Publications
Selected publications
- Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi (Journal article - 2023)
- High-speed imaging of glutamate release with genetically encoded sensors (Journal article - 2019)
- Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses (Journal article - 2018)
- Biophysical and functional characterization of hippocalcin mutants responsible for human dystonia (Journal article - 2017)
- Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics (Journal article - 2016)
2024
Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome
Barker, E., Milburn, A., Helassa, N., Hammond, D., Sanchez-Soriano, N., Morgan, A., & Barclay, J. (2024). Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome.. The Biochemical journal, BCJ20230319. doi:10.1042/bcj20230319
2023
Long QT syndrome‐associated calmodulin variants disrupt the activity of the slowly activating delayed rectifier potassium channel
McCormick, L., Wadmore, K., Milburn, A., Gupta, N., Morris, R., Held, M., . . . Helassa, N. (2023). Long QT syndrome-associated calmodulin variants disrupt the activity of the slow delayed rectifier potassium current. JOURNAL OF PHYSIOLOGY-LONDON, 601(17), 3739-3764. doi:10.1113/JP284994
Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi
Yaganoglu, S., Kalyviotis, K., Vagena-Pantoula, C., Julich, D., Gaub, B. M., Welling, M., . . . Pantazis, P. (2023). Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi. NATURE COMMUNICATIONS, 14(1). doi:10.1038/s41467-023-40134-y
Calmodulin variant E140G associated with long QT syndrome impairs CaMKIIδ autophosphorylation and L-type calcium channel inactivation
Prakash, O., Gupta, N., Milburn, A., McCormick, L., Deugi, V., Fisch, P., . . . Helassa, N. (2023). Calmodulin variant E140G associated with long QT syndrome impairs CaMKIIδ autophosphorylation and L-type calcium channel inactivation. JOURNAL OF BIOLOGICAL CHEMISTRY, 299(1). doi:10.1016/j.jbc.2022.102777
Editorial: The role of calcium and calcium binding proteins in cell physiology and disease
Thomas, N. L., Dart, C., & Helassa, N. (2023). Editorial: The role of calcium and calcium binding proteins in cell physiology and disease. FRONTIERS IN PHYSIOLOGY, 14. doi:10.3389/fphys.2023.1228885
2022
Lysosome exocytosis is required for mitosis in mammalian cells
Nugues, C., Rajamanoharan, D., Burgoyne, R. D., Haynes, L. P., & Helassa, N. (2022). Lysosome exocytosis is required for mitosis in mammalian cells. Biochemical and Biophysical Research Communications, 626, 211-219. doi:10.1016/j.bbrc.2022.08.024
Vesicular release probability sets the strength of individual Schaffer collateral synapses
Duerst, C. D., Wiegert, J. S., Schulze, C., Helassa, N., Toeroek, K., & Oertner, T. G. (2022). Vesicular release probability sets the strength of individual Schaffer collateral synapses. NATURE COMMUNICATIONS, 13(1). doi:10.1038/s41467-022-33565-6
Disease-associated calmodulin mutations disrupt L-type Ca2+channel (Cav1.2) activity and CaMKIIdelta phosphorylation in long QT syndrome
Helassa, N., Prakash, O., Gupta, N., McCormick, L. F., Antonyuk, S., & Dart, C. (2022). Disease-associated calmodulin mutations disrupt L-type Ca2+channel (Cav1.2) activity and CaMKIIdelta phosphorylation in long QT syndrome. In EUROPEAN HEART JOURNAL Vol. 43 (pp. 2982). Retrieved from https://www.webofscience.com/
Long QT syndrome-associated calmodulin mutations and their interactions with the Kv7.1 voltage-gated potassium channel
McCormick, L. F., Gupta, N., Prakash, O., Lian, L. Y., Dart, C., & Helassa, N. (2022). Long QT syndrome-associated calmodulin mutations and their interactions with the Kv7.1 voltage-gated potassium channel. European Heart Journal, 43(Supplement_2). doi:10.1093/eurheartj/ehac544.2983
Long QT Syndrome-Associated Mutations D130V and E141K Affect the Structure-Function Relationship of Calmodulin
Wadmore, K., Dart, C., & Helassa, N. (2022). Long QT Syndrome-Associated Mutations D130V and E141K Affect the Structure-Function Relationship of Calmodulin. In ACTA PHYSIOLOGICA Vol. 236 (pp. 586-587). Retrieved from https://www.webofscience.com/
Mitosis, Focus on Calcium
Nugues, C., Helassa, N., & Haynes, L. P. (2022). Mitosis, Focus on Calcium. FRONTIERS IN PHYSIOLOGY, 13. doi:10.3389/fphys.2022.951979
CALMODULIN MUTATIONS ASSOCIATED WITH LONG QT SYNDROME IMPAIR L-TYPE CALCIUM CHANNEL REGULATION
Gupta, N., McCormick, L. F., Prakash, O., Lian, L. -Y., Dart, C., & Helassa, N. (2022). CALMODULIN MUTATIONS ASSOCIATED WITH LONG QT SYNDROME IMPAIR L-TYPE CALCIUM CHANNEL REGULATION. In HEART Vol. 108 (pp. A150-A151). doi:10.1136/heartjnl-2022-BCS.192
LONG QT SYNDROME-ASSOCIATED CALMODULIN MUTATIONS AFFECT INTERACTION WITH L-TYPE CA2+CHANNEL (CAV1.2) AND CAMKII Delta ACTIVITY
Prakash, O., Gupta, N., McCormick, L. F., Antonyuk, S., Dart, C., & Helassa, N. (2022). LONG QT SYNDROME-ASSOCIATED CALMODULIN MUTATIONS AFFECT INTERACTION WITH L-TYPE CA2+CHANNEL (CAV1.2) AND CAMKIIΔ ACTIVITY. In HEART Vol. 108 (pp. A148). doi:10.1136/heartjnl-2022-BCS.189
LONG QT SYNDROME-ASSOCIATED CALMODULIN MUTATIONS DISRUPT KV7.1 VOLTAGE-GATED POTASSIUM CHANNEL FUNCTION
McCormick, L. F., Gupta, N., Prakash, O., Lian, L. -Y., Dart, C., & Helassa, N. (2022). LONG QT SYNDROME-ASSOCIATED CALMODULIN MUTATIONS DISRUPT KV7.1 VOLTAGE-GATED POTASSIUM CHANNEL FUNCTION. In HEART Vol. 108 (pp. A149). doi:10.1136/heartjnl-2022-BCS.190
CPVT-associated calmodulin variants N53I and A102V dysregulate calcium signalling via different mechanisms
Prakash, O., Held, M., McCormick, L. F., Gupta, N., Lian, L. -Y., Antonyuk, S., . . . Helassa, N. (2022). CPVT-associated calmodulin variants N531 and A102V dysregulate Ca<SUP>2+</SUP> signalling via different mechanisms. JOURNAL OF CELL SCIENCE, 135(2). doi:10.1242/jcs.258796
2021
TSPAN‐7 as a key regulator of glucose‐stimulated Ca2+ influx and insulin secretion
Helassa, N. (2021). TSPAN-7 as a key regulator of glucose-stimulated Ca<SUP>2+</SUP> influx and insulin secretion. JOURNAL OF PHYSIOLOGY-LONDON, 599(6), 1733-1734. doi:10.1113/JP281053
2020
LQTS-Associated Mutants of Calmodulin Show Disrupted Interaction with L-Type Calcium Channels
Gupta, N., McCormick, L. F., Haynes, L. P., Dart, C., & Helassa, N. (2020). LQTS-Associated Mutants of Calmodulin Show Disrupted Interaction with L-Type Calcium Channels. In BIOPHYSICAL JOURNAL Vol. 118 (pp. 405A). Retrieved from https://www.webofscience.com/
Long-QT Syndrome-Associated Calmodulin Mutations and Their Interactions at the K<sub>v</sub>7.1 Potassium Channel
McCormick, L. F., Gupta, N., Haynes, L. P., Antonyuk, S., Dart, C., & Helassa, N. (2020). Long-QT Syndrome-Associated Calmodulin Mutations and Their Interactions at the K<sub>v</sub>7.1 Potassium Channel. In BIOPHYSICAL JOURNAL Vol. 118 (pp. 404A). Retrieved from https://www.webofscience.com/
Kinetic Mechanisms of Fast Glutamate Sensing by Fluorescent Protein Probes
Coates, C., Kerruth, S., Helassa, N., & Torok, K. (2020). Kinetic Mechanisms of Fast Glutamate Sensing by Fluorescent Protein Probes. BIOPHYSICAL JOURNAL, 118(1), 117-127. doi:10.1016/j.bpj.2019.11.006
2019
A centrosome‐localized calcium signal is essential for mammalian cell mitosis
Helassa, N., Nugues, C., Rajamanoharan, D., Burgoyne, R. D., & Haynes, L. P. (2019). A centrosome-localized calcium signal is essential for mammalian cell mitosis. The FASEB Journal, 33(12), 14602-14610. doi:10.1096/fj.201901662R
Single Synapse Indicators of Impaired Glutamate Clearance Derived from Fast iGluu Imaging of Cortical Afferents in the Striatum of Normal and Huntington (Q175) Mice
Dvorzhak, A., Helassa, N., Torok, K., Schmitz, D., & Grantyn, R. (2019). Single Synapse Indicators of Impaired Glutamate Clearance Derived from Fast iGluu Imaging of Cortical Afferents in the Striatum of Normal and Huntington (Q175) Mice. Journal of Neuroscience, 39(20), 3970-3982. doi:10.1523/JNEUROSCI.2865-18.2019
Calcium Sensors in Neuronal Function and Dysfunction.
Burgoyne, R. D., Helassa, N., McCue, H. V., & Haynes, L. P. (2019). Calcium Sensors in Neuronal Function and Dysfunction. COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 11(5). doi:10.1101/cshperspect.a035154
High-speed imaging of glutamate release with genetically encoded sensors
Duerst, C. D., Wiegert, J. S., Helassa, N., Kerruth, S., Coates, C., Schulze, C., . . . Oertner, T. G. (2019). High-speed imaging of glutamate release with genetically encoded sensors. NATURE PROTOCOLS, 14(5), 1401-1424. doi:10.1038/s41596-019-0143-9
2018
Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses
Helassa, N., Dürst, C. D., Coates, C., Kerruth, S., Arif, U., Schulze, C., . . . Török, K. (2018). Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses.. Proceedings of the National Academy of Sciences of the United States of America, 115(21), 5594-5599. doi:10.1073/pnas.1720648115
Dystonia-Associated Hippocalcin Mutants Dysregulate Cellular Calcium Influx
Helassa, N., Antonyuk, S. V., Lian, L. -Y., Haynes, L. P., & Burgoyne, R. D. (2018). Dystonia-Associated Hippocalcin Mutants Dysregulate Cellular Calcium Influx. In BIOPHYSICAL JOURNAL Vol. 114 (pp. 467A-468A). Retrieved from https://www.webofscience.com/
Novel iGluSnFR Variants Optimised for Rapid Glutamate Imaging
Helassa, N., Duerst, C., Coates, C., Arif, U., Schulze, C., Wiegert, S., . . . Torok, K. (2018). Novel iGluSnFR Variants Optimised for Rapid Glutamate Imaging. In BIOPHYSICAL JOURNAL Vol. 114 (pp. 153A). Retrieved from http://gateway.webofknowledge.com/
Diffusion-Limited Glutamate Binding to GluBP is Revealed by Novel Fluorescent Probe
Coates, C., Helassa, N., & Torok, K. (2018). Diffusion-Limited Glutamate Binding to GluBP is Revealed by Novel Fluorescent Probe. In BIOPHYSICAL JOURNAL Vol. 114 (pp. 153A). doi:10.1016/j.bpj.2017.11.859
2017
Biophysical and functional characterization of hippocalcin mutants responsible for human dystonia
Helassa, N., Antonyuk, S. V., Lian, L. -Y., Haynes, L. P., & Burgoyne, R. D. (2017). Biophysical and functional characterization of hippocalcin mutants responsible for human dystonia. Human molecular genetics, 26(13), 2426-2435. doi:10.1093/hmg/ddx133
2016
Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics
Helassa, N., Podor, B., Fine, A., & Török, K. (2016). Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics. Scientific Reports, 6. doi:10.1038/srep38276
Development of Fast-Response GCaMP6 Calcium Sensors for Monitoring Neuronal Action Potential
Helassa, N., Podor, B., Fine, A., & Torok, K. (2016). Development of Fast-Response GCaMP6 Calcium Sensors for Monitoring Neuronal Action Potential. In BIOPHYSICAL JOURNAL Vol. 110 (pp. 372A). doi:10.1016/j.bpj.2015.11.2005
2015
Fast-Response Calmodulin-Based Fluorescent Indicators Reveal Rapid Intracellular Calcium Dynamics
Helassa, N., Zhang, X. -H., Conte, I., Scaringi, J., Esposito, E., Bradley, J., . . . Török, K. (2015). Fast-Response Calmodulin-Based Fluorescent Indicators Reveal Rapid Intracellular Calcium Dynamics. SCIENTIFIC REPORTS, 5, 15 pages. doi:10.1038/srep15978
GCaMP3-Derived Genetically Encoded Probes as Rapid Intracellular Signals for Calcium Dynamics
Scaringi, J. A., Helassa, N., Torok, K., & Morad, M. (2015). GCaMP3-Derived Genetically Encoded Probes as Rapid Intracellular Signals for Calcium Dynamics. In BIOPHYSICAL JOURNAL Vol. 108 (pp. 150A). doi:10.1016/j.bpj.2014.11.828
2014
A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration
Helassa, N., Garnett, J. P., Farrant, M., Khan, F., Pickup, J. C., Hahn, K. M., . . . Baines, D. L. (2014). A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration. Biochemical Journal, 464(2), 213-220. doi:10.1042/BJ20141041
Ultrafast Genetically Encoded Calcium Indicators for Visualizing Calcium Flux and Action Potentials
Helassa, N., Esposito, E., Carter, T., Bradley, J., Ogden, D., & Toeroek, K. (2014). Ultrafast Genetically Encoded Calcium Indicators for Visualizing Calcium Flux and Action Potentials. In BIOPHYSICAL JOURNAL Vol. 106 (pp. 242A). doi:10.1016/j.bpj.2013.11.1418
2013
Structure, Biological Activity and Environmental Fate of Insecticidal Bt (Bacillus thuringiensis) Cry Proteins of Bacterial and Genetically Modified Plant Origin
Helassa, N., Quiquampoix, H., & Staunton, S. (2013). Structure, Biological Activity and Environmental Fate of Insecticidal Bt (Bacillus thuringiensis) Cry Proteins of Bacterial and Genetically Modified Plant Origin. In MOLECULAR ENVIRONMENTAL SOIL SCIENCE (pp. 49-77). doi:10.1007/978-94-007-4177-5_3
2012
Peptidoglycan Assembly Machines: The Biochemical Evidence
Zapun, A., Noirclerc-Savoye, M., Helassa, N., & Vernet, T. (2012). Peptidoglycan Assembly Machines: The Biochemical Evidence. MICROBIAL DRUG RESISTANCE, 18(3), 256-260. doi:10.1089/mdr.2011.0236
The membrane anchor of penicillin‐binding protein PBP2a from Streptococcus pneumoniae influences peptidoglycan chain length
Helassa, N., Vollmer, W., Breukink, E., Vernet, T., & Zapun, A. (2012). The membrane anchor of penicillin-binding protein PBP2a from Streptococcus pneumoniae influences peptidoglycan chain length. FEBS JOURNAL, 279(11), 2071-2081. doi:10.1111/j.1742-4658.2012.08592.x
2011
Adsorption on montmorillonite prevents oligomerization of Bt Cry1Aa toxin
Helassa, N., Revault, M., Quiquampoix, H., Dejardin, P., Staunton, S., & Noinville, S. (2011). Adsorption on montmorillonite prevents oligomerization of <i>Bt</i> Cry1Aa toxin. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 356(2), 718-725. doi:10.1016/j.jcis.2011.01.064
Effects of physicochemical interactions and microbial activity on the persistence of Cry1Aa Bt (<i>Bacillus thuringiensis</i>) toxin in soil
Helassa, N., M'Charek, A., Quiquampoix, H., Noinville, S., Dejardin, P., Frutos, R., & Staunton, S. (2011). Effects of physicochemical interactions and microbial activity on the persistence of Cry1Aa Bt (<i>Bacillus thuringiensis</i>) toxin in soil. SOIL BIOLOGY & BIOCHEMISTRY, 43(5), 1089-1097. doi:10.1016/j.soilbio.2011.01.030
2010
Adsorption of Alexa-Labeled Bt Toxin on Mica, Glass, and Hydrophobized Glass: Study by Normal Scanning Confocal Fluorescence
Janot, J. -M., Boissiere, M., Thami, T., Tronel-Peyroz, E., Helassa, N., Noinville, S., . . . Dejardin, P. (2010). Adsorption of Alexa-Labeled Bt Toxin on Mica, Glass, and Hydrophobized Glass: Study by Normal Scanning Confocal Fluorescence. BIOMACROMOLECULES, 11(6), 1661-1666. doi:10.1021/bm100313n
Mobility of adsorbed Cry1Aa insecticidal toxin from Bacillus thuringiensis (Bt) on montmorillonite measured by fluorescence recovery after photobleaching (FRAP)
Helassa, N., Daudin, G., Noinville, S., Janot, J. -M., Dejardin, P., Staunton, S., & Quiquampoix, H. (2010). Mobility of adsorbed Cry1Aa insecticidal toxin from Bacillus thuringiensis (Bt) on montmorillonite measured by fluorescence recovery after photobleaching (FRAP). PHILOSOPHICAL MAGAZINE, 90(17-18), 2365-2371. doi:10.1080/14786431003636071
Interaction of Bttoxin with Organo-mineral Surfaces and Consequences for Its Fate in the Environment
Helassa, N., Noinville, S., Dejardin, P., Jano, J. -M., Quiquampoix, H., & Staunton, S. (2010). Interaction of Bttoxin with Organo-mineral Surfaces and Consequences for Its Fate in the Environment. In MOLECULAR ENVIRONMENTAL SOIL SCIENCE AT THE INTERFACES IN THE EARTH’S CRITICAL ZONE (pp. 187-189). doi:10.1007/978-3-642-05297-2_56
2009
Adsorption and desorption of monomeric Bt (Bacillus thuringiensis) Cry1Aa toxin on montmorillonite and kaolinite
Helassa, N., Quiquampoix, H., Noinville, S., Szponarski, W., & Staunton, S. (2009). Adsorption and desorption of monomeric Bt (<i>Bacillus thuringiensis</i>) Cry1Aa toxin on montmorillonite and kaolinite. SOIL BIOLOGY & BIOCHEMISTRY, 41(3), 498-504. doi:10.1016/j.soilbio.2008.12.008