Publications
Selected publications
- Oxygen-regulated post-translation modifications as master signalling pathway in cells. (Journal article - 2023)
- Regulation of chromatin accessibility by hypoxia and HIF (Journal article - 2022)
- Hypoxia Induces Rapid Changes to Histone Methylation and Reprograms Chromatin (Journal article - 2019)
- KDM2 Family Members are Regulated by HIF-1 in Hypoxia (Journal article - 2017)
- Hypoxia and Inflammation in Cancer, Focus on HIF and NF-κB. (Journal article - 2017)
- Potent and selective chemical probe of hypoxic signalling downstream of HIF-α hydroxylation via VHL inhibition. (Journal article - 2016)
- CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells. (Journal article - 2016)
- Hypoxia research, where to now? (Journal article - 2024)
2024
TMT-Based Quantitative Proteomic Profiling of Human Esophageal Cancer Cells Reveals the Potential Mechanism and Potential Therapeutic Targets Associated With Radioresistance.
Gao, A., He, C., Chen, H., Liu, Q., Chen, Y., Sun, J., . . . Zhou, J. (2024). TMT-Based Quantitative Proteomic Profiling of Human Esophageal Cancer Cells Reveals the Potential Mechanism and Potential Therapeutic Targets Associated With Radioresistance.. Proteomics. Clinical applications, e202400010. doi:10.1002/prca.202400010
Autophagy is the main driver of radioresistance of HNSCC cells in mild hypoxia.
Hill, R. M., Li, C., Hughes, J. R., Rocha, S., Grundy, G. J., & Parsons, J. L. (2024). Autophagy is the main driver of radioresistance of HNSCC cells in mild hypoxia.. Journal of cellular and molecular medicine, 28(12), e18482. doi:10.1111/jcmm.18482
Hypoxia research, where to now?
Ortmann, B. M., Taylor, C. T., & Rocha, S. (2024). Hypoxia research, where to now?. Trends in biochemical sciences, S0968-0004(24)00074-4. doi:10.1016/j.tibs.2024.03.008
Global acetylome profiling indicates EPA impedes but OA promotes prostate cancer motility through altered acetylation of PFN1 and FLNA.
He, C., Chen, X., Chen, Y., Sun, J., Qi, M., Rocha, S., & Wang, M. (2024). Global acetylome profiling indicates EPA impedes but OA promotes prostate cancer motility through altered acetylation of PFN1 and FLNA.. Proteomics, e2300393. doi:10.1002/pmic.202300393
2023
The role of autophagy in hypoxia-induced radioresistance.
Hill, R. M., Fok, M., Grundy, G., Parsons, J. L., & Rocha, S. (2023). The role of autophagy in hypoxia-induced radioresistance.. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 189, 109951. doi:10.1016/j.radonc.2023.109951
Comprehensive analysis of the lysine succinylome in fish oil-treated prostate cancer cells.
Jiang, Y., He, C., Ye, H., Xu, Q., Chen, X., Chen, Y., . . . Wang, M. (2023). Comprehensive analysis of the lysine succinylome in fish oil-treated prostate cancer cells.. Life science alliance, 6(11), e202302131. doi:10.26508/lsa.202302131
Enhancing radiotherapy outcomes in rectal cancer: A systematic review of targeting hypoxia-induced radioresistance
Fok, M., Hill, R., Fowler, H., Clifford, R., Kler, A., Uzzi-Daniel, J., . . . Vimalachandran, D. (2023). Enhancing radiotherapy outcomes in rectal cancer: A systematic review of targeting hypoxia-induced radioresistance. Clinical and Translational Radiation Oncology, 44, 100695. doi:10.1016/j.ctro.2023.100695
Oxygen-regulated post-translation modifications as master signalling pathway in cells.
Batie, M., Fasanya, T., Kenneth, N. S., & Rocha, S. (2023). Oxygen-regulated post-translation modifications as master signalling pathway in cells.. EMBO reports, e57849. doi:10.15252/embr.202357849
Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer
Szenasi, A., Sivasudhan, E., Du, H., Zhang, P., Huang, J., Zhang, Z., . . . Wang, M. (2023). Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer. CANCER GENE THERAPY. doi:10.1038/s41417-023-00659-2
Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation.
Shakir, D., Batie, M., & Rocha, S. (2023). Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation.. Methods in molecular biology (Clifton, N.J.), 2675, 261-269. doi:10.1007/978-1-0716-3247-5_19
Functional crosstalk between chromatin and hypoxia signalling
Collier, H., Albanese, A., Kwok, C. -S., Kou, J., & Rocha, S. (2023). Functional crosstalk between chromatin and hypoxia signalling. CELLULAR SIGNALLING, 106. doi:10.1016/j.cellsig.2023.110660
Endotyping COPD: hypoxia-inducible factor-2 as a molecular "switch" between the vascular and airway phenotypes?
Myronenko, O., Foris, V., Crnkovic, S., Olschewski, A., Rocha, S., Nicolls, M. R., & Olschewski, H. (2023). Endotyping COPD: hypoxia-inducible factor-2 as a molecular "switch" between the vascular and airway phenotypes?. European respiratory review : an official journal of the European Respiratory Society, 32(167), 220173. doi:10.1183/16000617.0173-2022
2022
Macrophage Resistance to Ionizing Radiation Exposure Is Accompanied by Decreased Cathepsin D and Increased Transferrin Receptor 1 Expression.
Pinto, A. T., Machado, A. B., Osório, H., Pinto, M. L., Vitorino, R., Justino, G., . . . Oliveira, M. J. (2022). Macrophage Resistance to Ionizing Radiation Exposure Is Accompanied by Decreased Cathepsin D and Increased Transferrin Receptor 1 Expression.. Cancers, 15(1), 270. doi:10.3390/cancers15010270
Overcoming the Impact of Hypoxia in Driving Radiotherapy Resistance in Head and Neck Squamous Cell Carcinoma
Hill, R. M., Rocha, S., & Parsons, J. L. (n.d.). Overcoming the Impact of Hypoxia in Driving Radiotherapy Resistance in Head and Neck Squamous Cell Carcinoma. Cancers, 14(17), 4130. doi:10.3390/cancers14174130
Regulation of chromatin accessibility by hypoxia and HIF
Batie, M., Frost, J., Shakir, D., & Rocha, S. (2022). Regulation of chromatin accessibility by hypoxia and HIF. BIOCHEMICAL JOURNAL, 479(6), 767-786. doi:10.1042/BCJ20220008
Author Correction: Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities.
Teresa Pinto, A., Laranjeiro Pinto, M., Patrícia Cardoso, A., Monteiro, C., Teixeira Pinto, M., Filipe Maia, A., . . . José Oliveira, M. (2022). Author Correction: Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities.. Scientific reports, 12(1), 4774. doi:10.1038/s41598-022-08498-1
Systems approaches to understand oxygen sensing: how multi-omics has driven advances in understanding oxygen-based signalling
Batie, M., Kenneth, N. S., & Rocha, S. (2022). Systems approaches to understand oxygen sensing: how multi-omics has driven advances in understanding oxygen-based signalling. The Biochemical journal, 479(3), 245-257. doi:10.1042/BCJ20210554
2021
Von Hippel-Lindau (VHL) small-molecule inhibitor binding increases stability and intracellular levels of VHL protein
Frost, J., Rocha, S., & Ciulli, A. (2021). Von Hippel-Lindau (VHL) small-molecule inhibitor binding increases stability and intracellular levels of VHL protein. JOURNAL OF BIOLOGICAL CHEMISTRY, 297(2). doi:10.1016/j.jbc.2021.100910
Transcription | Regulation of Gene Transcription by Hypoxia-Inducible Factor 1α
Child, F., Frost, J., Shakir, D., Wilson, J. W., & Rocha, S. (2021). Transcription | Regulation of Gene Transcription by Hypoxia-Inducible Factor 1α. In Encyclopedia of Biological Chemistry III (pp. 480-489). Elsevier. doi:10.1016/b978-0-12-819460-7.00033-5
Oxygen-dependent changes in binding partners and post-translational modifications regulate the abundance and activity of HIF-1 alpha/2 alpha
Daly, L. A., Brownridge, P. J., Batie, M., Rocha, S., See, V., & Eyers, C. E. (2021). Oxygen-dependent changes in binding partners and post-translational modifications regulate the abundance and activity of HIF-1 alpha/2 alpha. SCIENCE SIGNALING, 14(692). doi:10.1126/scisignal.abf6685
Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-kappa B Signaling in Hypoxia and Normoxia
Shakir, D., Batie, M., & Rocha, S. (2021). Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-kappa B Signaling in Hypoxia and Normoxia. NF-(KAPPA)B TRANSCRIPTION FACTORS, 2366, 255-265. doi:10.1007/978-1-0716-1669-7_15
PBRM1 Cooperates with YTHDF2 to Control HIF-1 alpha Protein Translation
Shmakova, A., Frost, M., Batie, M., Kenneth, N. S., & Rocha, S. (2021). PBRM1 Cooperates with YTHDF2 to Control HIF-1 alpha Protein Translation. CELLS, 10(6). doi:10.3390/cells10061425
Role of Hypoxia in the Control of the Cell Cycle
Druker, J., Wilson, J. W., Child, F., Shakir, D., Fasanya, T., & Rocha, S. (2021). Role of Hypoxia in the Control of the Cell Cycle. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(9). doi:10.3390/ijms22094874
PBRM1 Cooperates with YTHDF2 to Control HIF-1Alpha Protein Translation
Shmakova, A., Frost, M., Kenneth, N., & Rocha, S. (2021). PBRM1 Cooperates with YTHDF2 to Control HIF-1Alpha Protein Translation. doi:10.20944/preprints202104.0359.v1
VHL ligand binding increases intracellular level of VHL
Frost, J., Rocha, S., & Ciulli, A. (2021). VHL ligand binding increases intracellular level of VHL. doi:10.1101/2021.04.12.439487
Roles of HIF and 2-Oxoglutarate-Dependent Dioxygenases in Controlling Gene Expression in Hypoxia
Frost, J., Frost, M., Batie, M., Jiang, H., & Rocha, S. (2021). Roles of HIF and 2-Oxoglutarate-Dependent Dioxygenases in Controlling Gene Expression in Hypoxia. CANCERS, 13(2). doi:10.3390/cancers13020350
2020
Roles Of HIF and 2-Oxoglutarate Dependent Enzymes in Controlling Gene Expression In Hypoxia
Frost, J., Frost, M., Batie, M., Jiang, H., & Rocha, S. (2020). Roles Of HIF and 2-Oxoglutarate Dependent Enzymes in Controlling Gene Expression In Hypoxia. doi:10.20944/preprints202011.0651.v1
Oxygen-sensing mechanisms in cells
Wilson, J. W., Shakir, D., Batie, M., Frost, M., & Rocha, S. (2020). Oxygen-sensing mechanisms in cells. FEBS JOURNAL, 287(18), 3888-3906. doi:10.1111/febs.15374
Gene transcription and chromatin regulation in hypoxia
Batie, M., & Rocha, S. (2020). Gene transcription and chromatin regulation in hypoxia. Biochemical Society transactions, 48(3), 1121-1128. doi:10.1042/bst20191106
HIF-1β Positively Regulates NF-κB Activity via Direct Control of TRAF6
D'Ignazio, L., Shakir, D., Batie, M., Muller, H. A., & Rocha, S. (2020). HIF-1β Positively Regulates NF-κB Activity via Direct Control of TRAF6. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(8). doi:10.3390/ijms21083000
2019
JmjC histone demethylases act as chromatin oxygen sensors
Batie, M., & Rocha, S. (2019). JmjC histone demethylases act as chromatin oxygen sensors. Molecular and Cellular Oncology. doi:10.1080/23723556.2019.1608501
Hypoxia Induces Rapid Changes to Histone Methylation and Reprograms Chromatin
Batie, M., Frost, J., Frost, M., Wilson, J. W., Schofield, P., & Rocha, S. (2019). Hypoxia Induces Rapid Changes to Histone Methylation and Reprograms Chromatin. Science, 363(6432), 1222-1226. doi:10.1126/science.aau5870
Hypoxia induces rapid changes to histone methylation reprogramming chromatin for the cellular response: Supplementary material
Batie, M., Frost, J., Frost, M., Wilson, J., Schofield, P., & Rocha, S. (2019). Hypoxia induces rapid changes to histone methylation reprogramming chromatin for the cellular response: Supplementary material. doi:10.1101/513069
RNA-seq analysis of PHD and VHL inhibitors reveals differences and similarities to the hypoxia response.
Frost, J., Ciulli, A., & Rocha, S. (2019). RNA-seq analysis of PHD and VHL inhibitors reveals differences and similarities to the hypoxia response.. Wellcome open research, 4, 17. doi:10.12688/wellcomeopenres.15044.1
2018
TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway
D'Ignazio, L., Batie, M., & Rocha, S. (2018). TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway. CELLS, 7(8). doi:10.3390/cells7080102
TNFSF14/LIGHT, a Non-Canonical NF-kB Stimulus, Induces the HIF Pathway
D'Ignazio, L., Batie, M., & Rocha, S. (2018). TNFSF14/LIGHT, a Non-Canonical NF-kB Stimulus, Induces the HIF Pathway. doi:10.20944/preprints201807.0007.v1
SINHCAF/FAM60A and SIN3A specifically repress HIF-2α expression
Biddlestone, J., Batie, M., Bandarra, D., Munoz, I., & Rocha, S. (2018). SINHCAF/FAM60A and SIN3A specifically repress HIF-2α expression. BIOCHEMICAL JOURNAL, 475, 2073-2090. doi:10.1042/BCJ20170945
Hypoxia and Chromatin: A Focus on Transcriptional Repression Mechanisms
Batie, M., del Peso, L., & Rocha, S. (2018). Hypoxia and Chromatin: A Focus on Transcriptional Repression Mechanisms. BIOMEDICINES, 6(2). doi:10.3390/biomedicines6020047
Hypoxia and Chromatin, a Focus on Transcriptional Repression Mechanisms
Batie, M., del Peso, L., & Rocha, S. (2018). Hypoxia and Chromatin, a Focus on Transcriptional Repression Mechanisms. doi:10.20944/preprints201803.0004.v1
Group-Based Optimization of Potent and Cell-Active Inhibitors of the von Hippel–Lindau (VHL) E3 Ubiquitin Ligase: Structure–Activity Relationships Leading to the Chemical Probe (2 S ,4 R )-1-(( S )-2-(1-Cyanocyclopropanecarboxamido)-3,3-dimethylbutanoyl)-4-hydroxy- N -(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide (VH298)
Soares, P., Gadd, M. S., Frost, J., Galdeano, C., Ellis, L., Epemolu, O., . . . Ciulli, A. (2018). Group-Based Optimization of Potent and Cell-Active Inhibitors of the von Hippel-Lindau (VHL) E3 Ubiquitin Ligase: Structure-Activity Relationships Leading to the Chemical Probe (2<i>S</i>,4<i>R</i>)-1-((<i>S</i>)-2-(1-Cyanocyclopropanecarboxamido)-3,3-dimethylbutanoyI)-4-hydroxy-<i>N</i>-(4-(4-methylthiazol-5-yl)benzyppyrrolidine-2-carboxamide (VH298). JOURNAL OF MEDICINAL CHEMISTRY, 61(2), 599-618. doi:10.1021/acs.jmedchem.7b00675
2017
Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation.
Maniaci, C., Hughes, S. J., Testa, A., Chen, W., Lamont, D. J., Rocha, S., . . . Ciulli, A. (2017). Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation.. Nature communications, 8. doi:10.1038/s41467-017-00954-1
Hypoxia and Inflammation in Cancer, Focus on HIF and NF-κB.
D'Ignazio, L., Batie, M., & Rocha, S. (2017). Hypoxia and Inflammation in Cancer, Focus on HIF and NF-κB.. Biomedicines, 5(2), E21. doi:10.3390/biomedicines5020021
Hypoxia and Inflammation in Cancer, Focus on HIF and NF-кB
D'Ignazio, L., Batie, M., & Rocha, S. (2017). Hypoxia and Inflammation in Cancer, Focus on HIF and NF-кB. doi:10.20944/preprints201703.0240.v1
KDM2 Family Members are Regulated by HIF-1 in Hypoxia
Batie, M., Druker, J., D'Ignazio, L., & Rocha, S. (2017). KDM2 Family Members are Regulated by HIF-1 in Hypoxia. CELLS, 6(1). doi:10.3390/cells6010008
2016
Potent and selective chemical probe of hypoxic signalling downstream of HIF-α hydroxylation via VHL inhibition.
Frost, J., Galdeano, C., Soares, P., Gadd, M. S., Grzes, K. M., Ellis, L., . . . Ciulli, A. (2016). Potent and selective chemical probe of hypoxic signalling downstream of HIF-α hydroxylation via VHL inhibition.. Nature communications, 7, 12 pages. doi:10.1038/ncomms13312
Intricate Macrophage-Colorectal Cancer Cell Communication in Response to Radiation.
Pinto, A. T., Pinto, M. L., Velho, S., Pinto, M. T., Cardoso, A. P., Figueira, R., . . . Rocha, S. (2016). Intricate Macrophage-Colorectal Cancer Cell Communication in Response to Radiation.. PloS one, 11(8), e0160891. doi:10.1371/journal.pone.0160891
Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell Lines.
Ono, M., Yamada, K., Bensaddek, D., Afzal, V., Biddlestone, J., Ortmann, B., . . . Lamond, A. I. (2016). Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell Lines.. PloS one, 11(4), e0154759. doi:10.1371/journal.pone.0154759
Hypoxia Induced NF-κB.
D'Ignazio, L., & Rocha, S. (2016). Hypoxia Induced NF-κB.. Cells, 5(1), E10. doi:10.3390/cells5010010
The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors
Wiechens, N., Singh, V., Gkikopoulos, T., Schofield, P., Rocha, S., & Owen-Hughes, T. (2016). The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors. PLOS GENETICS, 12(3). doi:10.1371/journal.pgen.1005940
Erratum.
Erratum. (2016). Autophagy, 12(2), 443. doi:10.1080/15548627.2016.1147886
NF-κB and HIF crosstalk in immune responses.
D'Ignazio, L., Bandarra, D., & Rocha, S. (2016). NF-κB and HIF crosstalk in immune responses.. The FEBS journal, 283(3), 413-424. doi:10.1111/febs.13578
Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities.
Teresa Pinto, A., Laranjeiro Pinto, M., Patrícia Cardoso, A., Monteiro, C., Teixeira Pinto, M., Filipe Maia, A., . . . José Oliveira, M. (2016). Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities.. Scientific reports, 6, 18765. doi:10.1038/srep18765
CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells.
Ortmann, B., Bensaddek, D., Carvalhal, S., Moser, S. C., Mudie, S., Griffis, E. R., . . . Rocha, S. (2016). CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells.. Journal of cell science, 129(1), 191-205. doi:10.1242/jcs.179911
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., . . . Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). AUTOPHAGY, 12(1), 1-222. doi:10.1080/15548627.2015.1100356
2015
The Prolylhydroxylase PHD2 Modulates Centromere Function through the Hydroxylation of CENP-N
Moser, S., Bensaddek, D., Ortmann, B., Rocha, S., Lamond, A., & Swedlow, J. (2015). The Prolylhydroxylase PHD2 Modulates Centromere Function through the Hydroxylation of CENP-N. doi:10.1101/030452
Cezanne regulates E2F1-dependent HIF2α expression.
Moniz, S., Bandarra, D., Biddlestone, J., Campbell, K. J., Komander, D., Bremm, A., & Rocha, S. (2015). Cezanne regulates E2F1-dependent HIF2α expression.. Journal of Cell Science, 128(16), 3082-3093. doi:10.1242/jcs.168864
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
Sun, Y., George, J., George, J., & Rocha, S. (2015). Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.. PloS one, 10(4), e0123649. doi:10.1371/journal.pone.0123649
The role of hypoxia in inflammatory disease (review).
Biddlestone, J., Bandarra, D., & Rocha, S. (2015). The role of hypoxia in inflammatory disease (review).. International journal of molecular medicine, 35(4), 859-869. doi:10.3892/ijmm.2015.2079
HIF-1α restricts NF-κB-dependent gene expression to control innate immunity signals
Bandarra, D., Biddlestone, J., Mudie, S., Müller, H. -A. J., & Rocha, S. (2015). HIF-1α restricts NF-κB-dependent gene expression to control innate immunity signals. Disease Models and Mechanisms, 8(2), 169-181. doi:10.1242/dmm.017285
2014
PITX1, a specificity determinant in the HIF-1α-mediated transcriptional response to hypoxia
Mudie, S., Bandarra, D., Batie, M., Biddlestone, J., Moniz, S., Ortmann, B., . . . Rocha, S. (2014). PITX1, a specificity determinant in the HIF-1α-mediated transcriptional response to hypoxia. CELL CYCLE, 13(24), 3878-3891. doi:10.4161/15384101.2014.972889
Cezanne (OTUD7B) regulates HIF-1α homeostasis in a proteasome-independent manner
Bremm, A., Moniz, S., Mader, J., Rocha, S., & Komander, D. (2014). Cezanne (OTUD7B) regulates HIF-1α homeostasis in a proteasome-independent manner. EMBO Reports, 15(12), 1268-1277. doi:10.15252/embr.201438850
Chromatin and oxygen sensing in the context of JmjC histone demethylases
Shmakova, A., Batie, M., Druker, J., & Rocha, S. (2014). Chromatin and oxygen sensing in the context of JmjC histone demethylases. BIOCHEMICAL JOURNAL, 462, 385-395. doi:10.1042/BJ20140754
Hypoxia activates IKK-NF-κB and the immune response in Drosophila melanogaster.
Bandarra, D., Biddlestone, J., Mudie, S., Muller, H. A., & Rocha, S. (2014). Hypoxia activates IKK-NF-κB and the immune response in Drosophila melanogaster.. Bioscience reports, 34(4), e00127. doi:10.1042/bsr20140095
SiRNA screening to identify ubiquitin and ubiquitin-like system regulators of biological pathways in cultured mammalian cells.
Bett, J. S., Ibrahim, A. F. M., Garg, A. K., Rocha, S., & Hay, R. T. (2014). SiRNA screening to identify ubiquitin and ubiquitin-like system regulators of biological pathways in cultured mammalian cells.. Journal of visualized experiments : JoVE, (87). doi:10.3791/51572
Analysis of global RNA synthesis at the single cell level following hypoxia.
Biddlestone, J., Druker, J., Shmakova, A., Ferguson, G., Swedlow, J. R., & Rocha, S. (2014). Analysis of global RNA synthesis at the single cell level following hypoxia.. Journal of visualized experiments : JoVE, (87). doi:10.3791/51420
Cell cycle progression in response to oxygen levels.
Ortmann, B., Druker, J., & Rocha, S. (2014). Cell cycle progression in response to oxygen levels.. Cellular and molecular life sciences : CMLS, 71(18), 3569-3582. doi:10.1007/s00018-014-1645-9
Grow₂: the HIF system, energy homeostasis and the cell cycle.
Moniz, S., Biddlestone, J., & Rocha, S. (2014). Grow₂: the HIF system, energy homeostasis and the cell cycle.. Histology and histopathology, 29(5), 589-600. doi:10.14670/hh-29.10.589
2013
HIF-independent role of prolyl hydroxylases in the cellular response to amino acids.
Durán, R. V., MacKenzie, E. D., Boulahbel, H., Frezza, C., Heiserich, L., Tardito, S., . . . Gottlieb, E. (2013). HIF-independent role of prolyl hydroxylases in the cellular response to amino acids.. Oncogene, 32(38), 4549-4556. doi:10.1038/onc.2012.465
PHD1 Links Cell-Cycle Progression to Oxygen Sensing through Hydroxylation of the Centrosomal Protein Cep192
Moser, S. C., Bensaddek, D., Ortmann, B., Maure, J. -F., Mudie, S., Blow, J. J., . . . Rocha, S. (2013). PHD1 Links Cell-Cycle Progression to Oxygen Sensing through Hydroxylation of the Centrosomal Protein Cep192. DEVELOPMENTAL CELL, 26(4), 381-392. doi:10.1016/j.devcel.2013.06.014
FMN2 is a novel regulator of the cyclin-dependent kinase inhibitor p21
Yamada, K., Ono, M., Bensaddek, D., Lamond, A. I., & Rocha, S. (2013). FMN2 is a novel regulator of the cyclin-dependent kinase inhibitor p21. CELL CYCLE, 12(15), 2348-2354. doi:10.4161/cc.25511
The P-body component USP52/PAN2 is a novel regulator of <i>HIF1A</i> mRNA stability
Bett, J. S., Ibrahim, A. F. M., Garg, A. K., Kelly, V., Pedrioli, P., Rocha, S., & Hay, R. T. (2013). The P-body component USP52/PAN2 is a novel regulator of <i>HIF1A</i> mRNA stability. BIOCHEMICAL JOURNAL, 451, 185-194. doi:10.1042/BJ20130026
Identification and Functional Characterization of FMN2, a Regulator of the Cyclin-Dependent Kinase Inhibitor p21
Yamada, K., Ono, M., Perkins, N. D., Rocha, S., & Lamond, A. I. (2013). Identification and Functional Characterization of FMN2, a Regulator of the Cyclin-Dependent Kinase Inhibitor p21. MOLECULAR CELL, 49(5), 922-933. doi:10.1016/j.molcel.2012.12.023
TfR1 interacts with the IKK complex and is involved in IKK-NF-κB signalling
Kenneth, N. S., Mudie, S., Naron, S., & Rocha, S. (2013). TfR1 interacts with the IKK complex and is involved in IKK-NF-κB signalling. BIOCHEMICAL JOURNAL, 449, 275-284. doi:10.1042/BJ20120625
2012
Family with Sequence Similarity 60A (FAM60A) Protein Is a Cell Cycle-fluctuating Regulator of the SIN3-HDAC1 Histone Deacetylase Complex
Munoz, I. M., MacArtney, T., Sanchez-Pulido, L., Ponting, C. P., Rocha, S., & Rouse, J. (2012). Family with Sequence Similarity 60A (FAM60A) Protein Is a Cell Cycle-fluctuating Regulator of the SIN3-HDAC1 Histone Deacetylase Complex. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(39), 32346-32353. doi:10.1074/jbc.M112.382499
Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway
Arabi, A., Ullah, K., Branca, R. M. M., Johansson, J., Bandarra, D., Haneklaus, M., . . . Sangfelt, O. (2012). Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway. NATURE COMMUNICATIONS, 3. doi:10.1038/ncomms1975
IKK and NF-κB-mediated regulation of Claspin impacts on ATR checkpoint function
IKK and NF-κB-mediated regulation of Claspin impacts on ATR checkpoint function (2012). The EMBO journal, 31(11), 2660-2661. doi:10.1038/emboj.2012.135
Chromatin as an oxygen sensor and active player in the hypoxia response
Melvin, A., & Rocha, S. (2012). Chromatin as an oxygen sensor and active player in the hypoxia response. CELLULAR SIGNALLING, 24(1), 35-43. doi:10.1016/j.cellsig.2011.08.019
2011
The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH
Melvin, A., Mudie, S., & Rocha, S. (2011). The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH. MOLECULAR BIOLOGY OF THE CELL, 22(21), 4171-4181. doi:10.1091/mbc.E11-02-0163
The role of RelA (p65) threonine 505 phosphorylation in the regulation of cell growth, survival, and migration
Msaki, A., Sanchez, A. M., Koh, L. F., Barre, B., Rocha, S., Perkins, N. D., & Johnson, R. F. (2011). The role of RelA (p65) threonine 505 phosphorylation in the regulation of cell growth, survival, and migration. MOLECULAR BIOLOGY OF THE CELL, 22(17), 3032-3040. doi:10.1091/mbc.E11-04-0280
NF-κB controls energy homeostasis and metabolic adaptation by upregulating mitochondrial respiration
Mauro, C., Leow, S. C., Anso, E., Rocha, S., Thotakura, A. K., Tornatore, L., . . . Franzoso, G. (2011). NF-κB controls energy homeostasis and metabolic adaptation by upregulating mitochondrial respiration. NATURE CELL BIOLOGY, 13(10), 1272-U234. doi:10.1038/ncb2324
[Erratum: Volume 10, Issue 6, pp. 879–82] Mechanism of hypoxia-induced NFkB
[Erratum: Volume 10, Issue 6, pp. 879–82] Mechanism of hypoxia-induced NFkB (2011). Cell cycle (Georgetown, Tex.), 10(12), 2041. doi:10.4161/cc.10.12.16720
Antagonistic crosstalk between APC and HIF-1α
Naethke, I., & Rocha, S. (2011). Antagonistic crosstalk between APC and HIF-1α. CELL CYCLE, 10(10), 1545-1547. doi:10.4161/cc.10.10.15638
HIF-1α depletion results in SP1-mediated cell cycle disruption and alters the cellular response to chemotherapeutic drugs
Culver, C., Melvin, A., Mudie, S., & Rocha, S. (2011). HIF-1α depletion results in SP1-mediated cell cycle disruption and alters the cellular response to chemotherapeutic drugs. CELL CYCLE, 10(8), 1249-1260. doi:10.4161/cc.10.8.15326
Mechanism of hypoxia-induced NFκB
Melvin, A., Mudie, S., & Rocha, S. (2011). Mechanism of hypoxia-induced NFκB. CELL CYCLE, 10(6), 879-882. doi:10.4161/cc.10.6.14910
Evolutionary Conserved Regulation of HIF-1β by NF-κB
van Uden, P., Kenneth, N. S., Webster, R., Mueller, H. A., Mudie, S., & Rocha, S. (2011). Evolutionary Conserved Regulation of HIF-1β by NF-κB. PLOS GENETICS, 7(1). doi:10.1371/journal.pgen.1001285
2010
Adenomatous Polyposis Coli and Hypoxia-inducible Factor-1α Have an Antagonistic Connection
Newton, I. P., Kenneth, N. S., Appleton, P. L., Naethke, I., & Rocha, S. (2010). Adenomatous Polyposis Coli and Hypoxia-inducible Factor-1α Have an Antagonistic Connection. MOLECULAR BIOLOGY OF THE CELL, 21(21), 3630-3638. doi:10.1091/mbc.E10-04-0312
Mechanism of Hypoxia-Induced NF-κB
Culver, C., Sundqvist, A., Mudie, S., Melvin, A., Xirodimas, D., & Rocha, S. (2010). Mechanism of Hypoxia-Induced NF-κB. MOLECULAR AND CELLULAR BIOLOGY, 30(20), 4901-4921. doi:10.1128/MCB.00409-10
IKK and NF-κB-mediated regulation of Claspin impacts on ATR checkpoint function
Kenneth, N. S., Mudie, S., & Rocha, S. (2010). IKK and NF-κB-mediated regulation of Claspin impacts on ATR checkpoint function. EMBO JOURNAL, 29(17), 2966-2978. doi:10.1038/emboj.2010.171
2009
SWI/SNF Regulates the Cellular Response to Hypoxia
Kenneth, N. S., Mudie, S., van Uden, P., & Rocha, S. (2009). SWI/SNF Regulates the Cellular Response to Hypoxia. JOURNAL OF BIOLOGICAL CHEMISTRY, 284(7), 4123-4131. doi:10.1074/jbc.M808491200
2008
Regulation of gene expression by hypoxia
Kenneth, N. S., & Rocha, S. (2008). Regulation of gene expression by hypoxia. BIOCHEMICAL JOURNAL, 414, 19-29. doi:10.1042/BJ20081055
Regulation of hypoxia-inducible factor-1α by NF-κB
van Uden, P., Kenneth, N. S., & Rocha, S. (2008). Regulation of hypoxia-inducible factor-1α by NF-κB. BIOCHEMICAL JOURNAL, 412, 477-484. doi:10.1042/BJ20080476
2007
Gene regulation under low oxygen: holding your breath for transcription
Rocha, S. (2007). Gene regulation under low oxygen: holding your breath for transcription. TRENDS IN BIOCHEMICAL SCIENCES, 32(8), 389-397. doi:10.1016/j.tibs.2007.06.005
Regulation of ATR-dependent pathways by the FHA domain containing protein SNIP1
Roche, K. C., Rocha, S., Bracken, C. P., & Perkins, N. D. (2007). Regulation of ATR-dependent pathways by the FHA domain containing protein SNIP1. ONCOGENE, 26(31), 4523-4530. doi:10.1038/sj.onc.1210233
2006
SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes
Fujii, M., Lyakh, L. A., Bracken, C. P., Fukuoka, J., Hayakawa, M., Tsukiyama, T., . . . Roberts, A. B. (2006). SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes. MOLECULAR CELL, 24(5), 771-783. doi:10.1016/j.molcel.2006.11.006
Regulation of p53 tumour suppressor target gene expression by the p52 NF-κB subunit
Schumm, K., Rocha, S., Caamano, J., & Perkins, N. D. (2006). Regulation of p53 tumour suppressor target gene expression by the p52 NF-κB subunit. EMBO JOURNAL, 25(20), 4820-4832. doi:10.1038/sj.emboj.7601343
Cisplatin mimics ARF tumor suppressor regulation of RelA (p65) nuclear factor-κB transactivation
Campbell, K. J., Witty, J. M., Rocha, S., & Perkins, N. D. (2006). Cisplatin mimics ARF tumor suppressor regulation of RelA (p65) nuclear factor-κB transactivation. CANCER RESEARCH, 66(2), 929-935. doi:10.1158/0008-5472.CAN-05-2234
2005
ARF the integrator -: Linking NF-κB, p53 and checkpoint kinases
Rocha, S., & Perkins, N. D. (2005). ARF the integrator -: Linking NF-κB, p53 and checkpoint kinases. CELL CYCLE, 4(6), 756-759. doi:10.4161/cc.4.6.1739
Regulation of NF-κB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor
Rocha, S., Garrett, M. D., Campbell, K. J., Schumm, K., & Perkins, N. D. (2005). Regulation of NF-κB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor. EMBO JOURNAL, 24(6), 1157-1169. doi:10.1038/sj.emboj.7600608
2004
Active repression of antiapoptotic gene expression by ReIA(p65) NF-κB
Campbell, K. J., Rocha, S., & Perkins, N. D. (2004). Active repression of antiapoptotic gene expression by ReIA(p65) NF-κB. MOLECULAR CELL, 13(6), 853-865. doi:10.1016/S1097-2765(04)00131-5
2003
The p53-inhibitor Pifithrin-α inhibits Firefly Luciferase activity <i>in vivo</i> and <i>in vitro</i> -: art. no. 9
Rocha, S., Campbell, K. J., Roche, K. C., & Perkins, N. D. (2003). The p53-inhibitor Pifithrin-α inhibits Firefly Luciferase activity <i>in vivo</i> and <i>in vitro</i> -: art. no. 9. BMC MOLECULAR BIOLOGY, 4. doi:10.1186/1471-2199-4-9
p53 Represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-κB subunit with histone deacetylase 1
Rocha, S., Martin, A. M., Meek, D. W., & Perkins, N. D. (2003). p53 Represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-κB subunit with histone deacetylase 1. MOLECULAR AND CELLULAR BIOLOGY, 23(13), 4713-4727. doi:10.1128/MCB.23.13.4713-4727.2003
p53- and Mdm2-independent repression of NF-κB transactivation by the ARF tumor suppressor
Rocha, S., Campbell, K. J., & Perkins, N. D. (2003). p53- and Mdm2-independent repression of NF-κB transactivation by the ARF tumor suppressor. MOLECULAR CELL, 12(1), 15-25. doi:10.1016/S1097-2765(03)00223-5
Recombinant mistletoe lectin induces p53-independent apoptosis in tumour cells and cooperates with ionising radiation
Hostanska, K., Vuong, V., Rocha, S., Soengas, M. S., Glanzmann, C., Saller, R., . . . Pruschy, M. (2003). Recombinant mistletoe lectin induces p53-independent apoptosis in tumour cells and cooperates with ionising radiation. BRITISH JOURNAL OF CANCER, 88(11), 1785-1792. doi:10.1038/sj.bjc.6600982
2002
NF-κB Function in Inflammation, Cellular Stress and Disease
Chapman, N. R., Rocha, S., Adcock, I. M., & Perkins, N. D. (2002). NF-κB Function in Inflammation, Cellular Stress and Disease. Unknown Journal, 61-73. doi:10.1016/s1568-1254(02)80007-9
2001
The phosphatidylinositide 3′-kinase/Akt survival pathway is a target for the anticancer and radiosensitizing agent PKC412, an inhibitor of protein kinase C
Tenzer, A., Zingg, D., Rocha, S., Hemmings, B., Fabbro, D., Glanzmann, C., . . . Prusch, M. (2001). The phosphatidylinositide 3′-kinase/Akt survival pathway is a target for the anticancer and radiosensitizing agent PKC412, an inhibitor of protein kinase C. CANCER RESEARCH, 61(22), 8203-8210. Retrieved from https://www.webofscience.com/
Overexpression of Bcl-2 enhances sensitivity of L929 cells to a lipophilic cationic photosensitiser.
Klein, S. D., Walt, H., Rocha, S., Ghafourifar, P., Pruschy, M., Winterhalter, K. H., & Richter, C. (2001). Overexpression of Bcl-2 enhances sensitivity of L929 cells to a lipophilic cationic photosensitiser.. Cell death and differentiation, 8(2), 204-206. doi:10.1038/sj.cdd.4400814
Key targets for the execution of radiation-induced tumor cell apoptosis: The role of p53 and caspases
Pruschy, M., Rocha, S., Zaugg, K., Tenzer, A., Hess, C., Fisher, D. E., . . . Bodis, S. (2001). Key targets for the execution of radiation-induced tumor cell apoptosis: The role of p53 and caspases. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 49(2), 561-567. doi:10.1016/S0360-3016(00)01480-2
Differential p53-dependent mechanism of radiosensitization <i>in vitro</i> and <i>in vivo</i> by the protein kinase C-specific inhibitor PKC412
Zaugg, K., Rocha, S., Resch, H., Hegyi, I., Oehler, C., Glanzmann, C., . . . Pruschy, M. (2001). Differential p53-dependent mechanism of radiosensitization <i>in vitro</i> and <i>in vivo</i> by the protein kinase C-specific inhibitor PKC412. CANCER RESEARCH, 61(2), 732-738. Retrieved from https://www.webofscience.com/
2000
Protein kinase C inhibitor and irradiation-induced apoptosis:: Relevance of the cytochrome <i>c</i>-mediated caspase-9 death pathway
Rocha, S., Soengas, M. S., Lowe, S. W., Glanzmann, C., Fabbro, D., Winterhalter, K., . . . Pruschy, M. (2000). Protein kinase C inhibitor and irradiation-induced apoptosis:: Relevance of the cytochrome <i>c</i>-mediated caspase-9 death pathway. CELL GROWTH & DIFFERENTIATION, 11(9), 491-499. Retrieved from https://www.webofscience.com/
1999
Ceramide induces cytochrome <i>c</i> release from isolated mitochondria -: Importance of mitochondrial redox state
Ghafourifar, P., Klein, S. D., Schucht, O., Schenk, U., Pruschy, M., Rocha, S., & Richter, C. (1999). Ceramide induces cytochrome <i>c</i> release from isolated mitochondria -: Importance of mitochondrial redox state. JOURNAL OF BIOLOGICAL CHEMISTRY, 274(10), 6080-6084. doi:10.1074/jbc.274.10.6080