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Hannah Levis

Dr Hannah Levis
PhD FHEA

Reader in Ophthalmic Bioengineering
Eye & Vision Sciences
Part of
Institute of Life Course & Medical Sciences
Faculty of Health and Life Sciences

Contact

H.Levis@liverpool.ac.uk

Publications

Selected publications

  1. Rapid tissue engineering of biomimetic human corneal limbal crypts with 3D niche architecture (Journal article - 2013)
  2. Plastic compressed collagen as a biomimetic substrate for human limbal epithelial cell culture (Journal article - 2010)
  3. A comparative study on different Descemet membrane endothelial keratoplasty graft preparation techniques (Journal article - 2018)
  4. Poly-ε-lysine based hydrogels as synthetic substrates for the expansion of corneal endothelial cells for transplantation (Journal article - 2019)
  5. A human retinal microvascular endothelial-pericyte co-culture model to study diabetic retinopathy in vitro (Journal article - 2020)
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2024

Quality assurance in corneal transplants: Donor cornea assessment and oversight.

Romano, V., Passaro, M. L., Ruzza, A., Parekh, M., Airaldi, M., Levis, H. J., . . . Ponzin, D. (2024). Quality assurance in corneal transplants: Donor cornea assessment and oversight.. Survey of ophthalmology, 69(3), 465-482. doi:10.1016/j.survophthal.2023.12.002

DOI
10.1016/j.survophthal.2023.12.002
Journal article

How endothelial keratoplasty has revolutionized current treatment of endothelial disease

Romano, V., & Levis, H. J. (2024). How endothelial keratoplasty has revolutionized current treatment of endothelial disease. Expert Review of Ophthalmology, 19(2), 87-88. doi:10.1080/17469899.2024.2305433

DOI
10.1080/17469899.2024.2305433
Journal article

Biofabrication and biomanufacturing in Ireland and the UK

Murphy, J. F., Lavelle, M., Asciak, L., Burdis, R., Levis, H. J., Ligorio, C., . . . Huang, Y. Y. S. (n.d.). Biofabrication and biomanufacturing in Ireland and the UK. Bio-Design and Manufacturing. doi:10.1007/s42242-024-00316-z

DOI
10.1007/s42242-024-00316-z
Journal article

2023

Preoperative surgeon evaluation of corneal endothelial status: the Viability Control of Human Endothelial Cells before Keratoplasty (V-CHECK) study protocol.

Airaldi, M., Zheng, Y., Aiello, F., Bachmann, B., Baydoun, L., Ní Dhubhghaill, S., . . . Romano, V. (2023). Preoperative surgeon evaluation of corneal endothelial status: the Viability Control of Human Endothelial Cells before Keratoplasty (V-CHECK) study protocol.. BMJ open ophthalmology, 8(1), e001361. doi:10.1136/bmjophth-2023-001361

DOI
10.1136/bmjophth-2023-001361
Journal article

P27-A149 Liverpool research eye biobank-our exerience of expanding to collect from living donors.

Simon, S., Mount, L., Moss, S., Kaye, S. B., & Levis, H. J. (2023). P27-A149 Liverpool research eye biobank-our exerience of expanding to collect from living donors.. BMJ open ophthalmology, 8(Suppl 2), A11-A12. doi:10.1136/bmjophth-2023-eeba.26

DOI
10.1136/bmjophth-2023-eeba.26
Journal article

Incidence and management of early postoperative complications in lamellar corneal transplantation.

Romano, D., Aiello, F., Parekh, M., Levis, H. J., Gadhvi, K. A., Moramarco, A., . . . Romano, V. (2023). Incidence and management of early postoperative complications in lamellar corneal transplantation.. Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. doi:10.1007/s00417-023-06073-6

DOI
10.1007/s00417-023-06073-6
Journal article

DMEK graft: One size does not fit all

Parekh, M., Romano, D., Wongvisavavit, R., Coco, G., Giannaccare, G., Ferrari, S., . . . Romano, V. (2023). DMEK graft: One size does not fit all. ACTA OPHTHALMOLOGICA, 101(1), E14-E25. doi:10.1111/aos.15202

DOI
10.1111/aos.15202
Journal article

Developing new imaging tools to understand laminin network formation and remodelling

Chavda, N., Sugden, C., Levis, H., Jahoda, C., & Hamill, K. (2023). Developing new imaging tools to understand laminin network formation and remodelling. In INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY Vol. 104 (pp. A13-A14). Retrieved from https://www.webofscience.com/

Conference Paper

Development of a biosynthetic corneal endothelial grafts to alleviate donor corneal shortage

Robinson, D., Bilir, E., Romano, V., Gadhvi, K., Williams, R., & Levis, H. (2023). Development of a biosynthetic corneal endothelial grafts to alleviate donor corneal shortage. In TISSUE ENGINEERING PART A Vol. 29. Retrieved from https://www.webofscience.com/

Conference Paper

Non-invasive infrared spectroscopy of aqueous humour samples in glaucoma compared to controls

Vallabh, N. A., Lima, C., Levis, H., Muntasser, H., Kaye, S., & Goodacre, R. (2023). Non-invasive infrared spectroscopy of aqueous humour samples in glaucoma compared to controls. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 64. Retrieved from https://www.webofscience.com/

Conference Paper

2022

Eye bank versus surgeon prepared Descemet stripping automated endothelial keratoplasty tissues: Influence on adhesion force in a pilot study.

Romano, V., Parekh, M., Kazaili, A., Steger, B., Akhtar, R., Ferrari, S., . . . Levis, H. J. (2022). Eye bank versus surgeon prepared Descemet stripping automated endothelial keratoplasty tissues: Influence on adhesion force in a pilot study. INDIAN JOURNAL OF OPHTHALMOLOGY, 70(2), 523-528. doi:10.4103/ijo.IJO_3637_20

DOI
10.4103/ijo.ijo_3637_20
Journal article

Corneal Endothelial Cell Loss in Glaucoma and Glaucoma Surgery and the Utility of Management with Descemet Membrane Endothelial Keratoplasty (DMEK)

Vallabh, N. A., Kennedy, S., Vinciguerra, R., McLean, K., Levis, H., Borroni, D., . . . Willoughby, C. E. (2022). Corneal Endothelial Cell Loss in Glaucoma and Glaucoma Surgery and the Utility of Management with Descemet Membrane Endothelial Keratoplasty (DMEK). Journal of Ophthalmology, 2022, 1-17. doi:10.1155/2022/1315299

DOI
10.1155/2022/1315299
Journal article

2021

Long-Term Outcome of Same-Day Split Corneal Transplantation in Hong Kong Reply

Gadhvi, K. A., Coco, G., Pagano, L., Kaye, S. B., Ferrari, S., Levis, H. J., . . . Romano, V. (2021). Long-Term Outcome of Same-Day Split Corneal Transplantation in Hong Kong Reply. CORNEA, 40(9), E16-E17. Retrieved from https://www.webofscience.com/

Journal article

2020

DSAEK Centration and Interface Folds: Surgical Management

Gadhvi, K., Pagano, L., Menassa, N., Borroni, D., Kaye, S. B., Levis, H. J., & Romano, V. (2020). DSAEK Centration and Interface Folds: Surgical Management. CORNEA, 39(11), 1457-1459. doi:10.1097/ICO.0000000000002411

DOI
10.1097/ICO.0000000000002411
Journal article

2019

Strategies to improve corneal endothelial graft outcomes

Levis, H. (2019). Strategies to improve corneal endothelial graft outcomes. In Acta Ophthalmologica Vol. 97. Nice: Blackwell Publishing Inc.. doi:10.1111/j.1755-3768.2019.8107

DOI
10.1111/j.1755-3768.2019.8107
Conference Paper

Development of an ex vivo organ culture model of the cornea: a useful tool for corneal studies

Carserides, C., Williams, R., Romano, V., & Levis, H. J. (2019). Development of an ex vivo organ culture model of the cornea: a useful tool for corneal studies. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 60. Retrieved from https://www.webofscience.com/

Conference Paper

Endothelial colony forming cell repair in an in vitro co-culture model of the diabetic human retinal microvasculature

Levis, H. J., Williams, R., & Eyre, J. J. (2019). Endothelial colony forming cell repair in an <i>in vitro</i> co-culture model of the diabetic human retinal microvasculature. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 60. Retrieved from https://www.webofscience.com/

Conference Paper

Biomaterials in Ophthalmology

Williams, R. L., Levis, H. J., Lace, R., Doherty, K. G., Kennedy, S. M., & Kearns, V. R. (2019). Biomaterials in Ophthalmology. In ENCYCLOPEDIA OF BIOMEDICAL ENGINEERING, VOL 1 (pp. 289-300). doi:10.1016/B978-0-12-801238-3.11034-7

DOI
10.1016/B978-0-12-801238-3.11034-7
Chapter

INCORPORATION OF ENDOTHELIAL COLONY FORMING CELLS INTO AN IN VITRO HUMAN PRIMARY CO-CULTURE MODEL OF DIABETIC RETINOPATHY

Eyre, J. J., Bosworth, L. A., Williams, R. L., & Levis, H. J. (2019). INCORPORATION OF ENDOTHELIAL COLONY FORMING CELLS INTO AN IN VITRO HUMAN PRIMARY CO-CULTURE MODEL OF DIABETIC RETINOPATHY. In EUROPEAN JOURNAL OF OPHTHALMOLOGY Vol. 29 (pp. NP21-NP22). Retrieved from https://www.webofscience.com/

Conference Paper

Nanotopography of substrates directs the deposition of fibrillar collagen by corneal stromal cells and deposition is accelerated by macromolecular crowding

O'Loughlin, D. A., Kearns, V. R., Levis, H. J., Sheridan, C., & Canty-Laird, E. G. (2019). Nanotopography of substrates directs the deposition of fibrillar collagen by corneal stromal cells and deposition is accelerated by macromolecular crowding. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 60. Retrieved from https://www.webofscience.com/

Conference Paper

2018

Comparison of preservation and transportation protocols for preloaded Descemet membrane endothelial keratoplasty

Romano, V., Parekh, M., Ruzza, A., Willoughby, C. E., Ferrari, S., Ponzin, D., . . . Levis, H. J. (2018). Comparison of preservation and transportation protocols for preloaded Descemet membrane endothelial keratoplasty. BRITISH JOURNAL OF OPHTHALMOLOGY, 102(4), 549-555. doi:10.1136/bjophthalmol-2017-310906

DOI
10.1136/bjophthalmol-2017-310906
Journal article

An <i>in vitro</i> co-culture model of the human retinal vasculature for use in development of regenerative medicine strategies for diabetic retinopathy.

Eyre, J. J., Williams, R., & Levis, H. J. (2018). An <i>in vitro</i> co-culture model of the human retinal vasculature for use in development of regenerative medicine strategies for diabetic retinopathy.. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 59. Retrieved from https://www.webofscience.com/

Conference Paper

2016

Recreating the Human Limbal Epithelial Stem Cell Niche with Bioengineered Limbal Crypts

Levis, H. J., & Daniels, J. T. (2016). Recreating the Human Limbal Epithelial Stem Cell Niche with Bioengineered Limbal Crypts. CURRENT EYE RESEARCH, 41(9), 1153-1160. doi:10.3109/02713683.2015.1095932

DOI
10.3109/02713683.2015.1095932
Journal article

Autosomal-Dominant Corneal Endothelial Dystrophies CHED1 and PPCD1 Are Allelic Disorders Caused by Non-coding Mutations in the Promoter of OVOL2

Davidson, A. E., Liskova, P., Evans, C. J., Dudakova, L., Noskova, L., Pontikos, N., . . . Hardcastle, A. J. (2016). Autosomal-Dominant Corneal Endothelial Dystrophies CHED1 and PPCD1 Are Allelic Disorders Caused by Non-coding Mutations in the Promoter of OVOL2. AMERICAN JOURNAL OF HUMAN GENETICS, 98(1), 75-89. doi:10.1016/j.ajhg.2015.11.018

DOI
10.1016/j.ajhg.2015.11.018
Journal article

2015

Tissue Engineering the Cornea: The Evolution of RAFT

Levis, H. J., Kureshi, A. K., Massie, I., Morgan, L., Vernon, A. J., & Daniels, J. T. (2015). Tissue Engineering the Cornea: The Evolution of RAFT. JOURNAL OF FUNCTIONAL BIOMATERIALS, 6(1), 50-65. doi:10.3390/jfb6010050

DOI
10.3390/jfb6010050
Journal article

2014

Response of human limbal epithelial cells to wounding on 3D RAFT tissue equivalents: Effect of airlifting and human limbal fibroblasts

Massie, I., Levis, H., & Daniels, J. T. (2014). Response of human limbal epithelial cells to wounding on 3D RAFT tissue equivalents: Effect of airlifting and human limbal fibroblasts. Experimental Eye Research, 127, 196-205. doi:10.1016/j.exer.2014.07.024

DOI
10.1016/j.exer.2014.07.024
Journal article

Localisation of Epithelial Cells Capable of Holoclone Formation In Vitro and Direct Interaction with Stromal Cells in the Native Human Limbal Crypt

Dziasko, M. A., Armer, H. E., Levis, H., Shortt, A. J., Tuft, S., & Daniels, J. T. (2014). Localisation of Epithelial Cells Capable of Holoclone Formation In Vitro and Direct Interaction with Stromal Cells in the Native Human Limbal Crypt. PLoS ONE, 9(4). doi:10.1371/journal.pone.0094283

DOI
10.1371/journal.pone.0094283
Journal article

Three-Year Outcomes of Cultured Limbal Epithelial Allografts in Aniridia and Stevens-Johnson Syndrome Evaluated Using the Clinical Outcome Assessment in Surgical Trials Assessment Tool

Shortt, A. J., Bunce, C., Levis, H., Blows, P., Dore, C. J., Vernon, A., . . . Daniels, J. T. (2014). Three-Year Outcomes of Cultured Limbal Epithelial Allografts in Aniridia and Stevens-Johnson Syndrome Evaluated Using the Clinical Outcome Assessment in Surgical Trials Assessment Tool. Stem Cells Translational Medicine, 3(2), 265-275. doi:10.5966/sctm.2013-0025

DOI
10.5966/sctm.2013-0025
Journal article

Advanced Imaging and Tissue Engineering of the Human Limbal Epithelial Stem Cell Niche

Massie, I., Dziasko, M., Kureshi, A., Levis, H. J., Morgan, L., Neale, M., . . . Daniels, J. T. (2015). Advanced Imaging and Tissue Engineering of the Human Limbal Epithelial Stem Cell Niche. STEM CELL PROTOCOLS, 1235, 179-202. doi:10.1007/978-1-4939-1785-3_15

DOI
10.1007/978-1-4939-1785-3_15
Journal article

Tissue engineering the human limbal crypts: further characterisation of an in vitro model

Levis, H. J., & Daniels, J. T. (2014). Tissue engineering the human limbal crypts: further characterisation of an in vitro model. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 55. Retrieved from https://www.webofscience.com/

Conference Paper

2013

Rapid tissue engineering of biomimetic human corneal limbal crypts with 3D niche architecture

Levis, H. J., Massie, I., Dziasko, M. A., Kaasi, A., & Daniels, J. T. (2013). Rapid tissue engineering of biomimetic human corneal limbal crypts with 3D niche architecture. BIOMATERIALS, 34(35), 8860-8868. doi:10.1016/j.biomaterials.2013.08.002

DOI
10.1016/j.biomaterials.2013.08.002
Journal article

Cellular cross-talk in the limbal epithelial stem cell niche revealed by high-resolution microscopy

Daniels, J., Armer, H., Levis, H., Munro, P., Shortt, A., Tuft, S., & Dziasko, M. (2013). Cellular cross-talk in the limbal epithelial stem cell niche revealed by high-resolution microscopy. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 54. Retrieved from https://www.webofscience.com/

Conference Paper

Cultured Limbal Epithelial Stem Cell Therapy for Ocular Surface Diseases

Levis, H. J., Daniels, J. T., & Ahmad, S. (2013). Cultured Limbal Epithelial Stem Cell Therapy for Ocular Surface Diseases. In Regenerative Medicine and Cell Therapy (pp. 41-56). Humana Press. doi:10.1007/978-1-62703-098-4_3

DOI
10.1007/978-1-62703-098-4_3
Chapter

Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss

Levis, H. J., Menzel-Severing, J., Drake, R. A. L., & Daniels, J. T. (2013). Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss. CURRENT EYE RESEARCH, 38(1), 41-52. doi:10.3109/02713683.2012.725799

DOI
10.3109/02713683.2012.725799
Journal article

2012

Plastic Compressed Collagen as a Novel Carrier for Expanded Human Corneal Endothelial Cells for Transplantation

Levis, H. J., Peh, G. S. L., Toh, K. -P., Poh, R., Shortt, A. J., Drake, R. A. L., . . . Daniels, J. T. (2012). Plastic Compressed Collagen as a Novel Carrier for Expanded Human Corneal Endothelial Cells for Transplantation. PLOS ONE, 7(11). doi:10.1371/journal.pone.0050993

DOI
10.1371/journal.pone.0050993
Journal article

A tissue-engineered plastic compressed collagen substrate suitable for human corneal endothelial layer transplantation

Levis, H. J., Peh, G. S. L., Toh, K. P., Poh, R. W. Y., Drake, R., Mehta, J. S., & Daniels, J. T. (2012). A tissue-engineered plastic compressed collagen substrate suitable for human corneal endothelial layer transplantation. In JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE Vol. 6 (pp. 136). Retrieved from https://www.webofscience.com/

Conference Paper

2011

Organotypic slices culture model for cerebellar ataxia: Potential use to study Purkinje cell induction from neural stem cells

Lu, H. -X., Levis, H., Liu, Y., & Parker, T. (2011). Organotypic slices culture model for cerebellar ataxia: Potential use to study Purkinje cell induction from neural stem cells. BRAIN RESEARCH BULLETIN, 84(2), 169-173. doi:10.1016/j.brainresbull.2010.12.001

DOI
10.1016/j.brainresbull.2010.12.001
Journal article

2010

Plastic compressed collagen as a biomimetic substrate for human limbal epithelial cell culture

Levis, H. J., Brown, R. A., & Daniels, J. T. (2010). Plastic compressed collagen as a biomimetic substrate for human limbal epithelial cell culture. BIOMATERIALS, 31(30), 7726-7737. doi:10.1016/j.biomaterials.2010.07.012

DOI
10.1016/j.biomaterials.2010.07.012
Journal article

In sickness and in health: Corneal epithelial stem cell biology, pathology and therapy

Notara, M., Alatza, A., Gilfillan, J., Harris, A. R., Levis, H. J., Schrader, S., . . . Daniels, J. T. (2010). In sickness and in health: Corneal epithelial stem cell biology, pathology and therapy. EXPERIMENTAL EYE RESEARCH, 90(2), 188-195. doi:10.1016/j.exer.2009.09.023

DOI
10.1016/j.exer.2009.09.023
Journal article

Plastic Compressed Collagen as a Novel Carrier for Cultured Limbal Epithelial Cells

Levis, H., Alekseeva, T., Jawad, H., Brown, R. A., Tully, F., & Daniels, J. T. (2010). Plastic Compressed Collagen as a Novel Carrier for Cultured Limbal Epithelial Cells. In INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE Vol. 51. Retrieved from https://www.webofscience.com/

Conference Paper

2009

New technologies in limbal epithelial stem cell transplantation

Levis, H., & Daniels, J. T. (2009). New technologies in limbal epithelial stem cell transplantation. CURRENT OPINION IN BIOTECHNOLOGY, 20(5), 593-597. doi:10.1016/j.copbio.2009.09.002

DOI
10.1016/j.copbio.2009.09.002
Journal article

Bergmann glia of the early postnatal rat cerebellum express stem cell markers and retain neural stem cell characteristics in neurospheroid cultures of comparable ages

Levis, H., Lu, H. -X., & Parker, T. L. (2009). Bergmann glia of the early postnatal rat cerebellum express stem cell markers and retain neural stem cell characteristics in neurospheroid cultures of comparable ages. In JOURNAL OF ANATOMY Vol. 214 (pp. 795-796). Retrieved from https://www.webofscience.com/

Conference Paper

2008

Toxin-produced Purkinje cell death: A model for neural stem cell transplantation studies

Lu, H. -X., Levis, H., Melhem, N., & Parker, T. (2008). Toxin-produced Purkinje cell death: A model for neural stem cell transplantation studies. BRAIN RESEARCH, 1207, 207-213. doi:10.1016/j.brainres.2008.02.034

DOI
10.1016/j.brainres.2008.02.034
Journal article

Cerebellar ataxia: the establishment of an animal model of Purkinje cell damage in the rat cerebellum suitable for neural stem cell transplantation

Lu, H. X., Levis, H., Melhem, N., & Parker, T. L. (2008). Cerebellar ataxia: the establishment of an animal model of Purkinje cell damage in the rat cerebellum suitable for neural stem cell transplantation. In JOURNAL OF ANATOMY Vol. 212 (pp. 90-91). Retrieved from https://www.webofscience.com/

Conference Paper

2004

Environmental enrichment results in cortical and subcortical changes in levels of synaptophysin and PSD-95 proteins.

Nithianantharajah, J., Levis, H., & Murphy, M. (2004). Environmental enrichment results in cortical and subcortical changes in levels of synaptophysin and PSD-95 proteins.. Neurobiology of learning and memory, 81(3), 200-210. doi:10.1016/j.nlm.2004.02.002

DOI
10.1016/j.nlm.2004.02.002
Journal article

Environmental enrichment results in cortical and subcortical changes in levels of synaptophysin and PSD-95 proteins

Nithianantharajah, J., Levis, H., & Murphy, M. (2004). Environmental enrichment results in cortical and subcortical changes in levels of synaptophysin and PSD-95 proteins. NEUROBIOLOGY OF LEARNING AND MEMORY, 81(3), 200-210. doi:10.1016/j.nlm.2004.02.002

DOI
10.1016/j.nlm.2004.02.002
Journal article