Research
Research Interests
His main areas of research are in the areas of protein chemistry, proteomics, proteolysis, and proteolytic. In particular, his research programmes are dedicated to the application of modern methods of protein analysis to problems in biology. He has pioneered the areas of understanding protein dynamics on a global, proteome wide scale and of developing relevant methods in intact animals and complex systems. He has pioneered the areas of understanding protein dynamics on a global, proteome wide scale and of developing relevant methods in intact animals and complex systems. With colleagues, he developed a wholly new strategy for multiplexed absolute quantification in proteomics (QconCAT) that has been applied to diverse areas such as muscle development and expression of polymorphic proteins in chemical communication. He has described a new strategy for rational proteome simplification that has created new opportunities for rapid proteome profiling. His website for the calculation of thermodynamically correct buffers (www.liv.ac.uk/buffers) has helped the scientific community to prepare over three quarters of a million litres of buffers!
Research grants
ALACATS: bespoke strategy for absolute protein quantification
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL, ROSALIND FRANKLIN INSTITUTE (UK)
July 2019 - September 2022
Unlocking the molecular stoichiometry of functional CO2-fixing organelles for synthetic engineering
ROYAL SOCIETY
October 2018 - March 2022
The effect of Gastro-oesophageal reflux on CF lung disease
VERTEX PHARMACEUTICALS INC (USA)
June 2017 - August 2021
KTP with Badrilla Ltd
BADRILLA LTD (UK), INNOVATE UK (UK)
February 2018 - January 2020
Use of REIMS in ecological analysis
MICROMASS UK LIMITED (UK)
October 2017 - March 2022
Double standards in quantitative proteomics: Development of calibrators for multiplexed quantitative western blotting or mass spectrometry
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
May 2015 - April 2016
Role of serine protease activity in the pathogenesis of muscular dystrophy
MUSCULAR DYSTROPHY ASSOCIATION (USA)
September 2015 - January 2019
Understanding and exploiting the urinary proteins of New Zealand's leading mammal pest
ROYAL SOCIETY OF NEW ZEALAND (NEW ZEALAND)
February 2017 - October 2019
analysis of MUPs in Mus species and hybrids”
FRENCH NATIONAL CENTRE FOR SCIENTIFIC RESEARCH (CNRS) (FR)
September 2015 - July 2020
Bayesian Quantitative Proteomics
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
September 2015 - March 2019
Multiplexed Efficient Expression of Recombinant Qconcats
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
June 2017 - July 2017
Unilever Framework Grant
UNILEVER UK CENTRAL RESOURCES LTD (UK)
July 2015 - July 2019
Development of a quantitative assay for pepsin in bronchoalveolar lavage (BAL) as a tool for clinical diagnoses
BIOCHEMICAL SOCIETY (UK)
July 2016 - August 2016
Sparking Impact
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
June 2013 - July 2014
YEAR 1 Wellcome Trust ISSF Non-Clinical Fellowships
WELLCOME TRUST (UK)
October 2011 - May 2018
A new form of scent communication in house mice: navigation cues.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
October 2001 - April 2005
Identification of vaccine candidates in larval excretory/secretory products of Teladorsagia circumcincta.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
January 2007 - December 2009
BIOMAH - Novel strategies for biomarker discovery and quantification
EUROPEAN COMMISSION
April 2009 - May 2011
SILAC analysis of the HGF signalling.
CANCER RESEARCH UK (UK)
October 2005 - September 2008
FPGA supercomputing technology for high-throughput identification and quantitation in proteomics
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
March 2008 - March 2011
Rapid proteome profiling using positional signature peptides
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
June 2008 - May 2010
Northwest consortium for third generation proteomics.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
November 2001 - November 2004
The interplay of rodent behaviour, semiochemistry and systems: from scientific principles to control strategy
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
July 2012 - July 2018
Proposal to Establish a Bioinformatics Laboratory.
WOLFSON FOUNDATION (UK)
January 2001 - July 2001
Applying innovative technologies to improve the benefit-risk ratio of drugs: developing a national resource underpinned by the infrastructure of the MRC Centre for Drug Safety Science
MEDICAL RESEARCH COUNCIL
April 2015 - March 2016
MUP knockout mice: implications for chemical communication and a generic research tool.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
May 2006 - July 2010
Double standards in proteomics
BADRILLA LTD (UK), BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
March 2012 - July 2012
The Liverpool Bespoke Oligoarray Facility (L-BOF).
NATURAL ENVIRONMENT RESEARCH COUNCIL
January 2005 - January 2008
Proteomics analysis of keratinocyte. A Preliminary Investigation
UNILEVER PLC (UK)
October 2013 - November 2013
Partnership in applied proteomic technologies: understanding the academic/industrial perspectives.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
June 2010 - November 2012
Linking the proteome and transcriptome - assessment of the relationship between proteome ubiquitination and turnover.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
October 2004 - September 2007
MUPs, MHC and mate choice in house mice.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
May 2004 - April 2008
Myofibroblast control of oesophageal squamous cancer invasion: role of chemerin
NORTH WEST CANCER RESEARCH FUND, NORTH WEST CANCER RESEARCH INCORPORATING CLATTERBRIDGE CANCER RESEARCH (UK)
February 2015 - December 2018
Characterisation of two antigen complexes for use in a diagnostic assay for larval stage cyathostomins.
THE HORSE TRUST (UK)
March 2003 - February 2004
Application of advanced MS instrumentation to protein ligand binding and PTM characterisation
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
January 2015 - January 2016
Dynamics and function of the NF-kappaB signalling system
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
May 2008 - July 2014
Ecoyeast - Mastering the economics of adaptation through constraint-based modeling in yeast
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
March 2015 - June 2018
Double Standards in Proteomics
BADRILLA LTD (UK)
October 2012 - September 2016
The decision to cooperate: kin recognition mechanisms in female house mice
NATURAL ENVIRONMENT RESEARCH COUNCIL
March 2010 - March 2014
Exploiting Genomic Information: Strategies for Cross-Species Identification in Proteomics.
NATURAL ENVIRONMENT RESEARCH COUNCIL
January 2002 - September 2005
Bio-Chemical and Behavioural Mechanisms of Olefactory Communication in Wild House Mice.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
February 2000 - March 2001
Investigating cartilage matrix turnover in health and disease using a quantitative proteomics approach
WELLCOME TRUST (UK)
September 2009 - September 2015
Identification of candidate protective antigens for vaccination against the bovine lungworm, Dictyocaulus viviparus.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
March 2003 - March 2006
A proteomics approach to quantify adaptive variation in mammalian ejaculates
NATURAL ENVIRONMENT RESEARCH COUNCIL
January 2012 - December 2014
Molecular changes that characterise the unique response of North Ronaldsay sheep to copper challenge compared with domesticated (Cambridge) sheep.
WELLCOME TRUST (UK)
July 2001 - January 2004
Automatic monitoring and control of small mammals in large and complex spaces.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
April 2003 - April 2006
The PPP-labels software for quantitative proteomics
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
September 2014 - September 2015
Mass spectrometry imaging for biology and biotechnology
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
January 2014 - December 2014
Keeping up with the bases: 'nextgen' approaches to proteomics
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
July 2013 - July 2014
Turnover profiling: commitment and degradation of the yeast proteome.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
January 2001 - December 2004
A systems biology study of E2F and NF-kappaB cross-talk
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
February 2010 - April 2014
Hair proteomics and metabolomics for biomarker profiling
WELLCOME TRUST (UK)
July 2006 - August 2006
A convergent strategy for high efficiency quantitative proteomics.
ENGINEERING & PHYSICAL SCIENCES RESEARCH COUNCIL
October 2005 - September 2008
Application of field programmable gate arrays to eliminate bottlenecks in near-instrument proteomics.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
November 2004 - November 2007
Development of an assay to detect subclinical ragwort poisoning in horses.
THE HORSE TRUST (UK)
January 2004 - December 2007
Between animal and between farm transmission: the role of behaviour, signalling, host genetics and management (Pathogen transmission).
DEPARTMENT FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS (UK)
April 2004 - December 2009
Global quantification of the yeast proteome
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
August 2009 - December 2014
Definition of the Skeletal Muscle 'Degradome' - Metabolic Turnover of the Proteome.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
June 2002 - January 2005
Analysis of peptides and proteins using liquid-chromatography linked to electrospray ionisation time of flight mass spectrometry.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
January 2001 - January 2004
The communication of complex information in rodent scent marks through proteins and low molecular weight components.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
January 2001 - April 2004
Ratting on rats - who, what, where and when?
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
June 2004 - May 2005
Application of quantitative proteomics to accelerate vaccine development against Schistosomiasis, a neglected tropical disease
DEPARTMENT FOR BUSINESS, INNOVATION AND SKILLS (UK)
January 2015 - December 2015
Characterization of the endothelial cell surface molecule(s) responsible for galectin-3-induced secretion of metastasis-promoting cytokines
NORTH WEST CANCER RESEARCH FUND, NORTH WEST CANCER RESEARCH INCORPORATING CLATTERBRIDGE CANCER RESEARCH (UK)
July 2014 - March 2017
Chromatography-free proteomics: a 'nextgen' perspective
ROYAL SOCIETY (CHARITABLE)
January 2012 - December 2014
Composition, heterogeneity and dynamics of the eukaryotic translation machinery
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
October 2008 - December 2011
Extending absolute quantification to the proteome-wide scale.
BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL
March 2005 - September 2008
New approaches to quantitative proteomics: application to chemical communication
WATERS CORPORATION (UK)
October 2011 - September 2015
A new approach to proteomics using binders and top-down mass spectrometry
AVACTA GROUP PLC (UK)
October 2013 - September 2017
Research collaborations
Prof J McCarthy
The University of Manchester
Collaboration on proteomics of eukaryotic translatosome
Dr Philip Whitfield
Prof Jane Hurst
Dr SImon Hubbard
The University of Manchester
Prof Simon Gaskell
The University of Manchester