Publications
Selected publications
- Functional analysis of epilepsy‐associated variants in STXBP1/Munc18‐1 using humanized Caenorhabditis elegans (Journal article - 2020)
- Ethanol Stimulates Locomotion via a G(alpha s)-Signaling Pathway in IL2 Neurons in Caenorhabditis elegans (Journal article - 2017)
- Small Heat Shock Proteins Are Novel Common Determinants of Alcohol and Nicotine Sensitivity in Caenorhabditis elegans (Journal article - 2016)
- Functional validity, role, and implications of heavy alcohol consumption genetic loci (Journal article - 2020)
- Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension (Journal article - 2021)
2024
Functional analysis of epilepsy-associated GABA<sub>A</sub> receptor mutations using Caenorhabditis elegans.
Gadhia, A., Barker, E., Morgan, A., & Barclay, J. W. (2024). Functional analysis of epilepsy-associated GABA<sub>A</sub> receptor mutations using Caenorhabditis elegans.. Epilepsia open, 9(4), 1458-1466. doi:10.1002/epi4.12982
Tissue distribution of cysteine string protein/DNAJC5 in C. elegans analysed by CRISPR/Cas9-mediated tagging of endogenous DNJ-14.
Barker, E., Morgan, A., & Barclay, J. W. (2024). Tissue distribution of cysteine string protein/DNAJC5 in C. elegans analysed by CRISPR/Cas9-mediated tagging of endogenous DNJ-14.. Cell and tissue research, 396(1), 41-55. doi:10.1007/s00441-024-03875-w
Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome.
Barker, E., Milburn, A., Helassa, N., Hammond, D., Sanchez-Soriano, N., Morgan, A., & Barclay, J. (2024). Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome.. The Biochemical journal, BCJ20230319. doi:10.1042/bcj20230319
2023
Evidence for involvement of the alcohol consumption WDPCP gene in lipid metabolism, and liver cirrhosis.
O'Farrell, F., Aleyakpo, B., Mustafa, R., Jiang, X., Pinto, R. C., Elliott, P., . . . Pazoki, R. (2023). Evidence for involvement of the alcohol consumption WDPCP gene in lipid metabolism, and liver cirrhosis.. Scientific reports, 13(1), 20616. doi:10.1038/s41598-023-47371-7
A Caenorhabditis elegans model of autosomal dominant adult-onset neuronal ceroid lipofuscinosis identifies ethosuximide as a potential therapeutic
Barker, E., Morgan, A., & Barclay, J. W. (2023). A Caenorhabditis elegans model of autosomal dominant adult-onset neuronal ceroid lipofuscinosis identifies ethosuximide as a potential therapeutic. HUMAN MOLECULAR GENETICS, 32(11), 1772-1785. doi:10.1093/hmg/ddac263
Evidence for Involvement of the Alcohol Consumption WDPCP Gene in Lipid Metabolism, and Liver Cirrhosis
Evidence for Involvement of<i>WDPCP</i>Gene in Alcohol Consumption, Lipid Metabolism, and Liver Cirrhosis
<i>C. elegans dkf-1</i> (Protein Kinase D1) mutants have age-dependent defects in locomotion and neuromuscular transmission.
Johnson, J. R., & Barclay, J. W. (2023). <i>C. elegans dkf-1</i> (Protein Kinase D1) mutants have age-dependent defects in locomotion and neuromuscular transmission.. microPublication biology, 2023. doi:10.17912/micropub.biology.000800
2022
Data-driven learning of narcosis mode of action identifies a CNS transcriptional signature shared between whole organism Caenorhabditis elegans and a fish gill cell line
Brockmeier, E. K., Basili, D., Herbert, J., Rendal, C., Boakes, L., Grauslys, A., . . . Falciani, F. (2022). Data-driven learning of narcosis mode of action identifies a CNS transcriptional signature shared between whole organism Caenorhabditis elegans and a fish gill cell line. SCIENCE OF THE TOTAL ENVIRONMENT, 849. doi:10.1016/j.scitotenv.2022.157666
2021
Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension
Campion, R., Bloxam, L., Burrow, K., Brownridge, P. J., Pentland, D. R., Thomas, P., . . . Morgan, A. (2021). Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension. BIOCHEMICAL JOURNAL, 478(24), 4153-4167. doi:10.1042/BCJ20210432
2020
Functional analysis of epilepsy‐associated variants in STXBP1/Munc18‐1 using humanized Caenorhabditis elegans
Morgan, A. (2020). Functional analysis of epilepsy‐associated variants in STXBP1/Munc18‐1 using humanized Caenorhabditis elegans. Epilepsia. doi:10.1111/epi.16464
Functional validity, role, and implications of heavy alcohol consumption genetic loci
Thompson, A., Cook, J., Choquet, H., Jorgenson, E., Yin, J., Kinnunen, T., . . . Pirmohamed, M. (2020). Functional validity, role, and implications of heavy alcohol consumption genetic loci. Science Advances, 6(3), eaay5034. doi:10.1126/sciadv.aay5034
2019
IDENTIFYING AND CONFIRMING THE FUNCTIONAL VALIDITY OF GENETIC LOCI IN ALCOHOL CONSUMPTION USING UK BIOBANK
Thompson, A., Cook, J. P., Kinnunen, T., Barclay, J., Morris, A. P., & Pirmohamed, M. (2019). IDENTIFYING AND CONFIRMING THE FUNCTIONAL VALIDITY OF GENETIC LOCI IN ALCOHOL CONSUMPTION USING UK BIOBANK. In ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH Vol. 43 (pp. 203A). Retrieved from https://www.webofscience.com/
2018
A <i>Caenorhabditis elegans</i> assay of seizure-like activity optimised for identifying antiepileptic drugs and their mechanisms of action
Wong, S. Q., Jones, A., Dodd, S., Grimes, D., Barclay, J. W., Marson, A. G., . . . Morgan, A. (2018). A <i>Caenorhabditis elegans</i> assay of seizure-like activity optimised for identifying antiepileptic drugs and their mechanisms of action. JOURNAL OF NEUROSCIENCE METHODS, 309, 132-142. doi:10.1016/j.jneumeth.2018.09.004
α-Methyl-α-phenylsuccinimide ameliorates neurodegeneration in a C. elegans model of TDP-43 proteinopathy
Wong, S. Q., Pontifex, M. G., Phelan, M. M., Pidathala, C., Kraemer, B. C., Barclay, J. W., . . . Morgan, A. (2018). α-Methyl-α-phenylsuccinimide ameliorates neurodegeneration in a C. elegans model of TDP-43 proteinopathy. Neurobiology of disease, 118, 40-54. doi:10.1016/j.nbd.2018.06.013
2017
Ethanol Stimulates Locomotion via a G(alpha s)-Signaling Pathway in IL2 Neurons in Caenorhabditis elegans
Johnson, J. R., Edwards, M. R., Davies, H., Newman, D., Holden, W., Jenkins, R. E., . . . Barclay, J. W. (2017). Ethanol Stimulates Locomotion via a G(alpha s)-Signaling Pathway in IL2 Neurons in Caenorhabditis elegans. GENETICS, 207(3), 1023-1039. doi:10.1534/genetics.117.300119
2016
Interaction of ARF-1.1 and neuronal calcium sensor-1 in the control of the temperature-dependency of locomotion in Caenorhabditis elegans
Todd, P. A. C., McCue, H. V., Haynes, L. P., Barclay, J. W., & Burgoyne, R. D. (2016). Interaction of ARF-1.1 and neuronal calcium sensor-1 in the control of the temperature-dependency of locomotion in Caenorhabditis elegans. Scientific Reports, 6. doi:10.1038/srep30023
Small Heat Shock Proteins Are Novel Common Determinants of Alcohol and Nicotine Sensitivity in Caenorhabditis elegans
Johnson, J. R., Rajamanoharan, D., McCue, H. V., Rankin, K., & Barclay, J. W. (2016). Small Heat Shock Proteins Are Novel Common Determinants of Alcohol and Nicotine Sensitivity in Caenorhabditis elegans. Genetics, 202(3), 1013-1027. doi:10.1534/genetics.115.185025
2015
Using <i>C</i>. <i>elegans</i> to discover therapeutic compounds for ageing-associated neurodegenerative diseases
Chen, X., Barclay, J. W., Burgoyne, R. D., & Morgan, A. (2015). Using <i>C</i>. <i>elegans</i> to discover therapeutic compounds for ageing-associated neurodegenerative diseases. CHEMISTRY CENTRAL JOURNAL, 9. doi:10.1186/s13065-015-0143-y
Ethosuximide ameliorates neurodegenerative disease phenotypes by modulating DAF-16/FOXO target gene expression (vol 10, 51, 2015)
Chen, X., McCue, H. V., Wong, S. Q., Kashyap, S. S., Kraemer, B. C., Barclay, J. W., . . . Morgan, A. (2015). Ethosuximide ameliorates neurodegenerative disease phenotypes by modulating DAF-16/FOXO target gene expression (vol 10, 51, 2015). MOLECULAR NEURODEGENERATION, 10. doi:10.1186/s13024-015-0051-6
Ethosuximide ameliorates neurodegenerative disease phenotypes by modulating DAF-16/FOXO target gene expression
Chen, X., McCuef, H. V., Wong, S. Q., Kashyap, S. S., Kraemer, B. C., Barclay, J. W., . . . Morgan, A. (2015). Ethosuximide ameliorates neurodegenerative disease phenotypes by modulating DAF-16/FOXO target gene expression. Molecular Neurodegeneration, 10. doi:10.1186/s13024-015-0046-3
Expression profile of a Caenorhabditis elegans model of adult neuronal ceroid lipofuscinosis reveals down regulation of ubiquitin E3 ligase components.
McCue, H., Chen, X., Barclay, J., Morgan, A., & Burgoyne, R. D. (2015). Expression profile of a Caenorhabditis elegans model of adult neuronal ceroid lipofuscinosis reveals down regulation of ubiquitin E3 ligase components.. Scientific reports, 5. doi:10.1038/srep14392
2014
Caenorhabditis elegans dnj-14, the orthologue of the DNAJC5 gene mutated in adult onset neuronal ceroid lipofuscinosis, provides a new platform for neuroprotective drug screening and identifies a SIR-2.1-independent action of resveratrol
Kashyap, S. S., Johnson, J. R., McCue, H., Chen, X., Edmonds, M. J., Ayala, M., . . . Morgan, A. (2014). Caenorhabditis elegans dnj-14, the orthologue of the DNAJC5 gene mutated in adult onset neuronal ceroid lipofuscinosis, provides a new platform for neuroprotective drug screening and identifies a SIR-2.1-independent action of resveratrol. Human Molecular Genetics, 23(22), 5916-5927. doi:10.1093/hmg/ddu316
2013
<i>Rab-3</i> and <i>unc-18</i> Interactions in Alcohol Sensitivity Are Distinct from Synaptic Transmission
Johnson, J. R., Kashyap, S., Rankin, K., & Barclay, J. W. (2013). <i>Rab-3</i> and <i>unc-18</i> Interactions in Alcohol Sensitivity Are Distinct from Synaptic Transmission. PLOS ONE, 8(11). doi:10.1371/journal.pone.0081117
Using Caenorhabditis elegans to fight human neurodegenerative diseases
Chen, X., Kraemer, B., Barclay, J. W., Burgoyne, R., & Morgan, A. (2013). Using Caenorhabditis elegans to fight human neurodegenerative diseases. Molecular Neurodegeneration. doi:10.1186/1750-1326-8-S1-P12
Identification of key structural elements for neuronal calcium sensor-1 function in the regulation of the temperature-dependency of locomotion in C. elegans
Martin, V. M., Johnson, J. R., Haynes, L. P., Barclay, J. W., & Burgoyne, R. D. (2013). Identification of key structural elements for neuronal calcium sensor-1 function in the regulation of the temperature-dependency of locomotion in C. elegans. MOLECULAR BRAIN, 6. doi:10.1186/1756-6606-6-39
Using Caenorhabditis elegans to fight human neurodegenerative diseases
Chen, X., Kraemer, B., Barclay, J., Burgoyne, R., & Morgan, A. (2013). Using Caenorhabditis elegans to fight human neurodegenerative diseases.
2012
PKC-2 Phosphorylation of UNC-18 Ser322 in AFD Neurons Regulates Temperature Dependency of Locomotion
Edwards, M. R., Johnson, J. R., Rankin, K., Jenkins, R. E., Maguire, C., Morgan, A., . . . Barclay, J. W. (2012). PKC-2 Phosphorylation of UNC-18 Ser322 in AFD Neurons Regulates Temperature Dependency of Locomotion. JOURNAL OF NEUROSCIENCE, 32(20), 7042-7051. doi:10.1523/JNEUROSCI.4029-11.2012
Neurotransmitter release mechanisms studied in <i>Caenorhabditis elegans</i>
Barclay, J. W., Morgan, A., & Burgoyne, R. D. (2012). Neurotransmitter release mechanisms studied in <i>Caenorhabditis elegans</i>. CELL CALCIUM, 52(3-4), 289-295. doi:10.1016/j.ceca.2012.03.005
2011
Munc18-1 Tuning of Vesicle Merger and Fusion Pore Properties
Jorgacevski, J., Potokar, M., Grilc, S., Kreft, M., Liu, W., Barclay, J. W., . . . Zorec, R. (2011). Munc18-1 Tuning of Vesicle Merger and Fusion Pore Properties. JOURNAL OF NEUROSCIENCE, 31(24), 9055-9066. doi:10.1523/JNEUROSCI.0185-11.2011
Structure-Function Study of Mammalian Munc18-1 and <i>C</i>. <i>elegans</i> UNC-18 Implicates Domain 3b in the Regulation of Exocytosis
Graham, M. E., Prescott, G. R., Johnson, J. R., Jones, M., Walmesley, A., Haynes, L. P., . . . Barclay, J. W. (2011). Structure-Function Study of Mammalian Munc18-1 and <i>C</i>. <i>elegans</i> UNC-18 Implicates Domain 3b in the Regulation of Exocytosis. PLOS ONE, 6(3). doi:10.1371/journal.pone.0017999
2010
<i>Caenorhabditis elegans</i>: a useful tool to decipher neurodegenerative pathways
Johnson, J. R., Jenn, R. C., Barclay, J. W., Burgoyne, R. D., & Morgan, A. (2010). <i>Caenorhabditis elegans</i>: a useful tool to decipher neurodegenerative pathways. BIOCHEMICAL SOCIETY TRANSACTIONS, 38, 559-563. doi:10.1042/BST0380559
Presynaptic targets for acute ethanol sensitivity
Barclay, J. W., Graham, M. E., Edwards, M. R., Johnson, J. R., Morgan, A., & Burgoyne, R. D. (2010). Presynaptic targets for acute ethanol sensitivity. BIOCHEMICAL SOCIETY TRANSACTIONS, 38, 172-176. doi:10.1042/BST0380172
2009
Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in <i>Caenorhabditis elegans</i>
Johnson, J. R., Ferdek, P., Lian, L. -Y., Barclay, J. W., Burgoyne, R. D., & Morgan, A. (2009). Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in <i>Caenorhabditis elegans</i>. BIOCHEMICAL JOURNAL, 418, 73-80. doi:10.1042/BJ20081956
The Functions of Munc18-1 in Regulated Exocytosis
Burgoyne, R. D., Barclay, J. W., Ciufo, L. E., Graham, M. E., Handley, M. T. W., & Morgan, A. (2009). The Functions of Munc18-1 in Regulated Exocytosis. MECHANISMS OF EXOCYTOSIS, 1152, 76-86. doi:10.1111/j.1749-6632.2008.03987.x
UNC-18 Modulates Ethanol Sensitivity in <i>Caenorhabditis elegans</i>
Graham, M. E., Edwards, M. R., Holden-Dye, L., Morgan, A., Burgoyne, R. D., & Barclay, J. W. (2009). UNC-18 Modulates Ethanol Sensitivity in <i>Caenorhabditis elegans</i>. MOLECULAR BIOLOGY OF THE CELL, 20(1), 43-55. doi:10.1091/mbc.E08-07-0689
2008
A random mutagenesis approach to isolate dominant-negative yeast Sec1 mutants reveals a functional role for domain 3a in yeast and mammalian Sec1/Munc18 proteins
Boyd, A., Ciufo, L. F., Barclay, J. W., Graham, M. E., Haynes, L. P., Doherty, M. K., . . . Morgan, A. (2008). A random mutagenesis approach to isolate dominant-negative yeast Sec1 mutants reveals a functional role for domain 3a in yeast and mammalian Sec1/Munc18 proteins. GENETICS, 180(1), 165-178. doi:10.1534/genetics.108.090423
S-nitrosylation of syntaxin 1 at Cys<SUP>145</SUP> is a regulatory switch controlling Munc18-1 binding
Palmer, Z. J., Duncan, R. R., Johnson, J. R., Lian, L. -Y., Mello, L. V., Booth, D., . . . Morgan, A. (2008). S-nitrosylation of syntaxin 1 at Cys<SUP>145</SUP> is a regulatory switch controlling Munc18-1 binding. BIOCHEMICAL JOURNAL, 413, 479-491. doi:10.1042/BJ20080069
Munc-18-1 regulates the initial release rate of exocytosis.
Barclay, J. W. (2008). Munc-18-1 regulates the initial release rate of exocytosis.. Biophysical journal, 94(3), 1084-1093. doi:10.1529/biophysj.107.111203
A gain-of-function mutant of Munc18-1 stimulates secretory granule recruitment and exocytosis and reveals a direct interaction of Munc18-1 with Rab3
Graham, M. E., Handley, M. T. W., Barclay, J. W., Ciufo, L. F., Barrow, S. L., Morgan, A., & Burgoyne, R. D. (2008). A gain-of-function mutant of Munc18-1 stimulates secretory granule recruitment and exocytosis and reveals a direct interaction of Munc18-1 with Rab3. BIOCHEMICAL JOURNAL, 409, 407-416. doi:10.1042/BJ20071094
2006
Protein kinase B/Akt is a novel cysteine string protein kinase that regulates exocytosis release kinetics and quantal size
Evans, G. J. O., Barclay, J. W., Prescott, G. R., Jo, S. R., Burgoyne, R. D., Birnbaum, M. J., & Morgan, A. (2006). Protein kinase B/Akt is a novel cysteine string protein kinase that regulates exocytosis release kinetics and quantal size. JOURNAL OF BIOLOGICAL CHEMISTRY, 281(3), 1564-1572. doi:10.1074/jbc.M503628200
2005
Regulation of exocytosis by protein kinase C.
Morgan, A., Burgoyne, R. D., Barclay, J. W., Craig, T. J., Prescott, G. R., Ciufo, L. F., . . . Graham, M. E. (2005). Regulation of exocytosis by protein kinase C.. In Biochemical Society transactions Vol. 33 (pp. 1341-1344). doi:10.1042/bst0331341
Regulation of exocytosis by protein kinase C
Morgan, A., Burgoyne, R. D., Barclay, J. W., Craig, T. J., Prescott, G. R., Ciufo, L. F., . . . Graham, M. E. (2005). Regulation of exocytosis by protein kinase C. BIOCHEMICAL SOCIETY TRANSACTIONS, 33, 1341-1344. doi:10.1042/BST0331341
Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions
Ciufo, L. F., Barclay, J. W., Burgoyne, R. D., & Morgan, A. (2005). Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. MOLECULAR BIOLOGY OF THE CELL, 16(2), 470-482. doi:10.1091/mbc.E04-08-0685
Calcium-dependent regulation of exocytosis
Barclay, J. W., Morgan, A., & Burgoyne, R. D. (2005). Calcium-dependent regulation of exocytosis. CELL CALCIUM, 38(3-4), 343-353. doi:10.1016/j.ceca.2005.06.012
2004
Regulation of the fusion pore conductance during exocytosis by cyclin-dependent kinase 5
Barclay, J. W., Aldea, M., Craig, T. J., Morgan, A., & Burgoyne, R. D. (2004). Regulation of the fusion pore conductance during exocytosis by cyclin-dependent kinase 5. JOURNAL OF BIOLOGICAL CHEMISTRY, 279(40), 41495-41503. doi:10.1074/jbc.M406670200
Syntaxin/Munc18 interactions in the late events during vesicle fusion and release in exocytosis
Graham, M. E., Barclay, J. W., & Burgoyne, R. D. (2004). Syntaxin/Munc18 interactions in the late events during vesicle fusion and release in exocytosis. JOURNAL OF BIOLOGICAL CHEMISTRY, 279(31), 32751-32760. doi:10.1074/jbc.M400827200
2003
Role for calcium in heat shock-mediated synaptic thermoprotection in Drosophila larvae.
Barclay, J. W., & Robertson, R. M. (2003). Role for calcium in heat shock-mediated synaptic thermoprotection in Drosophila larvae.. Journal of neurobiology, 56(4), 360-371. doi:10.1002/neu.10247
Phosphorylation of Munc18 by protein kinase C regulates the kinetics of exocytosis
Barclay, J. W., Craig, T. J., Fisher, R. J., Ciufo, L. F., Evans, G. J. O., Morgan, A., & Burgoyne, R. D. (2003). Phosphorylation of Munc18 by protein kinase C regulates the kinetics of exocytosis. JOURNAL OF BIOLOGICAL CHEMISTRY, 278(12), 10538-10545. doi:10.1074/jbc.M211114200
2002
Enhancement of presynaptic performance in transgenic Drosophila overexpressing heat shock protein Hsp70.
Karunanithi, S., Barclay, J. W., Brown, I. R., Robertson, R. M., & Atwood, H. L. (2002). Enhancement of presynaptic performance in transgenic Drosophila overexpressing heat shock protein Hsp70.. Synapse (New York, N.Y.), 44(1), 8-14. doi:10.1002/syn.10048
Splitting the quantum: regulation of quantal release during vesicle fusion
Burgoyne, R. D., & Barclay, J. W. (2002). Splitting the quantum: regulation of quantal release during vesicle fusion. TRENDS IN NEUROSCIENCES, 25(4), 176-178. doi:10.1016/S0166-2236(00)02126-3
Impairment of central pattern generation in Drosophila cysteine string protein mutants.
Barclay, J. W., Atwood, H. L., & Robertson, R. M. (2002). Impairment of central pattern generation in Drosophila cysteine string protein mutants.. Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, 188(1), 71-78. doi:10.1007/s00359-002-0279-9
2001
Enhancement of short-term synaptic plasticity by prior environmental stress.
Barclay, J. W., & Robertson, R. M. (2001). Enhancement of short-term synaptic plasticity by prior environmental stress.. Journal of neurophysiology, 85(3), 1332-1335. doi:10.1152/jn.2001.85.3.1332
2000
Heat-shock-induced thermoprotection of hindleg motor control in the locust
Barclay, J. W., & Robertson, R. M. (2000). Heat-shock-induced thermoprotection of hindleg motor control in the locust. J Exp Biol, 203(Pt 5), 941-950. Retrieved from http://www.ncbi.nlm.nih.gov/
1999
Neuroprotection at Drosophila synapses conferred by prior heat shock
Karunanithi, S., Barclay, J. W., Robertson, R. M., Brown, I. R., & Atwood, H. L. (1999). Neuroprotection at Drosophila synapses conferred by prior heat shock. J Neurosci, 19(11), 4360-4369. Retrieved from http://www.ncbi.nlm.nih.gov/