Publications
2024
SHANK3 depletion leads to ERK signalling overdose and cell death in KRAS-mutant cancers.
Lilja, J., Kaivola, J., Conway, J. R. W., Vuorio, J., Parkkola, H., Roivas, P., . . . Ivaska, J. (2024). SHANK3 depletion leads to ERK signalling overdose and cell death in KRAS-mutant cancers.. Nature communications, 15(1), 8002. doi:10.1038/s41467-024-52326-1
Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis.
Chanduri, M., Kumar, A., Weiss, D., Emuna, N., Barsukov, I., Shi, M., . . . Schwartz, M. A. (2024). Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis.. Sci Adv, 10(34), eadi6286. doi:10.1126/sciadv.adi6286
Regulation of the DLC3 tumor suppressor by a novel phosphoswitch.
Frey, Y., Lungu, C., Meyer, F., Hauth, F., Hahn, D., Kersten, C., . . . Olayioye, M. A. (2024). Regulation of the DLC3 tumor suppressor by a novel phosphoswitch.. iScience, 27(7), 110203. doi:10.1016/j.isci.2024.110203
Polysaccharide sulfotransferases: the identification of putative sequences and respective functional characterisation.
Mistry, R., Byrne, D. P., Starns, D., Barsukov, I. L., Yates, E. A., & Fernig, D. G. (2024). Polysaccharide sulfotransferases: the identification of putative sequences and respective functional characterisation.. Essays in biochemistry, EBC20230094. doi:10.1042/ebc20230094
Dock-and-lock binding of SxIP ligands is required for stable and selective EB1 interactions
The Shank/ProSAP N-Terminal (SPN) Domain of Shank3 Regulates Targeting to Postsynaptic Sites and Postsynaptic Signaling.
Woike, D., Tibbe, D., Hassani Nia, F., Martens, V., Wang, E., Barsukov, I., & Kreienkamp, H. -J. (2024). The Shank/ProSAP N-Terminal (SPN) Domain of Shank3 Regulates Targeting to Postsynaptic Sites and Postsynaptic Signaling.. Molecular neurobiology, 61(2), 693-706. doi:10.1007/s12035-023-03611-5
2023
The Shank/ProSAP N-terminal (SPN) domain of Shank3 regulates targeting to postsynaptic sites and postsynaptic signalling
Detecting 2D NMR Signals Using Mask RCNN
Alghamdi, H., Lisitsa, A., Barsukov, I., & Grosman, R. (2023). Detecting 2D NMR Signals Using Mask RCNN. In Proceedings of the 15th International Conference on Agents and Artificial Intelligence (pp. 940-947). SCITEPRESS - Science and Technology Publications. doi:10.5220/0011804700003393
Interpreting NMR Spectra by Constraint Solving
Alharbi, H. A., Barsukov, I., Grosman, R., & Lisitsa, A. (2023). Interpreting NMR Spectra by Constraint Solving. In Lecture Notes in Computer Science (pp. 465-478). Springer Nature Switzerland. doi:10.1007/978-3-031-47994-6_40
2022
Tensin3 interaction with talin drives the formation of fibronectin-associated fibrillar adhesions
Atherton, P., Konstantinou, R., Neo, S. P., Wang, E., Balloi, E., Ptushkina, M., . . . Ballestrem, C. (2022). Tensin3 interaction with talin drives the formation of fibronectin-associated fibrillar adhesions. JOURNAL OF CELL BIOLOGY, 221(10). doi:10.1083/jcb.202107022
Targeting a broad spectrum of <i>KRAS</i>-mutant cancers by hyperactivation-induced cell death
Exploration of expanded carbohydrate chemical space to access biological activity using microwave-induced acid condensation of simple sugars
London, J. A., Taylor, S. L., Barsukov, I., Cartmell, A., & Yates, E. A. (2022). Exploration of expanded carbohydrate chemical space to access biological activity using microwave-induced acid condensation of simple sugars. RSC ADVANCES, 12(18), 11075-11083. doi:10.1039/d2ra01463g
Exploration of expanded carbohydrate chemical space to access biological activity using microwave-induced acid condensation of simple sugars
Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders
Woike, D., Wang, E., Tibbe, D., Hassani Nia, F., Failla, A. V., Kibaek, M., . . . Kreienkamp, H. -J. (2022). Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders. SCIENTIFIC REPORTS, 12(1). doi:10.1038/s41598-021-04723-5
Molecular Fragments from Incomplete, Real-life NMR Data: Framework for Spectra Analysis with Constraint Solvers
Alharbi, H. A., Barsukov, I., Grosman, R., & Lisitsa, A. (2022). Molecular Fragments from Incomplete, Real-life NMR Data: Framework for Spectra Analysis with Constraint Solvers. In ICAART: PROCEEDINGS OF THE 14TH INTERNATIONAL CONFERENCE ON AGENTS AND ARTIFICIAL INTELLIGENCE - VOL 3 (pp. 834-841). doi:10.5220/0010915800003116
2021
Anion binding to a cationic europium(iii) probe enables the first real-time assay of heparan sulfotransferase activity
Wheeler, S., Breen, C., Li, Y., Hewitt, S. H., Robertson, E., Yates, E. A., . . . Butler, S. J. (2021). Anion binding to a cationic europium(iii) probe enables the first real-time assay of heparan sulfotransferase activity. ORGANIC & BIOMOLECULAR CHEMISTRY, 20(3), 596-605. doi:10.1039/d1ob02071d
Tensin3 interaction with talin drives formation of fibronectin-associated fibrillar adhesions
Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders
Synthesis and toxicity profile in 293 human embryonic kidney cells of the β D-glucuronide derivatives of <i>ortho</i>-<i>, meta</i>- and <i>para</i>-cresol
London, J. A., Wang, E. C. S., Barsukov, I. L., Yates, E. A., & Stachulski, A. V. (2021). Synthesis and toxicity profile in 293 human embryonic kidney cells of the β D-glucuronide derivatives of <i>ortho</i>-<i>, meta</i>- and <i>para</i>-cresol. CARBOHYDRATE RESEARCH, 499. doi:10.1016/j.carres.2020.108225
2020
Abstract B29: SHANK3 in oncogenic RAS signaling
Lilja, J., Jcaquement, G., Posada, I. M. D., Laasola, P., Vesilahti, E. -M., Miihkinen, M., . . . Ivaska, J. (2020). Abstract B29: SHANK3 in oncogenic RAS signaling. Molecular Cancer Research, 18(5_Supplement), B29. doi:10.1158/1557-3125.ras18-b29
Direct interaction of metastasis-inducing S100P protein with tubulin causes enhanced cell migration without changes in cell adhesion
Du, M., Wang, G., Barsukov, I. L., Gross, S. R., Smith, R., & Rudland, P. S. (2020). Direct interaction of metastasis-inducing S100P protein with tubulin causes enhanced cell migration without changes in cell adhesion. BIOCHEMICAL JOURNAL, 477(6), 1159-1178. doi:10.1042/BCJ20190644
Relief of talin autoinhibition triggers a force-independent association with vinculin
Atherton, P., Lausecker, F., Carisey, A., Gilmore, A., Critchley, D., Barsukov, I., & Ballestrem, C. (2020). Relief of talin autoinhibition triggers a force-independent association with vinculin. JOURNAL OF CELL BIOLOGY, 219(1). doi:10.1083/jcb.201903134
SHANK3 in oncogenic RAS signaling.
Lilja, J., Jcaquement, G., Posada, I. M. D., Laasola, P., Vesilahti, E. -M., Miihkinen, M., . . . Ivaska, J. (2020). SHANK3 in oncogenic RAS signaling.. In MOLECULAR CANCER RESEARCH Vol. 18 (pp. 67). Retrieved from https://www.webofscience.com/
2019
A further unique chondroitin sulfate from the shrimp <i>Litopenaeus vannamei</i> with antithrombin activity that modulates acute inflammation
Palhares, L. C. G. F., Brito, A. S., de Lima, M. A., Nader, H. B., London, J. A., Barsukov, I. L., . . . Chavante, S. F. (2019). A further unique chondroitin sulfate from the shrimp <i>Litopenaeus vannamei</i> with antithrombin activity that modulates acute inflammation. CARBOHYDRATE POLYMERS, 222. doi:10.1016/j.carbpol.2019.115031
Force-independent interactions of talin and vinculin govern integrin-mediated mechanotransduction
2018
New tools for carbohydrate sulfation analysis: heparan sulfate 2-O-sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors
Byrne, D. P., Li, Y., Ramakrishnan, K., Barsukov, I. L., Yates, E. A., Eyers, C. E., . . . Eyers, P. A. (2018). New tools for carbohydrate sulfation analysis: heparan sulfate 2-O-sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors. BIOCHEMICAL JOURNAL, 475(15), 2417-2433. doi:10.1042/BCJ20180265
Co-ordination of mechanotransduction by talin and vinculin
Atherton, P., Lausecker, F., Barsukov, I., Critchley, D., & Ballestrem, C. (2018). Co-ordination of mechanotransduction by talin and vinculin. In INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY Vol. 99 (pp. A21). Retrieved from https://www.webofscience.com/
SHANK3 in oncogenic RAS signaling
Lilja, J., Jacquemet, G., Posada, I. M. D., Laasola, P., Vesilahti, E., Miihkinen, M., . . . Ivaska, J. (2018). SHANK3 in oncogenic RAS signaling. In MOLECULAR BIOLOGY OF THE CELL Vol. 29 (pp. 223). Retrieved from https://www.webofscience.com/
2017
Pressure-Dependent Chemical Shifts in the R3 Domain of Talin Show that It Is Thermodynamically Poised for Binding to Either Vinculin or RIAM
Baxter, N. J., Zacharchenko, T., Barsukov, I. L., & Williamson, M. P. (2017). Pressure-Dependent Chemical Shifts in the R3 Domain of Talin Show that It Is Thermodynamically Poised for Binding to Either Vinculin or RIAM. Structure, 25(12), 1856-1866.e2. doi:10.1016/j.str.2017.10.008
Targeting SxIP-EB1 interaction: An integrated approach to the discovery of small molecule modulators of dynamic binding sites
Almeida, T. B., Carnell, A. J., Barsukov, I. L., & Berry, N. G. (2017). Targeting SxIP-EB1 interaction: An integrated approach to the discovery of small molecule modulators of dynamic binding sites. SCIENTIFIC REPORTS, 7. doi:10.1038/s41598-017-15502-6
SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras
Lilja, J., Zacharchenko, T., Georgiadou, M., Jacquemet, G., Franceschi, N. D., Peuhu, E., . . . Ivaska, J. (2017). SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras. Nature Cell Biology, 19(4), 292-305. doi:10.1038/ncb3487
2016
Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold
Zacharchenko, T., Barsukov, I., Rigden, D. J., Bennett, D., & Mayans, O. (2016). Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold. Protein Journal, 35(5), 240-345. doi:10.1007/s10930-016-9677-7
CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex
Zhou, T., Fleming, J. R., Franke, B., Bogomolovas, J., Barsukov, I., Rigden, D. J., . . . Mayans, O. (2016). CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex. FEBS LETTERS, 590(18), 3098-3110. doi:10.1002/1873-3468.12362
LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex
Zacharchenko, T., Qian, X., Goult, B. T., Jethwa, D., Almeida, T. B., Ballestrem, C., . . . Barsukov, I. L. (2016). LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex. Structure, 24(7), 1130-1141. doi:10.1016/j.str.2016.04.016
SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation.
Lilja, J., Zacharchenko, T., Georgiadou, M., Kreienkamp, H., Barsukov, I., & Ivaska, J. (2016). SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation.. In MOLECULAR BIOLOGY OF THE CELL Vol. 27. Retrieved from https://www.webofscience.com/
SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation.
Lilja, J., Zacharchenko, T., Georgiadou, M., Kreienkamp, H., Barsukov, I., & Ivaska, J. (2016). SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation.. In MOLECULAR BIOLOGY OF THE CELL Vol. 27. Retrieved from https://www.webofscience.com/
2015
Vinculin controls talin engagement with the actomyosin machinery.
Atherton, P., Stutchbury, B., Wang, D. -Y., Jethwa, D., Tsang, R., Meiler-Rodriguez, E., . . . Ballestrem, C. (2015). Vinculin controls talin engagement with the actomyosin machinery.. Nature communications, 6. doi:10.1038/ncomms10038
Parallel evolution or purifying selection, not introgression, explains similarity in the pyrethroid detoxification linked GSTE4 of <i>Anopheles gambiae</i> and <i>An. arabiensis</i>
Wilding, C. S., Weetman, D., Rippon, E. J., Steen, K., Mawejje, H. D., Barsukov, I., & Donnelly, M. J. (2015). Parallel evolution or purifying selection, not introgression, explains similarity in the pyrethroid detoxification linked GSTE4 of <i>Anopheles gambiae</i> and <i>An. arabiensis</i>. MOLECULAR GENETICS AND GENOMICS, 290(1), 201-215. doi:10.1007/s00438-014-0910-9
2014
S100A4 regulates the Src-tyrosine kinase dependent differentiation of Th17 cells in rheumatoid arthritis
Brisslert, M., Bian, L., Svensson, M. N. D., Santos, R. F., Jonsson, I. -M., Barsukov, I., . . . Bokarewa, M. I. (2014). S100A4 regulates the Src-tyrosine kinase dependent differentiation of Th17 cells in rheumatoid arthritis. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1842(11), 2049-2059. doi:10.1016/j.bbadis.2014.07.003
S100A4 REGULATES THE SRC-THYROSINE KINASE DEPENDENT DIFFERENTIATION OF TH17 CELLS IN RHEUMATOID ARTHRITIS
Brisslert, M., Bian, L., Svensson, M., Santos, R., Jonsson, I. -M., Erlandsson, M., . . . Bokarewa, M. (2014). S100A4 REGULATES THE SRC-THYROSINE KINASE DEPENDENT DIFFERENTIATION OF TH17 CELLS IN RHEUMATOID ARTHRITIS. In ANNALS OF THE RHEUMATIC DISEASES Vol. 73 (pp. 129). doi:10.1136/annrheumdis-2014-eular.5414
2013
Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: implications for talin activation.
Goult, B. T., Xu, X. -P., Gingras, A. R., Swift, M., Patel, B., Bate, N., . . . Hanein, D. (2013). Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: implications for talin activation.. Journal of structural biology, 184(1), 21-32. doi:10.1016/j.jsb.2013.05.014
<i>SHANK3</i> Gene Mutations Associated with Autism Facilitate Ligand Binding to the Shank3 Ankyrin Repeat Region
Mameza, M. G., Dvoretskova, E., Bamann, M., Hoenck, H. -H., Gueler, T., Boeckers, T. M., . . . Kreienkamp, H. -J. (2013). <i>SHANK3</i> Gene Mutations Associated with Autism Facilitate Ligand Binding to the Shank3 Ankyrin Repeat Region. JOURNAL OF BIOLOGICAL CHEMISTRY, 288(37), 26697-26708. doi:10.1074/jbc.M112.424747
DEUBIQUITYLASES FROM GENES TO ORGANISM
Clague, M. J., Barsukov, I., Coulson, J. M., Liu, H., Rigden, D. J., & Urbe, S. (2013). DEUBIQUITYLASES FROM GENES TO ORGANISM. PHYSIOLOGICAL REVIEWS, 93(3), 1289-1315. doi:10.1152/physrev.00002.2013
Deubiquitylases From Genes to Organism
Clague, M. J., Barsukov, I., Coulson, J. M., Liu, H., Rigden, D. J., & Urbé, S. (2013). Deubiquitylases From Genes to Organism. Physiological Reviews, 93(3), 1289-1315. doi:10.1152/physrev.00002.2013
RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover.
Goult, B. T., Zacharchenko, T., Bate, N., Tsang, R., Hey, F., Gingras, A. R., . . . Barsukov, I. L. (2013). RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover.. The Journal of biological chemistry, 288(12), 8238-8249. doi:10.1074/jbc.m112.438119
NMR Methods for Kinetic Analysis
Barsukov, I. L. (2013). NMR Methods for Kinetic Analysis. In Encyclopedia of Biophysics (pp. 1724-1727). Springer Berlin Heidelberg. doi:10.1007/978-3-642-16712-6_65
2012
The intracellular Ig fold: a robust protein scaffold for the engineering of molecular recognition.
Bruning, M., Barsukov, I., Franke, B., Barbieri, S., Volk, M., Leopoldseder, S., . . . Mayans, O. (2012). The intracellular Ig fold: a robust protein scaffold for the engineering of molecular recognition.. Protein engineering, design & selection : PEDS, 25(5), 205-212. doi:10.1093/protein/gzs007
Asymmetric Mode of Ca<SUP>2+</SUP>-S100A4 Interaction with Nonmuscle Myosin IIA Generates Nanomolar Affinity Required for Filament Remodeling
Elliott, P. R., Irvine, A. F., Jung, H. S., Tozawa, K., Pastok, M. W., Picone, R., . . . Barsukov, I. L. (2012). Asymmetric Mode of Ca<SUP>2+</SUP>-S100A4 Interaction with Nonmuscle Myosin IIA Generates Nanomolar Affinity Required for Filament Remodeling. STRUCTURE, 20(4), 654-666. doi:10.1016/j.str.2012.02.002
A conserved lipid-binding loop in the kindlin FERM F1 domain is required for kindlin-mediated αIIbβ3 integrin coactivation.
Bouaouina, M., Goult, B. T., Huet-Calderwood, C., Bate, N., Brahme, N. N., Barsukov, I. L., . . . Calderwood, D. A. (2012). A conserved lipid-binding loop in the kindlin FERM F1 domain is required for kindlin-mediated αIIbβ3 integrin coactivation.. The Journal of biological chemistry, 287(10), 6979-6990. doi:10.1074/jbc.m111.330845
2011
Structural variability of the ubiquitin specific protease DUSP-UBL double domains
Elliott, P. R., Liu, H., Pastok, M. W., Grossmann, G. J., Rigden, D. J., Clague, M. J., . . . Barsukov, I. L. (2011). Structural variability of the ubiquitin specific protease DUSP-UBL double domains. FEBS LETTERS, 585(21), 3385-3390. doi:10.1016/j.febslet.2011.09.040
Resonance Assignments
Lian, L., & Barsukov, I. L. (2011). Resonance Assignments. In Unknown Book (pp. 55-82). Wiley. doi:10.1002/9781119972006.ch3
A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain
Hughes, E., Whittaker, C. A. P., Barsukov, I. L., Esmann, M., & Middleton, D. A. (2011). A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1808(4), 1021-1031. doi:10.1016/j.bbamem.2010.11.024
A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain.
Hughes, E., Whittaker, C. A. P., Barsukov, I. L., Esmann, M., & Middleton, D. A. (2011). A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain.. Biochimica et biophysica acta, 1808(4), 1021-1031. doi:10.1016/j.bbamem.2010.11.024
Mechanism of the Ca²+-dependent interaction between S100A4 and tail fragments of nonmuscle myosin heavy chain IIA.
Badyal, S. K., Basran, J., Bhanji, N., Kim, J. H., Chavda, A. P., Jung, H. S., . . . Bagshaw, C. R. (2011). Mechanism of the Ca²+-dependent interaction between S100A4 and tail fragments of nonmuscle myosin heavy chain IIA.. Journal of molecular biology, 405(4), 1004-1026. doi:10.1016/j.jmb.2010.11.036
2010
The Structure of the talin head reveals a novel extended conformation of the FERM domain.
Elliott, P. R., Goult, B. T., Kopp, P. M., Bate, N., Grossmann, J. G., Roberts, G. C. K., . . . Barsukov, I. L. (2010). The Structure of the talin head reveals a novel extended conformation of the FERM domain.. Structure (London, England : 1993), 18(10), 1289-1299. doi:10.1016/j.str.2010.07.011
Central region of talin has a unique fold that binds vinculin and actin.
Gingras, A. R., Bate, N., Goult, B. T., Patel, B., Kopp, P. M., Emsley, J., . . . Critchley, D. R. (2010). Central region of talin has a unique fold that binds vinculin and actin.. The Journal of biological chemistry, 285(38), 29577-29587. doi:10.1074/jbc.m109.095455
The domain structure of talin: residues 1815-1973 form a five-helix bundle containing a cryptic vinculin-binding site.
Goult, B. T., Gingras, A. R., Bate, N., Barsukov, I. L., Critchley, D. R., & Roberts, G. C. K. (2010). The domain structure of talin: residues 1815-1973 form a five-helix bundle containing a cryptic vinculin-binding site.. FEBS letters, 584(11), 2237-2241. doi:10.1016/j.febslet.2010.04.028
Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation.
Goult, B. T., Bouaouina, M., Elliott, P. R., Bate, N., Patel, B., Gingras, A. R., . . . Barsukov, I. L. (2010). Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation.. The EMBO journal, 29(6), 1069-1080. doi:10.1038/emboj.2010.4
Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation
Goult, B. J., Bouaouina, M., Elliott, P. R., Bate, N., Patel, B., Gingras, A. R., . . . Barsukov, I. L. (2010). Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation. EMBO Journal, 29(6), 1069-1080.
2009
Domain motion in cytochrome P450 reductase: conformational equilibria revealed by NMR and small-angle x-ray scattering.
Ellis, J., Gutierrez, A., Barsukov, I. L., Huang, W. -C., Grossmann, J. G., & Roberts, G. C. K. (2009). Domain motion in cytochrome P450 reductase: conformational equilibria revealed by NMR and small-angle x-ray scattering.. The Journal of biological chemistry, 284(52), 36628-36637. doi:10.1074/jbc.m109.054304
The structure of the N-terminus of kindlin-1: a domain important for alphaiibbeta3 integrin activation.
Goult, B. T., Bouaouina, M., Harburger, D. S., Bate, N., Patel, B., Anthis, N. J., . . . Critchley, D. R. (2009). The structure of the N-terminus of kindlin-1: a domain important for alphaiibbeta3 integrin activation.. Journal of molecular biology, 394(5), 944-956. doi:10.1016/j.jmb.2009.09.061
The structure of an interdomain complex that regulates talin activity.
Goult, B. T., Bate, N., Anthis, N. J., Wegener, K. L., Gingras, A. R., Patel, B., . . . Critchley, D. R. (2009). The structure of an interdomain complex that regulates talin activity.. The Journal of biological chemistry, 284(22), 15097-15106. doi:10.1074/jbc.m900078200
Structural determinants of integrin binding to the talin rod.
Gingras, A. R., Ziegler, W. H., Bobkov, A. A., Joyce, M. G., Fasci, D., Himmel, M., . . . Critchley, D. R. (2009). Structural determinants of integrin binding to the talin rod.. The Journal of biological chemistry, 284(13), 8866-8876. doi:10.1074/jbc.m805937200
'Structural determinants of integrin binding to the talin rod'
Gingras, A. R., Ziegler, W. H., Bobkov, A. A., Joyce, M. G., Fasci, D., Himmel, M., . . . Critchley, D. R. (2009). 'Structural determinants of integrin binding to the talin rod'. The journal of Biological Chemistry, 284(13), 8866-8876.
2008
Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 subdomain.
Wegener, K. L., Basran, J., Bagshaw, C. R., Campbell, I. D., Roberts, G. C. K., Critchley, D. R., & Barsukov, I. L. (2008). Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 subdomain.. Journal of molecular biology, 382(1), 112-126. doi:10.1016/j.jmb.2008.06.087
NMR assignment of the C-terminal actin-binding domain of talin.
Goult, B. T., Gingras, A. R., Bate, N., Roberts, G. C. K., Critchley, D. R., & Barsukov, I. L. (2008). NMR assignment of the C-terminal actin-binding domain of talin.. Biomolecular NMR assignments, 2(1), 17-19. doi:10.1007/s12104-007-9073-5
Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4.
Gingras, A. R., Basran, J., Prescott, A., Kriajevska, M., Bagshaw, C. R., & Barsukov, I. L. (2008). Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4.. FEBS letters, 582(12), 1651-1656. doi:10.1016/j.febslet.2008.04.017
The structure of the C-terminal actin-binding domain of talin
Gingras, A. R., Bate, N., Goult, B. T., Hazelwood, L., Canestrelli, I., Grossmann, J. G., . . . Critchley, D. R. (2008). The structure of the C-terminal actin-binding domain of talin. The EMBO Journal, 27(2), 458-469. doi:10.1038/sj.emboj.7601965
The structure of the C-terminal actin-binding domain of talin.
Gingras, A. R., Bate, N., Goult, B. T., Hazelwood, L., Canestrelli, I., Grossmann, J. G., . . . Critchley, D. R. (2008). The structure of the C-terminal actin-binding domain of talin.. The EMBO journal, 27(2), 458-469. doi:10.1038/sj.emboj.7601965
2006
Role of vinculin in regulating focal adhesion turnover.
Saunders, R. M., Holt, M. R., Jennings, L., Sutton, D. H., Barsukov, I. L., Bobkov, A., . . . Critchley, D. R. (2006). Role of vinculin in regulating focal adhesion turnover.. European journal of cell biology, 85(6), 487-500. doi:10.1016/j.ejcb.2006.01.014
The activity of the vinculin binding sites in talin is influenced by the stability of the helical bundles that make up the talin rod.
Patel, B., Gingras, A. R., Bobkov, A. A., Fujimoto, L. M., Zhang, M., Liddington, R. C., . . . Critchley, D. R. (2006). The activity of the vinculin binding sites in talin is influenced by the stability of the helical bundles that make up the talin rod.. The Journal of biological chemistry, 281(11), 7458-7467. doi:10.1074/jbc.m508058200
Structural and dynamic characterization of a vinculin binding site in the talin rod.
Gingras, A. R., Vogel, K. -P., Steinhoff, H. -J., Ziegler, W. H., Patel, B., Emsley, J., . . . Barsukov, I. L. (2006). Structural and dynamic characterization of a vinculin binding site in the talin rod.. Biochemistry, 45(6), 1805-1817. doi:10.1021/bi052136l
2005
Mapping and consensus sequence identification for multiple vinculin binding sites within the talin rod.
Gingras, A. R., Ziegler, W. H., Frank, R., Barsukov, I. L., Roberts, G. C. K., Critchley, D. R., & Emsley, J. (2005). Mapping and consensus sequence identification for multiple vinculin binding sites within the talin rod.. The Journal of biological chemistry, 280(44), 37217-37224. doi:10.1074/jbc.m508060200
Reaction of morphinone reductase with 2-cyclohexen-1-one and 1-nitrocyclohexene: proton donation, ligand binding, and the role of residues Histidine 186 and Asparagine 189.
Messiha, H. L., Munro, A. W., Bruce, N. C., Barsukov, I., & Scrutton, N. S. (2005). Reaction of morphinone reductase with 2-cyclohexen-1-one and 1-nitrocyclohexene: proton donation, ligand binding, and the role of residues Histidine 186 and Asparagine 189.. The Journal of biological chemistry, 280(11), 10695-10709. doi:10.1074/jbc.m410595200
A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head.
Fillingham, I., Gingras, A. R., Papagrigoriou, E., Patel, B., Emsley, J., Critchley, D. R., . . . Barsukov, I. L. (2005). A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head.. Structure (London, England : 1993), 13(1), 65-74. doi:10.1016/j.str.2004.11.006
2004
Structural studies of the talin VBS3 with vinculin head
Gingras, A. R., Papagrigoriou, E., Barsukov, I., Critchley, D. R., & Emsley, J. (2004). Structural studies of the talin VBS3 with vinculin head. Acta Crystallographica Section A Foundations of Crystallography, 60(a1), s149. doi:10.1107/s0108767304097065
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle.
Papagrigoriou, E., Gingras, A. R., Barsukov, I. L., Bate, N., Fillingham, I. J., Patel, B., . . . Emsley, J. (2004). Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle.. The EMBO journal, 23(15), 2942-2951. doi:10.1038/sj.emboj.7600285
Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degradation.
Khan, H., Barna, T., Harris, R. J., Bruce, N. C., Barsukov, I., Munro, A. W., . . . Scrutton, N. S. (2004). Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degradation.. The Journal of biological chemistry, 279(29), 30563-30572. doi:10.1074/jbc.m403541200
A stereodivergent asymmetric approach to difluorinated aldonic acids.
Audouard, C., Barsukov, I., Fawcett, J., Griffith, G. A., Percy, J. M., Pintat, S., & Smith, C. A. (2004). A stereodivergent asymmetric approach to difluorinated aldonic acids.. Chemical communications (Cambridge, England), (13), 1526-1527. doi:10.1039/b405067c
Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands.
Day, C. L., Puthalakath, H., Skea, G., Strasser, A., Barsukov, I., Lian, L. -Y., . . . Hinds, M. G. (2004). Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands.. The Biochemical journal, 377(Pt 3), 597-605. doi:10.1042/bj20031251
2003
Phosphatidylinositol phosphate kinase type 1gamma and beta1-integrin cytoplasmic domain bind to the same region in the talin FERM domain.
Barsukov, I. L., Prescot, A., Bate, N., Patel, B., Floyd, D. N., Bhanji, N., . . . Critchley, D. R. (2003). Phosphatidylinositol phosphate kinase type 1gamma and beta1-integrin cytoplasmic domain bind to the same region in the talin FERM domain.. The Journal of biological chemistry, 278(33), 31202-31209. doi:10.1074/jbc.m303850200
The inhibitor thiomandelic acid binds to both metal ions in metallo-beta-lactamase and induces positive cooperativity in metal binding.
Damblon, C., Jensen, M., Ababou, A., Barsukov, I., Papamicael, C., Schofield, C. J., . . . Roberts, G. C. K. (2003). The inhibitor thiomandelic acid binds to both metal ions in metallo-beta-lactamase and induces positive cooperativity in metal binding.. The Journal of biological chemistry, 278(31), 29240-29251. doi:10.1074/jbc.m301562200
2002
Further characterization of the interaction between the cytoskeletal proteins talin and vinculin
BASS, M. D., PATEL, B., BARSUKOV, I. G., FILLINGHAM, I. J., MASON, R., SMITH, B. J., . . . CRITCHLEY, D. R. (2002). Further characterization of the interaction between the cytoskeletal proteins talin and vinculin. Biochemical Journal, 362(3), 761-768. doi:10.1042/bj3620761
2001
Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme.
Barna, T. M., Khan, H., Bruce, N. C., Barsukov, I., Scrutton, N. S., & Moody, P. C. (2001). Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme.. Journal of molecular biology, 310(2), 433-447. doi:10.1006/jmbi.2001.4779
Structural consequences of site-directed mutagenesis in flexible protein domains: NMR characterization of the L(55,56)S mutant of RhoGDI.
Golovanov, A. P., Hawkins, D., Barsukov, I., Badii, R., Bokoch, G. M., Lian, L. Y., & Roberts, G. C. (2001). Structural consequences of site-directed mutagenesis in flexible protein domains: NMR characterization of the L(55,56)S mutant of RhoGDI.. European journal of biochemistry, 268(8), 2253-2260. doi:10.1046/j.1432-1327.2001.02100.x
Structure-activity relationships in flexible protein domains: regulation of rho GTPases by RhoGDI and D4 GDI.
Golovanov, A. P., Chuang, T. H., DerMardirossian, C., Barsukov, I., Hawkins, D., Badii, R., . . . Roberts, G. C. (2001). Structure-activity relationships in flexible protein domains: regulation of rho GTPases by RhoGDI and D4 GDI.. Journal of molecular biology, 305(1), 121-135. doi:10.1006/jmbi.2000.4262
Structural consequences of site-directed mutagenesis in flexible protein domains: NMR characterization of the L(55,56)S mutant of RhoGDI
Golovanov, A. P., Hawkins, D., Barsukov, I., Badii, R., Bokoch, G. M., Lian, L. Y., & Roberts, G. C. (2001). Structural consequences of site-directed mutagenesis in flexible protein domains: NMR characterization of the L(55,56)S mutant of RhoGDI. Eur J Biochem, 268(8), 2253-2260. Retrieved from http://www.ncbi.nlm.nih.gov/
2000
Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1.
Lian, L. Y., Barsukov, I., Golovanov, A. P., Hawkins, D. I., Badii, R., Sze, K. H., . . . Roberts, G. C. (2000). Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1.. Structure (London, England : 1993), 8(1), 47-55. doi:10.1016/s0969-2126(00)00080-0
1999
<sup>1</sup>H−<sup>15</sup>N HMQC for the Identification of Metal-Bound Histidines in<sup>113</sup>Cd-Substituted<i>Bacillus cereus</i>Zinc β-Lactamase
Damblon, C., Prosperi, C., Lian, L. -Y., Barsukov, I., Soto, R. P., Galleni, M., . . . Roberts, G. C. K. (1999). <sup>1</sup>H−<sup>15</sup>N HMQC for the Identification of Metal-Bound Histidines in<sup>113</sup>Cd-Substituted<i>Bacillus cereus</i>Zinc β-Lactamase. Journal of the American Chemical Society, 121(49), 11575-11576. doi:10.1021/ja992896h
Rho-GDI - Rac interaction by NMR
Barsukov, I., Lian, L. -Y., Badii, R., Sze, K. -H., & Roberts, G. C. K. (1999). Rho-GDI - Rac interaction by NMR. Biochemical Society Transactions, 27(1), A37. doi:10.1042/bst027a037b
1998
The solution structure of the complex of Lactobacillus casei dihydrofolate reductase with methotrexate.
Gargaro, A. R., Soteriou, A., Frenkiel, T. A., Bauer, C. J., Birdsall, B., Polshakov, V. I., . . . Feeney, J. (1998). The solution structure of the complex of Lactobacillus casei dihydrofolate reductase with methotrexate.. Journal of molecular biology, 277(1), 119-134. doi:10.1006/jmbi.1997.1560
1997
1H, 15N and 13C NMR resonance assignment, secondary structure and global fold of the FMN-binding domain of human cytochrome P450 reductase
Barsukov, I., Modi, S., Lian, L. Y., Sze, K. H., Paine, M. J., Wolf, C. R., & Roberts, G. C. (1997). 1H, 15N and 13C NMR resonance assignment, secondary structure and global fold of the FMN-binding domain of human cytochrome P450 reductase. J Biomol NMR, 10(1), 63-75. Retrieved from http://www.ncbi.nlm.nih.gov/
A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm.
Keep, N. H., Barnes, M., Barsukov, I., Badii, R., Lian, L. Y., Segal, A. W., . . . Roberts, G. C. (1997). A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm.. Structure (London, England : 1993), 5(5), 623-633. doi:10.1016/s0969-2126(97)00218-9
H-1,N-15 and C-13 NMR resonance assignment, secondary structure and global fold of the FMN-binding domain of human cytochrome P450 reductase
Barsukov, I., Modi, S., Lian, L. Y., Sze, K. H., Paine, M. J. I., Wolf, C. R., & Roberts, G. C. K. (1997). H-1,N-15 and C-13 NMR resonance assignment, secondary structure and global fold of the FMN-binding domain of human cytochrome P450 reductase. JOURNAL OF BIOMOLECULAR NMR, 10(1), 63-75. doi:10.1023/A:1018313830207
1996
The conformation of coenzyme A bound to chloramphenicol acetyltransferase determined by transferred NOE experiments.
Barsukov, I. L., Lian, L. Y., Ellis, J., Sze, K. H., Shaw, W. V., & Roberts, G. C. (1996). The conformation of coenzyme A bound to chloramphenicol acetyltransferase determined by transferred NOE experiments.. Journal of molecular biology, 262(4), 543-558. doi:10.1006/jmbi.1996.0534
1995
Quantitative Evaluation of Cross-Peak Volumes in Multidimensional Spectra by Nonlinear-Least-Squares Curve Fitting
Sze, K. H., Barsukov, I. L., & Roberts, G. C. K. (1995). Quantitative Evaluation of Cross-Peak Volumes in Multidimensional Spectra by Nonlinear-Least-Squares Curve Fitting. Journal of Magnetic Resonance, Series A, 113(2), 185-195. doi:10.1006/jmra.1995.1079
Role of the active-site carboxylate in dihydrofolate reductase: kinetic and spectroscopic studies of the aspartate 26-->asparagine mutant of the Lactobacillus casei enzyme.
Basran, J., Casarotto, M. G., Barsukov, I. L., & Roberts, G. C. (1995). Role of the active-site carboxylate in dihydrofolate reductase: kinetic and spectroscopic studies of the aspartate 26-->asparagine mutant of the Lactobacillus casei enzyme.. Biochemistry, 34(9), 2872-2882. doi:10.1021/bi00009a018
Model for the complex between protein G and an antibody Fc fragment in solution.
Kato, K., Lian, L. Y., Barsukov, I. L., Derrick, J. P., Kim, H., Tanaka, R., . . . Arata, Y. (1995). Model for the complex between protein G and an antibody Fc fragment in solution.. Structure (London, England : 1993), 3(1), 79-85. doi:10.1016/s0969-2126(01)00136-8
Interactions of bacterial cell-surface proteins with antibodies: a ersatile set of protein-protein interactions
Roberts, G. C. K., Lian, L. -Y., Barsukov, I. L., Derrick, J. P., Kato, K., & Arata, Y. (1995). Interactions of bacterial cell-surface proteins with antibodies: a ersatile set of protein-protein interactions. In Techniques in Protein Chemistry (pp. 409-416). Elsevier. doi:10.1016/s1080-8914(06)80050-5
1994
Mapping the interactions between streptococcal protein G and the Fab fragment of IgG in solution.
Lian, L. Y., Barsukov, I. L., Derrick, J. P., & Roberts, G. C. (1994). Mapping the interactions between streptococcal protein G and the Fab fragment of IgG in solution.. Nature structural biology, 1(6), 355-357. doi:10.1038/nsb0694-355
Protein-ligand interactions: exchange processes and determination of ligand conformation and protein-ligand contacts
Lian, L. Y., Barsukov, I. L., Sutcliffe, M. J., Sze, K. H., & Roberts, G. C. (1994). Protein-ligand interactions: exchange processes and determination of ligand conformation and protein-ligand contacts. Methods Enzymol, 239, 657-700. Retrieved from http://www.ncbi.nlm.nih.gov/
Protein-ligand interactions: exchange processes and determination of ligand conformation and protein-ligand contacts.
Lian, L. Y., Barsukov, I. L., Sutcliffe, M. J., Sze, K. H., & Roberts, G. C. (1994). Protein-ligand interactions: exchange processes and determination of ligand conformation and protein-ligand contacts.. In Unknown Book (Vol. 239, pp. 657-700). doi:10.1016/s0076-6879(94)39025-8
1992
Evaluation of cross-peak volumes in 2D-spectra using 1D-fitting of line shapes
Barsukov, I. L., Pervushin, K. V., Orekhov, V. Y., Barsukov, T. L., & Arseniev, A. S. (1992). Evaluation of cross-peak volumes in 2D-spectra using 1D-fitting of line shapes. Applied Magnetic Resonance, 3(6), 965-973. doi:10.1007/bf03166166
Three-dimensional structure of proteolytic fragment 163-231 of bacterioopsin determined from nuclear magnetic resonance data in solution.
Barsukov, I. L., Nolde, D. E., Lomize, A. L., & Arseniev, A. S. (1992). Three-dimensional structure of proteolytic fragment 163-231 of bacterioopsin determined from nuclear magnetic resonance data in solution.. European journal of biochemistry, 206(3), 665-672. doi:10.1111/j.1432-1033.1992.tb16972.x
1991
ChemInform Abstract: 2D‐NMR for 3D‐Structure of Membrane Spanning Polypeptides: Gramacidin A and Fragments of Bacteriorhodopsin
BYSTROV, V. F., ARSEN'EV, A. S., BARSUKOV, I. L., LOMIZE, A. L., ABDULAEVA, G. V., SOBOL, A. G., . . . GOLOVANOV, A. P. (1991). ChemInform Abstract: 2D‐NMR for 3D‐Structure of Membrane Spanning Polypeptides: Gramacidin A and Fragments of Bacteriorhodopsin. ChemInform, 22(41). doi:10.1002/chin.199141314
The divalent cation-binding sites of gramicidin A transmembrane ion-channel.
Golovanov, A. P., Barsukov, I. L., Arseniev, A. S., Bystrov, V. F., Sukhanov, S. V., & Barsukov, L. I. (1991). The divalent cation-binding sites of gramicidin A transmembrane ion-channel.. Biopolymers, 31(4), 425-434. doi:10.1002/bip.360310408
1990
Sequence-specific 1H-NMR assignment and conformation of proteolytic fragment 163-231 of bacterioopsin.
Barsukov, I. L., Abdulaeva, G. V., Arseniev, A. S., & Bystrov, V. F. (1990). Sequence-specific 1H-NMR assignment and conformation of proteolytic fragment 163-231 of bacterioopsin.. European journal of biochemistry, 192(2), 321-327. doi:10.1111/j.1432-1033.1990.tb19230.x
1989
2D-NMR for 3D-Structure of Membrane Spanning Polypeptides: Gramacidin A and Fragments of Bacteriorhodopsin
Bystrov, V. F., Arseniev, A. S., Barsukov, I. L., Lomize, A. L., Abdulaeva, G. V., Sobol, A. G., . . . Golovanov, A. P. (1989). 2D-NMR for 3D-Structure of Membrane Spanning Polypeptides: Gramacidin A and Fragments of Bacteriorhodopsin. In Protein Structure and Engineering (pp. 111-138). Springer US. doi:10.1007/978-1-4684-5745-2_9
1987
ChemInform Abstract: The 2D NMR of Single and Double Stranded Helixes of Gramicidin A in Micelles and Solutions
BYSTROV, V. F., ARSEN'EV, A. S., BARSUKOV, I. L., & LOMIZE, A. L. (1987). ChemInform Abstract: The 2D NMR of Single and Double Stranded Helixes of Gramicidin A in Micelles and Solutions. ChemInform, 18(52). doi:10.1002/chin.198752400
[Spatial structure of gramicidin A in organic solvents. 1H-NMR analysis of conformation heterogeneity in ethanol].
Barsukov, I. L., Arsen'ev, A. S., & Bystrov, V. F. (1987). [Spatial structure of gramicidin A in organic solvents. 1H-NMR analysis of conformation heterogeneity in ethanol].. Bioorganicheskaia khimiia, 13(11), 1501-1522.
Base-plane correction in 2D NMR
Barsukov, I. L., & Arseniev, A. S. (1987). Base-plane correction in 2D NMR. Journal of Magnetic Resonance (1969), 73(1), 148-149. doi:10.1016/0022-2364(87)90234-4
1986
Conformation of Gramicidin A in Solution and Micelles: Two Dimensional H NMR Study
Arseniev, A. S., Barsukov, I. L., & Bystrov, V. F. (1986). Conformation of Gramicidin A in Solution and Micelles: Two Dimensional H NMR Study. In Proceedings of the Fifth USSR-FRG Symposium on Chemistry of Peptides and Proteins, Odessa, USSR, May 16–20, 1985 (pp. 127-158). De Gruyter. doi:10.1515/9783110858846-016
1985
1H-NMR study of gramicidin A transmembrane ion channel. Head-to-head right-handed, single-stranded helices.
Arseniev, A. S., Barsukov, I. L., Bystrov, V. F., Lomize, A. L., & Ovchinnikov YuA. (1985). 1H-NMR study of gramicidin A transmembrane ion channel. Head-to-head right-handed, single-stranded helices.. FEBS letters, 186(2), 168-174. doi:10.1016/0014-5793(85)80702-x
NMR solution structure of gramicidin A complex with caesium cations
Arseniev, A. S., Barsukov, I. L., & Bystrov, V. F. (1985). NMR solution structure of gramicidin A complex with caesium cations. FEBS Letters, 180(1), 33-39. doi:10.1016/0014-5793(85)80225-8
1984
Combined COSY-NOESY experiment
Gurevich, A. Z., Barsukov, I. L., Arseniev, A. S., & Bystrov, V. F. (1984). Combined COSY-NOESY experiment. Journal of Magnetic Resonance (1969), 56(3), 471-478. doi:10.1016/0022-2364(84)90311-1