Publications
2024
What is ‘research-led teaching’ and how can we deliver it in University STEM degrees?
Canniffe, D., Cartmell, A., & McClurg, U. (2024). What is ‘research-led teaching’ and how can we deliver it in University STEM degrees?. Journal of Biological Education, 1-15. doi:10.1080/00219266.2024.2351389
2023
Common loss of far-red light photoacclimation in cyanobacteria from hot and cold deserts: a case study in the Chroococcidiopsidales.
Antonaru, L. A., Selinger, V. M., Jung, P., Di Stefano, G., Sanderson, N. D., Barker, L., . . . Nürnberg, D. J. (2023). Common loss of far-red light photoacclimation in cyanobacteria from hot and cold deserts: a case study in the Chroococcidiopsidales.. ISME communications, 3(1), 113. doi:10.1038/s43705-023-00319-4
Globally distributed Myxococcota with photosynthesis gene clusters illuminate the origin and evolution of a potentially chimeric lifestyle.
Li, L., Huang, D., Hu, Y., Rudling, N. M., Canniffe, D. P., Wang, F., & Wang, Y. (2023). Globally distributed Myxococcota with photosynthesis gene clusters illuminate the origin and evolution of a potentially chimeric lifestyle.. Nature communications, 14(1), 6450. doi:10.1038/s41467-023-42193-7
2022
The role of the γ subunit in the photosystem of the lowest-energy phototrophs.
Namoon, D., Rudling, N. M., & Canniffe, D. P. (2022). The role of the γ subunit in the photosystem of the lowest-energy phototrophs.. The Biochemical journal, 479(24), 2449-2463. doi:10.1042/bcj20220508
Zeta-Carotene Isomerase (Z-ISO) Is Required for Light-Independent Carotenoid Biosynthesis in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803
Proctor, M. S., Morey-Burrows, F. S., Canniffe, D. P., Martin, E. C., Swainsbury, D. J. K., Johnson, M. P., . . . Hitchcock, A. (2022). Zeta-Carotene Isomerase (Z-ISO) Is Required for Light-Independent Carotenoid Biosynthesis in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803. MICROORGANISMS, 10(9). doi:10.3390/microorganisms10091730
Structural basis for the assembly and quinone transport mechanisms of the dimeric photosynthetic RC-LH1 supercomplex
Cao, P., Bracun, L., Yamagata, A., Christianson, B. M., Negami, T., Zou, B., . . . Liu, L. -N. (2022). Structural basis for the assembly and quinone transport mechanisms of the dimeric photosynthetic RC-LH1 supercomplex. NATURE COMMUNICATIONS, 13(1). doi:10.1038/s41467-022-29563-3
The terminal enzymes of (bacterio)chlorophyll biosynthesis.
Proctor, M. S., Sutherland, G. A., Canniffe, D. P., & Hitchcock, A. (2022). The terminal enzymes of (bacterio)chlorophyll biosynthesis.. Royal Society open science, 9(5), 211903. doi:10.1098/rsos.211903
2021
Photosynthesis | Carotenoids in Photosynthesis – Structure and Biosynthesis
Canniffe, D. P., & Hitchcock, A. (2021). Photosynthesis | Carotenoids in Photosynthesis – Structure and Biosynthesis. In Encyclopedia of Biological Chemistry III (pp. 163-185). Elsevier. doi:10.1016/b978-0-12-819460-7.00087-6
Cryo-EM structure of the photosynthetic RC-LH1-PufX supercomplex at 2.8-Å resolution
Bracun, L., Yamagata, A., Christianson, B. M., Terada, T., Canniffe, D. P., Shirouzu, M., & Liu, L. -N. (2021). Cryo-EM structure of the photosynthetic RC-LH1-PufX supercomplex at 2.8-Å resolution. SCIENCE ADVANCES, 7(25). doi:10.1126/sciadv.abf8864
Structures of Rhodopseudomonas palustris RC-LH1 complexes with open or closed quinone channels
Swainsbury, D. J. K., Qian, P., Jackson, P. J., Faries, K. M., Niedzwiedzki, D. M., Martin, E. C., . . . Hunter, C. N. (2021). Structures of <i>Rhodopseudomonas palustris</i> RC-LH1 complexes with open or closed quinone channels. SCIENCE ADVANCES, 7(3). doi:10.1126/sciadv.abe2631
2020
Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD
Proctor, M. S., Pazdernik, M., Jackson, P. J., Pilny, J., Martin, E. C., Dickman, M. J., . . . Hitchcock, A. (2020). Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD. BIOCHEMICAL JOURNAL, 477(20), 4021-4036. doi:10.1042/BCJ20200561
Unfolding pathway and intermolecular interactions of the cytochrome subunit in the bacterial photosynthetic reaction center
Miller, L. C., Zhao, L., Canniffe, D. P., Martin, D., & Liu, L. -N. (2020). Unfolding pathway and intermolecular interactions of the cytochrome subunit in the bacterial photosynthetic reaction center. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1861(8). doi:10.1016/j.bbabio.2020.148204
A photosynthetic antenna complex foregoes unity carotenoid-to-bacteriochlorophyll energy transfer efficiency to ensure photoprotection
Niedzwiedzki, D. M., Swainsbury, D. J. K., Canniffe, D. P., Hunter, C. N., & Hitchcock, A. (2020). A photosynthetic antenna complex foregoes unity carotenoid-to-bacteriochlorophyll energy transfer efficiency to ensure photoprotection. Proceedings of the National Academy of Sciences of USA, 117(12), 6502-6508. doi:10.1073/pnas.1920923117
Progress and challenges in engineering cyanobacteria as chassis for light-driven biotechnology
Hitchcock, A., Hunter, C. N., & Canniffe, D. P. (2020). Progress and challenges in engineering cyanobacteria as chassis for light-driven biotechnology. MICROBIAL BIOTECHNOLOGY, 13(2), 363-367. doi:10.1111/1751-7915.13526
Composition, Organisation and Function of Purple Photosynthetic Machinery
Miller, L. C., Martin, D. S., Liu, L. -N., & Canniffe, D. P. (2020). Composition, Organisation and Function of Purple Photosynthetic Machinery. In Microbial Photosynthesis (pp. 73-114). Springer Singapore. doi:10.1007/978-981-15-3110-1_4
2019
Engineering of B800 bacteriochlorophyll binding site specificity in the <i>Rhodobacter sphaeroides</i> LH2 antenna
Swainsbury, D. J. K., Faries, K. M., Niedzwiedzki, D. M., Martin, E. C., Flinders, A. J., Canniffe, D. P., . . . Hunter, C. N. (2019). Engineering of B800 bacteriochlorophyll binding site specificity in the <i>Rhodobacter sphaeroides</i> LH2 antenna. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1860(3), 209-223. doi:10.1016/j.bbabio.2018.11.008
Characterization of chlorophyll f synthase heterologously produced in Synechococcus sp. PCC 7002.
Shen, G., Canniffe, D. P., Ho, M. -Y., Kurashov, V., van der Est, A., Golbeck, J. H., & Bryant, D. A. (2019). Characterization of chlorophyll f synthase heterologously produced in Synechococcus sp. PCC 7002.. Photosynthesis research. doi:10.1007/s11120-018-00610-9
Biosynthesis of chlorophylls and bacteriochlorophylls in green bacteria
Thweatt, J. L., Canniffe, D. P., & Bryant, D. A. (2019). Biosynthesis of chlorophylls and bacteriochlorophylls in green bacteria. In METABOLISM, STRUCTURE AND FUNCTION OF PLANT TETRAPYRROLES: INTRODUCTION, MICROBIAL AND EUKARYOTIC CHLOROPHYLL SYNTHESIS AND CATABOLISM (Vol. 90, pp. 35-89). doi:10.1016/bs.abr.2019.03.002
Chapter Two Biosynthesis of chlorophylls and bacteriochlorophylls in green bacteria
Thweatt, J. L., Canniffe, D. P., & Bryant, D. A. (2019). Chapter Two Biosynthesis of chlorophylls and bacteriochlorophylls in green bacteria. In Metabolism, Structure and Function of Plant Tetrapyrroles: Introduction, Microbial and Eukaryotic Chlorophyll Synthesis and Catabolism (Vol. 90, pp. 35-89). Elsevier. doi:10.1016/bs.abr.2019.03.002
2018
A paralog of a bacteriochlorophyll biosynthesis enzyme catalyzes the formation of 1,2-dihydrocarotenoids in green sulfur bacteria
Canniffe, D. P., Thweatt, J. L., Chew, A. G. M., Hunter, C. N., & Bryant, D. A. (2018). A paralog of a bacteriochlorophyll biosynthesis enzyme catalyzes the formation of 1,2-dihydrocarotenoids in green sulfur bacteria. JOURNAL OF BIOLOGICAL CHEMISTRY, 293(39), 15233-15242. doi:10.1074/jbc.RA118.004672
<SUP>15</SUP>N photo-CIDNP MAS NMR analysis of reaction centers of <i>Chloracidobacterium thermophilum</i>
Zill, J. C., He, Z., Tank, M., Ferlez, B. H., Canniffe, D. P., Lahav, Y., . . . Matysik, J. (2018). <SUP>15</SUP>N photo-CIDNP MAS NMR analysis of reaction centers of <i>Chloracidobacterium thermophilum</i>. PHOTOSYNTHESIS RESEARCH, 137(2), 295-305. doi:10.1007/s11120-018-0504-1
Engineered biosynthesis of bacteriochlorophyll g(F) in Rhodobacter sphaeroides
Ortega-Ramos, M., Canniffe, D. P., Radle, M. I., Hunter, C. N., Bryant, D. A., & Golbeck, J. H. (2018). Engineered biosynthesis of bacteriochlorophyll g<sub>F</sub> in <i>Rhodobacter</i> <i>sphaeroides</i>. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1859(7), 501-509. doi:10.1016/j.bbabio.2018.02.006
Cryo-EM structure of the Blastochloris viridis LH1-RC complex at 2.9 angstrom
Qian, P., Siebert, C. A., Wang, P., Canniffe, D. P., & Hunter, C. N. (2018). Cryo-EM structure of the Blastochloris viridis LH1-RC complex at 2.9 angstrom. NATURE, 556, 203-208. doi:10.1038/s41586-018-0014-5
How nature designs light-harvesting antenna systems: design principles and functional realization in chlorophototrophic prokaryotes
Bryant, D. A., & Canniffe, D. P. (2018). How nature designs light-harvesting antenna systems: design principles and functional realization in chlorophototrophic prokaryotes. JOURNAL OF PHYSICS B-ATOMIC MOLECULAR AND OPTICAL PHYSICS, 51(3). doi:10.1088/1361-6455/aa9c3c
Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex
Jackson, P. J., Hitchcock, A., Swainsbury, D. J. K., Qian, P., Martin, E. C., Farmer, D. A., . . . Hunter, C. N. (2018). Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1859(2), 119-128. doi:10.1016/j.bbabio.2017.11.001
Complete enzyme set for chlorophyll biosynthesis in Escherichia coli
Chen, G. E., Canniffe, D. P., Barnett, S. F. H., Hollingshead, S., Brindley, A. A., Vasilev, C., . . . Hunter, C. N. (2018). Complete enzyme set for chlorophyll biosynthesis in Escherichia coli. SCIENCE ADVANCES, 4(1). doi:10.1126/sciadv.aaq1407
2017
Three classes of oxygen-dependent cyclase involved in chlorophyll and bacteriochlorophyll biosynthesis
Chen, G. E., Canniffe, D. P., & Hunter, C. N. (2017). Three classes of oxygen-dependent cyclase involved in chlorophyll and bacteriochlorophyll biosynthesis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114(24), 6280-6285. doi:10.1073/pnas.1701687114
Light regulation of pigment and photosystem biosynthesis in cyanobacteria
Ho, M. -Y., Soulier, N. T., Canniffe, D. P., Shen, G., & Bryant, D. A. (2017). Light regulation of pigment and photosystem biosynthesis in cyanobacteria. CURRENT OPINION IN PLANT BIOLOGY, 37, 24-33. doi:10.1016/j.pbi.2017.03.006
2016
Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll-Protein Complex
Hitchcock, A., Jackson, P. J., Chidgey, J. W., Dickman, M. J., Hunter, C. N., & Canniffe, D. P. (2016). Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll-Protein Complex. ACS Synthetic Biology, 5(9), 948-954. doi:10.1021/acssynbio.6b00069
Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of photosystem II
Ho, M. -Y., Shen, G., Canniffe, D. P., Zhao, C., & Bryant, D. A. (2016). Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of photosystem II. SCIENCE, 353(6302). doi:10.1126/science.aaf9178
Absence of the <i>cbb</i><sub>3</sub> Terminal Oxidase Reveals an Active Oxygen-Dependent Cyclase Involved in Bacteriochlorophyll Biosynthesis in <i>Rhodobacter sphaeroides</i>
Chen, G. E., Canniffe, D. P., Martin, E. C., & Hunter, C. N. (2016). Absence of the <i>cbb</i><sub>3</sub> Terminal Oxidase Reveals an Active Oxygen-Dependent Cyclase Involved in Bacteriochlorophyll Biosynthesis in <i>Rhodobacter sphaeroides</i>. JOURNAL OF BACTERIOLOGY, 198(15), 2056-2063. doi:10.1128/JB.00121-16
Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in <i>Acaryochloris marina</i>
Chen, G. E., Hitchcock, A., Jackson, P. J., Chaudhuri, R. R., Dickman, M. J., Hunter, C. N., & Canniffe, D. P. (2016). Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in <i>Acaryochloris marina</i>. JOURNAL OF BACTERIOLOGY, 198(9), 1393-1400. doi:10.1128/JB.00925-15
2014
Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis
Canniffe, D. P., Chidgey, J. W., & Hunter, C. N. (2014). Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis. BIOCHEMICAL JOURNAL, 462, 433-440. doi:10.1042/BJ20140163
A Cyanobacterial Chlorophyll Synthase-HliD Complex Associates with the Ycf39 Protein and the YidC/Alb3 Insertase
Chidgey, J. W., Linhartova, M., Komenda, J., Jackson, P. J., Dickman, M. J., Canniffe, D. P., . . . Sobotka, R. (2014). A Cyanobacterial Chlorophyll Synthase-HliD Complex Associates with the Ycf39 Protein and the YidC/Alb3 Insertase. PLANT CELL, 26(3), 1267-1279. doi:10.1105/tpc.114.124495
Engineered biosynthesis of bacteriochlorophyll b in Rhodobacter sphaeroides
Canniffe, D. P., & Hunter, C. N. (2014). Engineered biosynthesis of bacteriochlorophyll b in Rhodobacter sphaeroides. BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - Bioenergetics, 1837(10), 1611-1616. doi:10.1016/j.bbabio.2014.07.011
2013
Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in <i>Rhodobacter sphaeroides</i> and evidence for the existence of a third distinct class of the enzyme
Canniffe, D. P., Jackson, P. J., Hollingshead, S., Dickman, M. J., & Hunter, C. N. (2013). Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in <i>Rhodobacter sphaeroides</i> and evidence for the existence of a third distinct class of the enzyme. BIOCHEMICAL JOURNAL, 450, 397-405. doi:10.1042/BJ20121723
2012
Conserved Chloroplast Open-reading Frame <i>ycf54</i> Is Required for Activity of the Magnesium Protoporphyrin Monomethylester Oxidative Cyclase in <i>Synechocystis</i> PCC 6803
Hollingshead, S., Kopecna, J., Jackson, P. J., Canniffe, D. P., Davison, P. A., Dickman, M. J., . . . Hunter, C. N. (2012). Conserved Chloroplast Open-reading Frame <i>ycf54</i> Is Required for Activity of the Magnesium Protoporphyrin Monomethylester Oxidative Cyclase in <i>Synechocystis</i> PCC 6803. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(33), 27823-27833. doi:10.1074/jbc.M112.352526
Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities
Li, M., Canniffe, D. P., Jackson, P. J., Davison, P. A., FitzGerald, S., Dickman, M. J., . . . Huang, W. E. (2012). Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities. ISME JOURNAL, 6(4), 875-885. doi:10.1038/ismej.2011.150
Highly confined surface imaging by solid immersion total internal reflection fluorescence microscopy
Wang, L., Vasilev, C., Canniffe, D. P., Wilson, L. R., Hunter, C. N., & Cadby, A. J. (2012). Highly confined surface imaging by solid immersion total internal reflection fluorescence microscopy. OPTICS EXPRESS, 20(3), 3311-3324. doi:10.1364/OE.20.003311