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2024

Moonlighting on the Fasciola hepatica tegument: Enolase, a glycolytic enzyme, interacts with the extracellular matrix and fibrinolytic system of the host

O’Kelly, E., Cwiklinski, K., De Marco Verissimo, C., Calvani, N. E. D., López Corrales, J., Jewhurst, H., . . . González-Miguel, J. (n.d.). Moonlighting on the Fasciola hepatica tegument: Enolase, a glycolytic enzyme, interacts with the extracellular matrix and fibrinolytic system of the host. PLOS Neglected Tropical Diseases, 18(8), e0012069. doi:10.1371/journal.pntd.0012069

DOI
10.1371/journal.pntd.0012069
Journal article

2023

Glycan complexity and heterogeneity of glycoproteins in somatic extracts and secretome of the infective stage of the helminth Fasciola hepatica

De Marco Verissimo, C., Cwiklinski, K., Nilsson, J., Mirgorodskaya, E., Jin, C., Karlsson, N. G., & Dalton, J. P. (2023). Glycan complexity and heterogeneity of glycoproteins in somatic extracts and secretome of the infective stage of the helminth Fasciola hepatica. Molecular and Cellular Proteomics, 100684. doi:10.1016/j.mcpro.2023.100684

DOI
10.1016/j.mcpro.2023.100684
Journal article

Fasciola hepatica antioxidant and protease-inhibitor cocktail recombinant vaccines administered five times elicit potent and sustained immune responses in sheep but do not confer protection

Cwiklinski, K., McEvoy, A., Corrales, J. L., Jewhurst, H., Calvani, N. E. D., De Marco Verissimo, C., . . . Lalor, R. (2023). Fasciola hepatica antioxidant and protease-inhibitor cocktail recombinant vaccines administered five times elicit potent and sustained immune responses in sheep but do not confer protection. Veterinary Parasitology, 110049. doi:10.1016/j.vetpar.2023.110049

DOI
10.1016/j.vetpar.2023.110049
Journal article

2022

Exploiting Comparative Omics to Understand the Pathogenic and Virulence-Associated Protease: Anti-Protease Relationships in the Zoonotic Parasites <i>Fasciola hepatica</i> and <i>Fasciola gigantica</i>

Cwiklinski, K., & Dalton, J. P. (2022). Exploiting Comparative Omics to Understand the Pathogenic and Virulence-Associated Protease: Anti-Protease Relationships in the Zoonotic Parasites <i>Fasciola hepatica</i> and <i>Fasciola gigantica</i>. GENES, 13(10). doi:10.3390/genes13101854

DOI
10.3390/genes13101854
Journal article

Two Distinct Superoxidase Dismutases (SOD) Secreted by the Helminth Parasite <i>Fasciola hepatica</i> Play Roles in Defence against Metabolic and Host Immune Cell-Derived Reactive Oxygen Species (ROS) during Growth and Development

Calvani, N. E. D., Verissimo, C. D. M., Jewhurst, H. L., Cwiklinski, K., Flaus, A., & Dalton, J. P. (2022). Two Distinct Superoxidase Dismutases (SOD) Secreted by the Helminth Parasite <i>Fasciola hepatica</i> Play Roles in Defence against Metabolic and Host Immune Cell-Derived Reactive Oxygen Species (ROS) during Growth and Development. ANTIOXIDANTS, 11(10). doi:10.3390/antiox11101968

DOI
10.3390/antiox11101968
Journal article

Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-Like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system

De Marco Verissimo, C., López Corrales, J., Dorey, A. L., Cwiklinski, K., Lalor, R., Calvani, N. E. D., . . . Dalton, J. P. (2022). Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system.. Epidemiology and infection, 150, e128. doi:10.1017/s0950268822001078

DOI
10.1017/s0950268822001078
Journal article

Stage-specific miRNAs regulate gene expression associated with growth, development and parasite-host interaction during the intra-mammalian migration of the zoonotic helminth parasite Fasciola hepatica

Ricafrente, A., Cwiklinski, K., Nguyen, H., Dalton, J. P., Tran, N., & Donnelly, S. (2022). Stage-specific miRNAs regulate gene expression associated with growth, development and parasite-host interaction during the intra-mammalian migration of the zoonotic helminth parasite Fasciola hepatica. BMC Genomics, 23(1). doi:10.1186/s12864-022-08644-z

DOI
10.1186/s12864-022-08644-z
Journal article

Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins

De Marco Verissimo, C., Jewhurst, H. L., Dobó, J., Gál, P., Dalton, J. P., & Cwiklinski, K. (n.d.). Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins. PLOS Pathogens, 18(1), e1010226. doi:10.1371/journal.ppat.1010226

DOI
10.1371/journal.ppat.1010226
Journal article

2021

Pathogenicity and virulence of the liver flukes Fasciola hepatica and Fasciola Gigantica that cause the zoonosis Fasciolosis

Lalor, R., Cwiklinski, K., Calvani, N. E. D., Dorey, A., Hamon, S., Corrales, J. L., . . . De Marco Verissimo, C. (2021). Pathogenicity and virulence of the liver flukes <i>Fasciola hepatica</i> and <i>Fasciola</i><i>Gigantica</i> that cause the zoonosis Fasciolosis.. Virulence, 12(1), 2839-2867. doi:10.1080/21505594.2021.1996520

DOI
10.1080/21505594.2021.1996520
Journal article

The discovery of <i>Fasciola hepatica</i> and its life cycle.

Andrews, S. J., Cwiklinski, K., & Dalton, J. P. (2021). The discovery of <i>Fasciola hepatica</i> and its life cycle.. In Fasciolosis (pp. 1-22). CABI. doi:10.1079/9781789246162.0001

DOI
10.1079/9781789246162.0001
Chapter

Applying 'omics' technologies to understand <i>Fasciola</i> spp. biology.

Cwiklinski, K., Verissimo, C. D. M., McVeigh, P., Donnelly, S., & Dalton, J. P. (2021). Applying 'omics' technologies to understand <i>Fasciola</i> spp. biology.. In Fasciolosis (pp. 338-378). CABI. doi:10.1079/9781789246162.0011

DOI
10.1079/9781789246162.0011
Chapter

The Zoonotic Helminth Parasite Fasciola hepatica: Virulence-Associated Cathepsin B and Cathepsin L Cysteine Peptidases Secreted by Infective Newly Excysted Juveniles (NEJ)

Barbour, T., Cwiklinski, K., Lalor, R., Dalton, J. P., & De Marco Verissimo, C. (2021). The Zoonotic Helminth Parasite <i>Fasciola hepatica</i><i>:</i> Virulence-Associated Cathepsin B and Cathepsin L Cysteine Peptidases Secreted by Infective Newly Excysted Juveniles (NEJ).. Animals : an open access journal from MDPI, 11(12), 3495. doi:10.3390/ani11123495

DOI
10.3390/ani11123495
Journal article

Antigen-specific response of CD4+ T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep

Pérez-Caballero, R., Martínez-Moreno, F. J., Corripio-Miyar, Y., McNeilly, T. N., Cwiklinski, K., Dalton, J. P., . . . Buffoni, L. (2021). Antigen-specific response of CD4+ T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep. Veterinary Research, 52(1). doi:10.1186/s13567-021-00963-5

DOI
10.1186/s13567-021-00963-5
Journal article

Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics

Becerro-Recio, D., González-Miguel, J., Ucero, A., Sotillo, J., Martínez-Moreno, Á., Pérez-Arévalo, J., . . . Siles-Lucas, M. (2021). Recognition Pattern of the <i>Fasciola hepatica</i> Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics.. Pathogens (Basel, Switzerland), 10(6), 725. doi:10.3390/pathogens10060725

DOI
10.3390/pathogens10060725
Journal article

Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (PfM1AAP) and M17 leucyl aminopeptidase (PfM17LAP)

Mathew, R., Wunderlich, J., Thivierge, K., Cwiklinski, K., Dumont, C., Tilley, L., . . . Dalton, J. P. (2021). Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (PfM1AAP) and M17 leucyl aminopeptidase (PfM17LAP).. Scientific reports, 11(1), 2854. doi:10.1038/s41598-021-82499-4

DOI
10.1038/s41598-021-82499-4
Journal article

Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver.

Cwiklinski, K., Robinson, M. W., Donnelly, S., & Dalton, J. P. (2021). Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver.. BMC genomics, 22(1), 46. doi:10.1186/s12864-020-07326-y

DOI
10.1186/s12864-020-07326-y
Journal article

2020

Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed ‘clamp-like’ mechanism of binding and inhibition

Pritsch, I. C., Tikhonova, I. G., Jewhurst, H. L., Drysdale, O., Cwiklinski, K., Molento, M. B., . . . Verissimo, C. D. M. (2020). Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed 'clamp-like' mechanism of binding and inhibition.. BMC molecular and cell biology, 21(1), 90. doi:10.1186/s12860-020-00335-5

DOI
10.1186/s12860-020-00335-5
Journal article

Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity

Murphy, A., Cwiklinski, K., Lalor, R., O'Connell, B., Robinson, M. W., Gerlach, J., . . . O'Neill, S. M. (2020). Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.. PLoS neglected tropical diseases, 14(9), e0008626. doi:10.1371/journal.pntd.0008626

DOI
10.1371/journal.pntd.0008626
Journal article

Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs).

Shiels, J., Cwiklinski, K., Alvarado, R., Thivierge, K., Cotton, S., Gonzales Santana, B., . . . Dalton, J. P. (2020). Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs).. PLoS neglected tropical diseases, 14(7), e0008470. doi:10.1371/journal.pntd.0008470

DOI
10.1371/journal.pntd.0008470
Journal article

2019

Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts.

Zhang, X. -X., Cwiklinski, K., Hu, R. -S., Zheng, W. -B., Sheng, Z. -A., Zhang, F. -K., . . . Zhu, X. -Q. (2019). Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts.. BMC genomics, 20(1), 729. doi:10.1186/s12864-019-6103-5

DOI
10.1186/s12864-019-6103-5
Journal article

Surface molecules of extracellular vesicles secreted by the helminth pathogen Fasciola hepatica direct their internalisation by host cells

de la Torre-Escudero, E., Gerlach, J. Q., Bennett, A. P. S., Cwiklinski, K., Jewhurst, H. L., Huson, K. M., . . . Robinson, M. W. (2019). Surface molecules of extracellular vesicles secreted by the helminth pathogen Fasciola hepatica direct their internalisation by host cells.. PLoS neglected tropical diseases, 13(1), e0007087. doi:10.1371/journal.pntd.0007087

DOI
10.1371/journal.pntd.0007087
Journal article

2018

In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.

McVeigh, P., Cwiklinski, K., Garcia-Campos, A., Mulcahy, G., O'Neill, S. M., Maule, A. G., & Dalton, J. P. (2018). In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.. Scientific reports, 8(1), 11700. doi:10.1038/s41598-018-29673-3

DOI
10.1038/s41598-018-29673-3
Journal article

Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones

Hodgkinson, J. E., Cwiklinski, K., Beesley, N., Hartley, C., Allen, K., & Williams, D. (2018). Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones. Parasites and Vectors, 11, 9 pages. doi:10.1186/s13071-018-2952-z

DOI
10.1186/s13071-018-2952-z
Journal article

Infection by the Helminth Parasite Fasciola hepatica Requires Rapid Regulation of Metabolic, Virulence, and Invasive Factors to Adjust to Its Mammalian Host

Cwiklinski, K., Jewhurst, H., McVeigh, P., Barbour, T., Maule, A. G., Tort, J., . . . Dalton, J. P. (2018). Infection by the Helminth Parasite <i>Fasciola hepatica</i> Requires Rapid Regulation of Metabolic, Virulence, and Invasive Factors to Adjust to Its Mammalian Host.. Molecular & cellular proteomics : MCP, 17(4), 792-809. doi:10.1074/mcp.ra117.000445

DOI
10.1074/mcp.ra117.000445
Journal article

2017

2016

Unexpected Activity of a Novel Kunitz-type Inhibitor

Smith, D., Tikhonova, I. G., Jewhurst, H. L., Drysdale, O. C., Dvořák, J., Robinson, M. W., . . . Dalton, J. P. (2016). Unexpected Activity of a Novel Kunitz-type Inhibitor: INHIBITION OF CYSTEINE PROTEASES BUT NOT SERINE PROTEASES.. The Journal of biological chemistry, 291(37), 19220-19234. doi:10.1074/jbc.m116.724344

DOI
10.1074/jbc.m116.724344
Journal article

Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans

Garcia-Campos, A., Ravidà, A., Nguyen, D. L., Cwiklinski, K., Dalton, J. P., Hokke, C. H., . . . Mulcahy, G. (2016). Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans.. PLoS neglected tropical diseases, 10(5), e0004688. doi:10.1371/journal.pntd.0004688

DOI
10.1371/journal.pntd.0004688
Journal article

2015

The Extracellular Vesicles of the Helminth Pathogen, <i>Fasciola hepatica</i>: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis

Cwiklinski, K., de la Torre-Escudero, E., Trelis, M., Bernal, D., Dufresne, P. J., Brennan, G. P., . . . Robinson, M. W. (2015). The Extracellular Vesicles of the Helminth Pathogen, <i>Fasciola hepatica</i>: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis. MOLECULAR & CELLULAR PROTEOMICS, 14(12), 3258-3273. doi:10.1074/mcp.M115.053934

DOI
10.1074/mcp.M115.053934
Journal article

Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach

Cwiklinski, K., Allen, K., LaCourse, J., Williams, D., Paterson, S., & Hodgkinson, J. (2015). Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach. Infection, Genetics and Evolution, 32, 298-304. doi:10.1016/j.meegid.2015.03.014

DOI
10.1016/j.meegid.2015.03.014
Journal article

The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution

Cwiklinski, K., Dalton, J. P., Dufresne, P. J., La Course, J., Williams, D., Hodgkinson, J., & Paterson, S. (2015). The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution. Genome Biology, 16. doi:10.1186/s13059-015-0632-2

DOI
10.1186/s13059-015-0632-2
Journal article

Fasciola hepatica vaccine: we may not be there yet but we're on the right road.

Molina-Hernández, V., Molina-Hernández, V., Mulcahy, G., Pérez, J., Martínez-Moreno, Á., Donnelly, S., . . . Cwiklinski, K. (2015). Fasciola hepatica vaccine: we may not be there yet but we're on the right road.. Veterinary parasitology, 208(1-2), 101-111. doi:10.1016/j.vetpar.2015.01.004

DOI
10.1016/j.vetpar.2015.01.004
Journal article

2013

Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant <i>Fasciola hepatica</i>

Hodgkinson, J., Cwiklinski, K., Beesley, N. J., Paterson, S., & Williams, D. J. L. (2013). Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant <i>Fasciola hepatica</i>. PARASITOLOGY, 140(12), 1523-1533. doi:10.1017/S0031182013000528

DOI
10.1017/S0031182013000528
Journal article

Transcriptome analysis of a parasitic clade V nematode: Comparative analysis of potential molecular anthelmintic targets in Cylicostephanus goldi

Cwiklinski, K., Merga, J. Y., Lake, S. L., Hartley, C., Matthews, J. B., Paterson, S., & Hodgkinson, J. E. (2013). Transcriptome analysis of a parasitic clade V nematode: Comparative analysis of potential molecular anthelmintic targets in Cylicostephanus goldi. International Journal for Parasitology, 43(11), 917-927. doi:10.1016/j.ijpara.2013.06.010

DOI
10.1016/j.ijpara.2013.06.010
Journal article

2012

New insights into sequence variation in the IGS region of 21 cyathostomin species and the implication for molecular identification.

Cwiklinski, K., Kooyman, F. N. J., Van Doorn, D. C. K., Matthews, J. B., & Hodgkinson, J. E. (2012). New insights into sequence variation in the IGS region of 21 cyathostomin species and the implication for molecular identification.. Parasitology, 139(8), 1063-1073. doi:10.1017/s0031182012000467

DOI
10.1017/s0031182012000467
Journal article

2009

Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene.

Cwiklinski, K., Meskill, D., Robinson, M. W., Pozio, E., Appleton, J. A., & Connolly, B. (2009). Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene.. Veterinary parasitology, 159(3-4), 268-271. doi:10.1016/j.vetpar.2008.10.036

DOI
10.1016/j.vetpar.2008.10.036
Journal article