BIOL411 - Genome Maintenance and Rearrangement

CONTENTS | NEXT PAGE

BIOL411 - Genome Maintenance and Rearrangement

Module co-ordinator : Dr. M.X. Caddick

Location

Science Lecture Block LTD/C

Times

Monday (LTD), Tuesday (LTC): 16.00

Week
Lecture Content

13
2
Dr. W. Greenhalf

Chromosome structure and cell cycle.

Telomeres, centromeres and heterochromatin.

The cell cycle and its regulation

14-15
4
Dr. N.J. Jones

DNA repair in prokaryotes and eukaryotes

Survival curves - theoretical considerations: isolation of UVS mutants: properties of uvr mutants: purification of uvr proteins and their characteristics: models of excision repair. Isolation and characterisation of rec-mutants: post-replication repair: recA and lexA mutants - phenotypic characterisation. SOS response - number of genes: LexA binding sites: differential control of expression: cleavage of LexA by RecA. The adaptive response: repair of alkylation damage: glycosylases and their activities: methyltransferases: genetics of the adaptive response - positive control by the Ada protein. Mismatch repair - genetic evidence: genes involved: in vitro systems, role of dam methylase; heat shock response and other inducible systems. Isolation and characterisation of mutants in model fungal and mammalian systems: DNA repair deficiencies associated with heritable diseases (XP, AT etc.): cloning of DNA repair genes: enzymology of eukaryotic DNA repair.

16-18
6
Prof. P. Strike

Recombination in prokaryotes and eukaryotes

Generalised recombination in E.coli: Holliday model: in vivo evidence for Holliday intermediates. Isolation of rec- mutants: properties of recA, recB recC, recF, recN, sbcA and sbcB: properties of exonuclease V and III: models of recombination pathways. D-loop formation: in vivo properties of RecA protein: strand exchange and transfer: in vivo generation of Holliday structures. The genetics of recombination in yeast: Early stages in meiosis, RecA-like and other recombination proteins from eukaryotes.

Transposition in prokaryotes

Insertion sequences and compound transposons. The Tn3 family; enzymology of transposition. Bacteriophage

19-21
6
Professor Jon Saunders

Invertible control systems

Hin flagellar phase variation. Pin/Gin/Cin invertases. Molecular basis of antigenic variability in pathogenic bacteria. Slipped-strand mispairing modulation of bacterial virulence.

Genetics and Molecular Biology of Plasmids.

Physiology and genetics of bacterial conjugation; trd operon and fertility control.

Role of Trd proteins in mating and DNA metabolism. Mobilisation of non-conjugative plasmids. Plasmid structure and function, micro- and macro-evolution. Plasmid replication in relation to cell cycle, amplification, control of copy number and incompatibility. Non-coding RNA and protein controls.

CONTENTS | NEXT PAGE

Location	Science Lecture Block LTD/C
Times	Monday (LTD), Tuesday (LTC): 16.00

Week	Lecture	Content
13	2	Dr. W. Greenhalf Chromosome structure and cell cycle. Telomeres, centromeres and heterochromatin. The cell cycle and its regulation
14-15	4	Dr. N.J. Jones DNA repair in prokaryotes and eukaryotes Survival curves - theoretical considerations: isolation of UVS mutants: properties of uvr mutants: purification of uvr proteins and their characteristics: models of excision repair. Isolation and characterisation of rec-mutants: post-replication repair: recA and lexA mutants - phenotypic characterisation. SOS response - number of genes: LexA binding sites: differential control of expression: cleavage of LexA by RecA. The adaptive response: repair of alkylation damage: glycosylases and their activities: methyltransferases: genetics of the adaptive response - positive control by the Ada protein. Mismatch repair - genetic evidence: genes involved: in vitro systems, role of dam methylase; heat shock response and other inducible systems. Isolation and characterisation of mutants in model fungal and mammalian systems: DNA repair deficiencies associated with heritable diseases (XP, AT etc.): cloning of DNA repair genes: enzymology of eukaryotic DNA repair.
16-18	6	Prof. P. Strike Recombination in prokaryotes and eukaryotes Generalised recombination in E.coli: Holliday model: in vivo evidence for Holliday intermediates. Isolation of rec- mutants: properties of recA, recB recC, recF, recN, sbcA and sbcB: properties of exonuclease V and III: models of recombination pathways. D-loop formation: in vivo properties of RecA protein: strand exchange and transfer: in vivo generation of Holliday structures. The genetics of recombination in yeast: Early stages in meiosis, RecA-like and other recombination proteins from eukaryotes. Transposition in prokaryotes Insertion sequences and compound transposons. The Tn3 family; enzymology of transposition. Bacteriophage
19-21	6	Professor Jon Saunders Invertible control systems Hin flagellar phase variation. Pin/Gin/Cin invertases. Molecular basis of antigenic variability in pathogenic bacteria. Slipped-strand mispairing modulation of bacterial virulence. Genetics and Molecular Biology of Plasmids. Physiology and genetics of bacterial conjugation; trd operon and fertility control. Role of Trd proteins in mating and DNA metabolism. Mobilisation of non-conjugative plasmids. Plasmid structure and function, micro- and macro-evolution. Plasmid replication in relation to cell cycle, amplification, control of copy number and incompatibility. Non-coding RNA and protein controls.