Return to Case Study 3 - Demonstration of micropropagation
Media to demonstrate development of tissue culture in the African Violet
| MS salts g.l-1 |
NAA mg.l-1 |
BAP mg.l-1 |
sucrose % |
|
| 1. Control | 4.71 |
0.5 |
0.5 |
3.0 |
| 2. vary sucrose - 0 | as control | as control | as control | 0.0 |
| 3. vary sucrose -1% | as control | as control | as control | 1.0 |
| 4. vary sucrose - 5% | as control | as control | as control | 5.0 |
| 5. vary BAP - 0 | as control | as control | 0.0 |
as control |
| 6. vary BAP - 1/5th | as control | as control | 0.1 |
as control |
| 7. vary BAP - x10 | as control | as control | 5.0 |
as control |
| 8. vary NAA - 0 | as control | 0 |
as control | as control |
| 9. vary NAA - 1/5th | as control | 0.1 |
as control | as control |
| 10. vary NAA - x10 | as control | 5.0 |
as control | as control |
| 11. vary MS - 0 | 0 |
as control | as control | as control |
| 12. vary MS - 1/10th | 0.47 |
as control | as control | as control |
| 13. vary MS - x2 | 9.52 |
as control | as control | as control |
Key to abbreviations:
MS salts - Murashige and Skoog inorganic medium
NAA - naphthalene acetic acid (auxin)
BAP - benzylamine purine (cytokinin)
Murashige and Skoog (MS) is one of the most commonly used basic media in plant tissue culture work. It is regarded as a 'high salt' medium because of its macroelement composition.
Since accurate and reproducible preparation of the growth medium is essential, it is now usually obtained from a commercial source in powdered or liquid concentrate. Growth regulators are purchased similarly.
All media are solidified with 10 g.l-1 agar, and sterilised by autoclaving, then dispensed into 9cm petri dishes