). Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. PLoS One. doi:10.1371/journal.pone.0199918
2017
Cellular crosstalk between airway epithelial and endothelial cells regulates barrier functions during exposure to double-stranded RNA
Blume, C., Reale, R., Held, M., Loxham, M., Millar, T. M., Collins, J. E., . . . Davies, D. E. (2017). Cellular crosstalk between airway epithelial and endothelial cells regulates barrier functions during exposure to double-stranded RNA. IMMUNITY INFLAMMATION AND DISEASE, 5(1), 45-56. doi:10.1002/iid3.139
2016
Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells in Vivo
Comenge, J., Fragueiro, O., Sharkey, J., Taylor, A., Held, M., Burton, N. C., . . . Lévy, R. (2016). Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells in Vivo. ACS Nano, 10(7), 7106-7116. doi:10.1021/acsnano.6b03246
Dispersion of Hydrophobic Co Supracrystal in Aqueous Solution
Yang, N., Yang, Z., Held, M., Bonville, P., Albouy, P. -A., Levy, R., & Pileni, M. -P. (2016). Dispersion of Hydrophobic Co Supracrystal in Aqueous Solution. ACS NANO, 10(2), 2277-2286. doi:10.1021/acsnano.5b06966
2015
Temporal Monitoring of Differentiated Human Airway Epithelial Cells Using Microfluidics
Blume, C., Reale, R., Held, M., Millar, T. M., Collins, J. E., Davies, D. E., . . . Swindle, E. J. (2015). Temporal Monitoring of Differentiated Human Airway Epithelial Cells Using Microfluidics. PLOS ONE, 10(10), 13 pages. doi:10.1371/journal.pone.0139872
Localisation and properties of nanoparticle conjugates after cell entry ... and why it matters
Mason, D., Comenge, J., Carolan, G., Levy, R., & Held, M. (2015). Localisation and properties of nanoparticle conjugates after cell entry ... and why it matters. In ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY Vol. 250 (pp. 2 pages). Retrieved from https://www.webofscience.com/
The Spherical Nucleic Acids mRNA Detection Paradox
Levy, R., Held, M., Mason, D., Comenge, J., Carolan, G., & Cowman, S. (2016). The Spherical Nucleic Acids mRNA Detection Paradox. ScienceOpen Research, 0(0). doi:10.14293/s2199-1006.1.sor-chem.az1mju.v2
The Spherical Nucleic Acids mRNA Detection Paradox
Mason, D., Carolan, G., Held, M., Comenge, J., & Lévy, R. (2016). The Spherical Nucleic Acids mRNA Detection Paradox. ScienceOpen Research, 0(0). doi:10.14293/s2199-1006.1.sor-chem.az1mju.v1
2011
Probing the growth dynamics of <i>Neurospora crassa</i> with microfluidic structures
Held, M., Edwards, C., & Nicolau, D. V. (2011). Probing the growth dynamics of <i>Neurospora crassa</i> with microfluidic structures. FUNGAL BIOLOGY, 115(6), 493-505. doi:10.1016/j.funbio.2011.02.003
Hyphal responses of <i>Neurospora crassa</i> to micron-sized beads with functional chemical surface groups
Held, M., Edwards, C., & Nicolau, D. V. (2011). Hyphal responses of <i>Neurospora crassa</i> to micron-sized beads with functional chemical surface groups. In IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX Vol. 7902 (pp. 9 pages). doi:10.1117/12.881721
Hyphal responses of Neurospora crassa to micron-sized beads with functional chemical surface groups
Held, M., Edwards, C., & Nicolau, D. V. (2011). Hyphal responses of Neurospora crassa to micron-sized beads with functional chemical surface groups. doi:10.1117/12.881721
2010
Temporal and spatial <i>in vivo</i> optical analysis of microtubules in <i>Neurospora crassa</i>
Held, M., Edwards, C., & Nicolau, D. V. (2010). Temporal and spatial <i>in vivo</i> optical analysis of microtubules in <i>Neurospora crassa</i>. In IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES VIII Vol. 7568 (pp. 7 pages). doi:10.1117/12.841328
Microfluidics structures for probing the dynamic behaviour of filamentous fungi
Held, M., Lee, A. P., Edwards, C., & Nicolau, D. V. (2010). Microfluidics structures for probing the dynamic behaviour of filamentous fungi. MICROELECTRONIC ENGINEERING, 87(5-8), 786-789. doi:10.1016/j.mee.2009.11.096
Temporal and spatial in vivo optical analysis of microtubules in Neurospora crassa
Held, M., Edwards, C., & Nicolau, D. V. (2010). Temporal and spatial in vivo optical analysis of microtubules in Neurospora crassa. SPIE Proceedings, 7568, 75680V. doi:10.1117/12.841328
2009
Fungal intelligence; Or on the behaviour of microorganisms in confined micro-environments
Held, M., Edwards, C., & Nicolau, D. V. (2009). Fungal intelligence; Or on the behaviour of microorganisms in confined micro-environments. Journal of Physics: Conference Series, 178, 012005. doi:10.1088/1742-6596/178/1/012005
A study on the atomic hydrophobicity of peptides in aqueous solutions using molecular dynamics modeling methods
Nguyen, M. T., Chaffee, A. L., Held, M., Boysen, R. I., Nicolau, D. V., & Hearn, M. T. W. (2008). A study on the atomic hydrophobicity of peptides in aqueous solutions using molecular dynamics modeling methods. In BIOMEDICAL APPLICATIONS OF MICRO- AND NANOENGINEERING IV AND COMPLEX SYSTEMS Vol. 7270 (pp. 10 pages). doi:10.1117/12.810715
Dynamic Behaviour of Fungi in Microfluidics - A comparative study
Held, M., Binz, M., Edwards, C., & Nicolau, D. V. (2009). Dynamic Behaviour of Fungi in Microfluidics - A comparative study. In IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES VII Vol. 7182 (pp. 9 pages). doi:10.1117/12.822464
Dynamic behaviour of microorganisms on microstructures
Held, M., Komaromy, A., Fulga, F., Edwards, C., Boysen, R. I., Hearn, M. T. W., & Nicolau, D. V. (2009). Dynamic behaviour of microorganisms on microstructures. MICROELECTRONIC ENGINEERING, 86(4-6), 1455-1458. doi:10.1016/j.mee.2009.01.012
Dynamic behaviour of fungi in microfluidics: a comparative study
Held, M., Binz, M., Edwards, C., & Nicolau, D. V. (2009). Dynamic behaviour of fungi in microfluidics: a comparative study. SPIE Proceedings, 7182, 718213. doi:10.1117/12.822464
Fungal Intelligence; Or on the behaviour of microorganisms in confined micro-environments
Held, M., Edwards, C., & Nicolau, D. V. (2009). Fungal Intelligence; Or on the behaviour of microorganisms in confined micro-environments. SENSORS & THEIR APPLICATIONS XV, 178, 6 pages. doi:10.1088/1742-6596/178/1/012005
2008
Examining the behaviour of fungal cells in microconfined mazelike structures
Held, M., Edwards, C., & Nicolau, D. V. (2008). Examining the behaviour of fungal cells in microconfined mazelike structures. In IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES VI Vol. 6859 (pp. 9 pages). doi:10.1117/12.759453
Estimation of atomic hydrophobicities using Molecular Dynamics simulation of peptides
Held, M., & Nicolau, D. V. (2008). Estimation of atomic hydrophobicities using Molecular Dynamics simulation of peptides. In BIOMEMS AND NANOTECHNOLOGY III Vol. 6799 (pp. 7 pages). doi:10.1117/12.759385
Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia: A same difference diagnosis for patients mit multiple pulmonary round lesion
Schedelbeck, U., Held, M., Geissinger, E., & Marten, K. (2008). Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia: A same difference diagnosis for patients mit multiple pulmonary round lesion. ROFO-FORTSCHRITTE AUF DEM GEBIET DER RONTGENSTRAHLEN UND DER BILDGEBENDEN VERFAHREN, 180(3), 257-259. doi:10.1055/s-2008-102701
Examining the behaviour of fungal cells in microconfined mazelike structures
Held, M., Edwards, C., & Nicolau, D. V. (2008). Examining the behaviour of fungal cells in microconfined mazelike structures. SPIE Proceedings, 6859, 68590U. doi:10.1117/12.759453
2007
Estimation of atomic hydrophobicities using molecular dynamics simulation of peptides
Held, M., & Nicolau, D. V. (2007). Estimation of atomic hydrophobicities using molecular dynamics simulation of peptides. SPIE Proceedings, 6799, 679916. doi:10.1117/12.759385
Formation of Au/Ag nanoparticles in a two step micro flow-through process
Koehler, J. M., Held, M., Huebner, U., & Wagner, J. (2007). Formation of Au/Ag nanoparticles in a two step micro flow-through process. CHEMICAL ENGINEERING & TECHNOLOGY, 30(3), 347-354. doi:10.1002/ceat.200600388
2006
Formation of Au/Ag nanoparticles in a two step micro flow through process
Köhler, J. M., Held, M., Hübner, U., & Wagner, J. (2006). Formation of Au/Ag nanoparticles in a two step micro flow through process. In Aiche Annual Meeting Conference Proceedings.
Formation of Au/Ag nanoparticles in a two step micro flow through process
Köhler, J. M., Held, M., Hübner, U., & Wagner, J. (2006). Formation of Au/Ag nanoparticles in a two step micro flow through process. In Topical Conference on Applications of Micro Reactor Engineering 2006 Held at the 2006 Aiche Spring National Meeting (pp. 215-226).