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2024

Type III-B CRISPR-Cas cascade of proteolytic cleavages.

Steens, J. A., Bravo, J. P. K., Salazar, C. R. P., Yildiz, C., Amieiro, A. M., Köstlbacher, S., . . . Staals, R. H. J. (2024). Type III-B CRISPR-Cas cascade of proteolytic cleavages.. Science (New York, N.Y.), 383(6682), 512-519. doi:10.1126/science.adk0378

DOI
10.1126/science.adk0378
Journal article

2023

Late Breaking Abstract - Evolution of epithelial-mesenchymal cell circuits in the progression of pulmonary fibrosis

De Sadeleer, L., Chen, Y., Jentzsch, C., Wang, Z., Qazi, N., Lang, N., . . . Schiller, H. (2023). Late Breaking Abstract - Evolution of epithelial-mesenchymal cell circuits in the progression of pulmonary fibrosis. In Airway cell biology and immunopathology (pp. OA772). European Respiratory Society. doi:10.1183/13993003.congress-2023.oa772

DOI
10.1183/13993003.congress-2023.oa772
Conference Paper

2022

2021

Structure of the human signal peptidase complex reveals the determinants for signal peptide cleavage.

Liaci, A. M., Steigenberger, B., Telles de Souza, P. C., Tamara, S., Gröllers-Mulderij, M., Ogrissek, P., . . . Förster, F. (2021). Structure of the human signal peptidase complex reveals the determinants for signal peptide cleavage.. Molecular cell, 81(19), 3934-3948.e11. doi:10.1016/j.molcel.2021.07.031

DOI
10.1016/j.molcel.2021.07.031
Journal article

Notch-Jagged signaling complex defined by an interaction mosaic.

Zeronian, M. R., Klykov, O., Portell I de Montserrat, J., Konijnenberg, M. J., Gaur, A., Scheltema, R. A., & Janssen, B. J. C. (2021). Notch-Jagged signaling complex defined by an interaction mosaic.. Proceedings of the National Academy of Sciences of the United States of America, 118(30), e2102502118. doi:10.1073/pnas.2102502118

DOI
10.1073/pnas.2102502118
Journal article

2020

Structural Proteomics Applied to Plant Membrane Protein Complexes.

Albanese, P., Tamara, S., Scheltema, R. A., & Pagliano, C. (2020). Structural Proteomics Applied to Plant Membrane Protein Complexes.. Trends in plant science, 25(9), 945-946. doi:10.1016/j.tplants.2020.04.002

DOI
10.1016/j.tplants.2020.04.002
Journal article

To Cleave or Not To Cleave in XL-MS?

Steigenberger, B., Albanese, P., Heck, A. J. R., & Scheltema, R. A. (2020). To Cleave or Not To Cleave in XL-MS?. Journal of the American Society for Mass Spectrometry, 31(2), 196-206. doi:10.1021/jasms.9b00085

DOI
10.1021/jasms.9b00085
Journal article

Missing regions within the molecular architecture of human fibrin clots structurally resolved by XL-MS and integrative structural modeling.

Klykov, O., van der Zwaan, C., Heck, A. J. R., Meijer, A. B., & Scheltema, R. A. (2020). Missing regions within the molecular architecture of human fibrin clots structurally resolved by XL-MS and integrative structural modeling.. Proceedings of the National Academy of Sciences of the United States of America, 117(4), 1976-1987. doi:10.1073/pnas.1911785117

DOI
10.1073/pnas.1911785117
Journal article

2019

Finding and using diagnostic ions in collision induced crosslinked peptide fragmentation spectra

Steigenberger, B., Schiller, H. B., Pieters, R. J., & Scheltema, R. A. (2019). Finding and using diagnostic ions in collision induced crosslinked peptide fragmentation spectra. International Journal of Mass Spectrometry, 444, 116184. doi:10.1016/j.ijms.2019.116184

DOI
10.1016/j.ijms.2019.116184
Journal article

A Colorful Pallet of B-Phycoerythrin Proteoforms Exposed by a Multimodal Mass Spectrometry Approach

Tamara, S., Hoek, M., Scheltema, R. A., Leney, A. C., & Heck, A. J. R. (2019). A Colorful Pallet of B-Phycoerythrin Proteoforms Exposed by a Multimodal Mass Spectrometry Approach. Chem, 5(5), 1302-1317. doi:10.1016/j.chempr.2019.03.006

DOI
10.1016/j.chempr.2019.03.006
Journal article

2018

Efficient and robust proteome-wide approaches for cross-linking mass spectrometry.

Klykov, O., Steigenberger, B., Pektaş, S., Fasci, D., Heck, A. J. R., & Scheltema, R. A. (2018). Efficient and robust proteome-wide approaches for cross-linking mass spectrometry.. Nature protocols, 13(12), 2964-2990. doi:10.1038/s41596-018-0074-x

DOI
10.1038/s41596-018-0074-x
Journal article

Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca<sup>2+</sup>/CaM Controls DCV Binding and Liprin-α/TANC2 Recruits DCVs to Postsynaptic Sites.

Stucchi, R., Plucińska, G., Hummel, J. J. A., Zahavi, E. E., Guerra San Juan, I., Klykov, O., . . . Hoogenraad, C. C. (2018). Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca<sup>2+</sup>/CaM Controls DCV Binding and Liprin-α/TANC2 Recruits DCVs to Postsynaptic Sites.. Cell reports, 24(3), 685-700. doi:10.1016/j.celrep.2018.06.071

DOI
10.1016/j.celrep.2018.06.071
Journal article

2017

Phosphate Transfer in Activated Protein Complexes Reveals Interaction Sites.

Tamara, S., Scheltema, R. A., Heck, A. J. R., & Leney, A. C. (2017). Phosphate Transfer in Activated Protein Complexes Reveals Interaction Sites.. Angewandte Chemie (International ed. in English), 56(44), 13641-13644. doi:10.1002/anie.201706749

DOI
10.1002/anie.201706749
Journal article

Combining Deep Sequencing, Proteomics, Phosphoproteomics, and Functional Screens To Discover Novel Regulators of Sphingolipid Homeostasis.

Lebesgue, N., Megyeri, M., Cristobal, A., Scholten, A., Chuartzman, S. G., Voichek, Y., . . . Lemeer, S. (2017). Combining Deep Sequencing, Proteomics, Phosphoproteomics, and Functional Screens To Discover Novel Regulators of Sphingolipid Homeostasis.. Journal of proteome research, 16(2), 571-582. doi:10.1021/acs.jproteome.6b00691

DOI
10.1021/acs.jproteome.6b00691
Journal article

Opposite Electron-Transfer Dissociation and Higher-Energy Collisional Dissociation Fragmentation Characteristics of Proteolytic K/R(X)<sub>n</sub> and (X)<sub>n</sub>K/R Peptides Provide Benefits for Peptide Sequencing in Proteomics and Phosphoproteomics.

Tsiatsiani, L., Giansanti, P., Scheltema, R. A., van den Toorn, H., Overall, C. M., Altelaar, A. F. M., & Heck, A. J. R. (2017). Opposite Electron-Transfer Dissociation and Higher-Energy Collisional Dissociation Fragmentation Characteristics of Proteolytic K/R(X)<sub>n</sub> and (X)<sub>n</sub>K/R Peptides Provide Benefits for Peptide Sequencing in Proteomics and Phosphoproteomics.. Journal of proteome research, 16(2), 852-861. doi:10.1021/acs.jproteome.6b00825

DOI
10.1021/acs.jproteome.6b00825
Journal article

Arginine (Di)methylated Human Leukocyte Antigen Class I Peptides Are Favorably Presented by HLA-B*07.

Marino, F., Mommen, G. P. M., Jeko, A., Meiring, H. D., van Gaans-van den Brink, J. A. M., Scheltema, R. A., . . . Heck, A. J. R. (2017). Arginine (Di)methylated Human Leukocyte Antigen Class I Peptides Are Favorably Presented by HLA-B*07.. Journal of proteome research, 16(1), 34-44. doi:10.1021/acs.jproteome.6b00528

DOI
10.1021/acs.jproteome.6b00528
Journal article

2016

Deciphering the Interplay among Multisite Phosphorylation, Interaction Dynamics, and Conformational Transitions in a Tripartite Protein System.

Lössl, P., Brunner, A. M., Liu, F., Leney, A. C., Yamashita, M., Scheltema, R. A., & Heck, A. J. R. (2016). Deciphering the Interplay among Multisite Phosphorylation, Interaction Dynamics, and Conformational Transitions in a Tripartite Protein System.. ACS central science, 2(7), 445-455. doi:10.1021/acscentsci.6b00053

DOI
10.1021/acscentsci.6b00053
Journal article

Implementation of Ultraviolet Photodissociation on a Benchtop Q Exactive Mass Spectrometer and Its Application to Phosphoproteomics.

Fort, K. L., Dyachenko, A., Potel, C. M., Corradini, E., Marino, F., Barendregt, A., . . . Heck, A. J. R. (2016). Implementation of Ultraviolet Photodissociation on a Benchtop Q Exactive Mass Spectrometer and Its Application to Phosphoproteomics.. Analytical chemistry, 88(4), 2303-2310. doi:10.1021/acs.analchem.5b04162

DOI
10.1021/acs.analchem.5b04162
Journal article

2015

2014

The Q Exactive HF, a Benchtop mass spectrometer with a pre-filter, high-performance quadrupole and an ultra-high-field Orbitrap analyzer.

Scheltema, R. A., Hauschild, J. -P., Lange, O., Hornburg, D., Denisov, E., Damoc, E., . . . Mann, M. (2014). The Q Exactive HF, a Benchtop mass spectrometer with a pre-filter, high-performance quadrupole and an ultra-high-field Orbitrap analyzer.. Molecular & cellular proteomics : MCP, 13(12), 3698-3708. doi:10.1074/mcp.m114.043489

DOI
10.1074/mcp.m114.043489
Journal article

Structural model of a CRISPR RNA-silencing complex reveals the RNA-target cleavage activity in Cmr4.

Benda, C., Ebert, J., Scheltema, R. A., Schiller, H. B., Baumgärtner, M., Bonneau, F., . . . Conti, E. (2014). Structural model of a CRISPR RNA-silencing complex reveals the RNA-target cleavage activity in Cmr4.. Molecular cell, 56(1), 43-54. doi:10.1016/j.molcel.2014.09.002

DOI
10.1016/j.molcel.2014.09.002
Journal article

2013

High performance computational analysis of large-scale proteome data sets to assess incremental contribution to coverage of the human genome.

Neuhauser, N., Nagaraj, N., McHardy, P., Zanivan, S., Scheltema, R., Cox, J., & Mann, M. (2013). High performance computational analysis of large-scale proteome data sets to assess incremental contribution to coverage of the human genome.. Journal of proteome research, 12(6), 2858-2868. doi:10.1021/pr400181q

DOI
10.1021/pr400181q
Journal article

Direct proteomic quantification of the secretome of activated immune cells.

Meissner, F., Scheltema, R. A., Mollenkopf, H. -J., & Mann, M. (2013). Direct proteomic quantification of the secretome of activated immune cells.. Science (New York, N.Y.), 340(6131), 475-478. doi:10.1126/science.1232578

DOI
10.1126/science.1232578
Journal article

2012

SprayQc: a real-time LC-MS/MS quality monitoring system to maximize uptime using off the shelf components.

Scheltema, R. A., & Mann, M. (2012). SprayQc: a real-time LC-MS/MS quality monitoring system to maximize uptime using off the shelf components.. Journal of proteome research, 11(6), 3458-3466. doi:10.1021/pr201219e

DOI
10.1021/pr201219e
Journal article

2011

Bayesian Approaches for Mass Spectrometry‐Based Metabolomics

Rogers, S., Scheltema, R. A., Barrett, M., & Breitling, R. (2011). Bayesian Approaches for Mass Spectrometry‐Based Metabolomics. In Unknown Book (pp. 467-476). Wiley. doi:10.1002/9781119970606.ch24

DOI
10.1002/9781119970606.ch24
Chapter

Richard A Scheltema.

Scheltema, R. A. (2011). Richard A Scheltema.. Bioanalysis, 3(11), 1197-1199. doi:10.4155/bio.11.96

DOI
10.4155/bio.11.96
Journal article

Andromeda: a peptide search engine integrated into the MaxQuant environment.

Cox, J., Neuhauser, N., Michalski, A., Scheltema, R. A., Olsen, J. V., & Mann, M. (2011). Andromeda: a peptide search engine integrated into the MaxQuant environment.. Journal of proteome research, 10(4), 1794-1805. doi:10.1021/pr101065j

DOI
10.1021/pr101065j
Journal article

Erratum to: Towards an unbiased metabolic profiling of protozoan parasites: optimisation of a Leishmania sampling protocol for HILIC-orbitrap analysis

t’Kindt, R., Jankevics, A., Scheltema, R. A., Zheng, L., Watson, D. G., Dujardin, J. -C., . . . Decuypere, S. (2011). Erratum to: Towards an unbiased metabolic profiling of protozoan parasites: optimisation of a Leishmania sampling protocol for HILIC-orbitrap analysis. Analytical and Bioanalytical Chemistry, 400(2), 635. doi:10.1007/s00216-011-4796-7

DOI
10.1007/s00216-011-4796-7
Journal article

PeakML/mzMatch: a file format, Java library, R library, and tool-chain for mass spectrometry data analysis.

Scheltema, R. A., Jankevics, A., Jansen, R. C., Swertz, M. A., & Breitling, R. (2011). PeakML/mzMatch: a file format, Java library, R library, and tool-chain for mass spectrometry data analysis.. Analytical chemistry, 83(7), 2786-2793. doi:10.1021/ac2000994

DOI
10.1021/ac2000994
Journal article

2010

Two time-point assessment of bile acid kinetics in humans using stable isotopes.

Stellaard, F., Bloks, V. W., Burgerhof, H. G. M., Scheltema, R. A., Murphy, E. J., Romijn, H. A., . . . Kuipers, F. (2010). Two time-point assessment of bile acid kinetics in humans using stable isotopes.. Isotopes in environmental and health studies, 46(3), 325-336. doi:10.1080/10256016.2010.503894

DOI
10.1080/10256016.2010.503894
Journal article

The potential of metabolomics for Leishmania research in the post-genomics era.

Scheltema, R. A., Decuypere, S., T'kindt, R., Dujardin, J. -C., Coombs, G. H., & Breitling, R. (2010). The potential of metabolomics for Leishmania research in the post-genomics era.. Parasitology, 137(9), 1291-1302. doi:10.1017/s0031182009992022

DOI
10.1017/s0031182009992022
Journal article

XGAP: a uniform and extensible data model and software platform for genotype and phenotype experiments

Swertz, M. A., van der Velde, K. J., Tesson, B. M., Scheltema, R. A., Arends, D., Vera, G., . . . Jansen, R. C. (2010). XGAP: a uniform and extensible data model and software platform for genotype and phenotype experiments. GENOME BIOLOGY, 11(3). doi:10.1186/gb-2010-11-3-r27

DOI
10.1186/gb-2010-11-3-r27
Journal article

Towards an unbiased metabolic profiling of protozoan parasites: optimisation of a Leishmania sampling protocol for HILIC-orbitrap analysis.

t'Kindt, R., Jankevics, A., Scheltema, R. A., Zheng, L., Watson, D. G., Dujardin, J. -C., . . . Decuypere, S. (2010). Towards an unbiased metabolic profiling of protozoan parasites: optimisation of a Leishmania sampling protocol for HILIC-orbitrap analysis.. Analytical and bioanalytical chemistry, 398(5), 2059-2069. doi:10.1007/s00216-010-4139-0

DOI
10.1007/s00216-010-4139-0
Journal article

2009

Probabilistic assignment of formulas to mass peaks in metabolomics experiments.

Rogers, S., Scheltema, R. A., Girolami, M., & Breitling, R. (2009). Probabilistic assignment of formulas to mass peaks in metabolomics experiments.. Bioinformatics (Oxford, England), 25(4), 512-518. doi:10.1093/bioinformatics/btn642

DOI
10.1093/bioinformatics/btn642
Journal article

System-wide molecular evidence for phenotypic buffering in Arabidopsis.

Fu, J., Keurentjes, J. J. B., Bouwmeester, H., America, T., Verstappen, F. W. A., Ward, J. L., . . . Jansen, R. C. (2009). System-wide molecular evidence for phenotypic buffering in Arabidopsis.. Nature genetics, 41(2), 166-167. doi:10.1038/ng.308

DOI
10.1038/ng.308
Journal article

Simple data-reduction method for high-resolution LC-MS data in metabolomics.

Scheltema, R., Decuypere, S., Dujardin, J., Watson, D., Jansen, R., & Breitling, R. (2009). Simple data-reduction method for high-resolution LC-MS data in metabolomics.. Bioanalysis, 1(9), 1551-1557. doi:10.4155/bio.09.146

DOI
10.4155/bio.09.146
Journal article

2008

Increasing the mass accuracy of high-resolution LC-MS data using background ions: a case study on the LTQ-Orbitrap.

Scheltema, R. A., Kamleh, A., Wildridge, D., Ebikeme, C., Watson, D. G., Barrett, M. P., . . . Breitling, R. (2008). Increasing the mass accuracy of high-resolution LC-MS data using background ions: a case study on the LTQ-Orbitrap.. Proteomics, 8(22), 4647-4656. doi:10.1002/pmic.200800314

DOI
10.1002/pmic.200800314
Journal article

Metabolomic profiling using Orbitrap Fourier transform mass spectrometry with hydrophilic interaction chromatography: a method with wide applicability to analysis of biomolecules.

Kamleh, A., Barrett, M. P., Wildridge, D., Burchmore, R. J. S., Scheltema, R. A., & Watson, D. G. (2008). Metabolomic profiling using Orbitrap Fourier transform mass spectrometry with hydrophilic interaction chromatography: a method with wide applicability to analysis of biomolecules.. Rapid communications in mass spectrometry : RCM, 22(12), 1912-1918. doi:10.1002/rcm.3564

DOI
10.1002/rcm.3564
Journal article