Cloning vectors
pGEM-T our favourite- use this for sequencing
psl1180 phagemid shuttle vector with lots of restriction sites
Bacterial vectors
pGEX GST gene fusion vectors (4T-1,2,3 & 2T)
pGAT2 6xHis,GST plus thrombin site
pTrcHis (A,B,C Invitrogen) 6xHis fusion vectors
pET-15b (Novagen) 6xHis fusion vector plus thrombin site
pET-44a N-terminal Nus tag, N and C-terminal 6xHis tags
Mammalian expression vectors
pcDNA3.1+ CMV driven expression vector (Invitrogen)
pcDNA3.1/zeo+
HA-pcDNA3.1/zeo+ N-teminal HA tag followed by EcoR1 site.
pcB6
pTRE-HA HA epitope fusion vectorwith Tet responsive element (Clontech)
pTK-Hyg hyromycin resistance vector
pDsRED1-N1 C-terminal RFP
pCMV-Tag2 (A,B,C) N-termina FLAG tag
pEGFP-C1 (C1,C2,C3) N-terminal EGFP tag (Clontech)
pEGFP-N1 (N1,N2,N3) C-terminal EGFP tag (Clontech)
pECFP-C1 cyan
pEYFP-C1 yellow
pEYFP-N1 yellow
pEBFP-N1 blue
photoactivatable GFP-C1 and N1 (covered by MTA)
Yeast expression vectors
pUG36 N-terminal GFP tag
Baculovirus vectors
pVL1392 and pVL1393 (Pharmingen)
pAcHLT-A N-terminal 6xHis tag, thrombin site
pAcGHLT-A N-terminal GST, 6xHis tags thrombin site
pAcG2T N-terminal GST tag thrombin site