from Cleary's lab
cDNA sequence:
gctggctgggaagatggcggcgggaacctgggccgccgccgccgccgccg
ccgccgccgcggagcgaaccaggggtgtccggggtgcgcggtccagggcc
ggggccgggccatgagcgcgccgtcctcgagtccccgagccgcggagccc
gcccgcgcccctcgggccgccccgcgtccctcgccatggcgcggctcgcg
gactacttcgtgctggtggcgttcgggccgcacccgcgcgggagtgggga
aggccagggccagattctgcagcgcttcccagagaaggactgggaggaca
acccattcccccagggcatcgagctgttttgccagcccagcgggtggcag
ctgtgtcccgagaggaatccaccgaccttctttgttgctgtcctcaccga
catcaactccgagcgccactactgcgcctgcttgaccttctgggagccag
cggagccttcacaggaaacgacgcgcgtggaggatgccacagagagggag
gaagagggggatgagggaggccagacccacctgtctcccacagcacctgc
cccatctgcccagctgtttgcaccgaagacgctggtactggtgtcgcgac
tcgaccacacggaggtgttcaggaacagccttggcctcatctatgccatc
cacgtggagggcctgaatgtgtgcctggagaacgtgattgggaacctgct
gacgtgcactgtgcccctggctgggggctcgcagaggacgatctctttgg
gggctggtgaccggcaggtcatccagactccactggccgactcgctgccc
gtcagccgctgcagcgtggccctgctcttccgccagctaggcatcaccaa
cgtgctgtctttgttctgtgccgccctcacggagcacaaggttctcttcc
tgtcccggagctaccagcggctcgccgatgcctgtaggggcctcctggca
ctgctgtttcctctcagatacagcttcacctatgtgcccatcctgccggc
tcagctgctggaggtcctcagcacacccacgcccttcatcattggggtca
acgcggccttccaggcagagacccaggagctgctcgatgtgattgttgct
gatctggatggagggacggtcaccattcctgagtgtgtgcacattccacc
cttgccagagccactgcagagtcagacgcacagtgtgctgagcatggtcc
tggacccggagctggagttggctgacctcgccttccctccgcccacgaca
tccacctcctccctgaagatgcaggacaaggagctgcgcgcggtcttcct
gcggctgttcgctcagctgctgcagggctatcgctggtgcctgcacgtcg
tgcgcatccacccggagcctgtcatccgcttccataaggcagccttcctg
ggccagcgtgggctggtagaggacgatttcctgatgaaggtgctggaggg
catggcctttgctggctttgtgtcagagcgtggggtcccataccgcccta
cggacctgttcgatgagctggtggcccacgaggtggcaaggatgcgggcg
gatgagaaccacccccagcgtgtcctgcgtcacgtccaggaactggcaga
gcagctctacaagaacgagaacccgtacccagccgtggcgatgcacaagg
tacagaggcccggtgagagcagccacctgcgacgggtgccccgacccttc
ccccggctggatgagggcaccgtgcagtggatcgtggaccaggctgcagc
caagatgcagggtgcacccccagctgtgaaggccgagaggaggaccaccg
tgccctcagggccccccatgactgccatactggagcggtgcagtgggctg
catgtcaacagcgcccggcggctggaggttgtgcgcaactgcatctccta
cgtgtttgaggggaaaatgcttgaggccaagaagctgctcccagccgtgt
tgagggccctgaaggggcgagctgcccgccgctgcctcgcccaggagctg
cacctgcatgtgcagcagaaccgtgcggtcctggaccaccagcagtttga
ctttgtcgtccgtatgatgaactgctgcctgcaggactgcacttctctgg
acgagcatggcattgcggcggctctgctgcctctggtcacagccttctgc
cggaagctgagcccgggggtgacgcagtttgcatacagctgtgtgcagga
gcacgtggtgtggagcacgccacagttctgggaggccatgttctatgggg
atgtgcagactcacatccgggccctctacctggagcccacggaggacctg
gcccccgcccaggaggttggggaggcaccttcccaggaggacgagcgctc
tgccctagacgtggcttctgagcagcggcgcttgtggccaactctgagtc
gtgagaagcagcaggagctggtgcagaaggaggagagcacggtgttcagc
caggccatccactatgccaaccgcatgagctacctcctcctgcccctgga
cagcagcaagagccgcctacttcgggagcgtgccgggctgggcgacctgg
agagcgccagcaacagcctggtcaccaacagcatggctggcagtgtggcc
gagagctatgacacggagagcggcttcgaggatgcagagacctgcgacgt
agctggggctgtggtccgcttcatcaaccgctttgtggacaaggtctgca
cggagagtggggtcaccagcgaccacctcaaggggctgcatgtcatggtg
ccagacattgtccagatgcacatcgagaccctggaggccgtgcagcggga
gagccggaggctgccgcccatccagaagcccaagctgctgcggccgcgcc
tgctgccgggtgaggagtgtgtgctggacggcctgcgcgtctacctgctg
ccggatgggcgtgaggagggcgcggggggcagtgctgggggaccagcatt
gctcccagctgagggcgccgtcttcctcaccacgtaccgggtcatcttca
cggggatgcccacggaccccctggttggggagcaggtggtggtccgctcc
ttcccggtggctgcgctgaccaaggagaagcgcatcagcgtccagacccc
tgtggaccagctcctgcaggacgggctccagctgcgctcctgcacattcc
agctgctgaaaatggcctttgacgaggaggtggggtctgacagcgccgag
ctcttccgtaagcagctgcataagctgcggtacccgccggacatcagggc
cacctttgcgttcaccttgggctctgcccacacacctggccggccaccgc
gagtcaccaaggacaagggtccttccctcagaaccctgtcccggaacctg
gtcaagaacgccaagaagaccatcgggcggcagcatgtcactcgcaagaa
gtacaacccccccagctgggagcaccggggccagccgccccctgaggacc
aggaggacgagatctcagtgtcggaggagctggagcccagcacgctgacc
ccgtcctcagccctgaagccctccgaccgcatgaccatgagcagcctggt
ggaaagggcttgctgtcgcgactaccagcgcctcggtctgggcaccctga
gcagcagcctgagccgggccaagtctgagcccttccgcatttctccggtc
aaccgcatgtatgccatctgccgcagctacccagggctgctgatcgtgcc
ccagagtgtccaggacaacgccctgcagcgcgtgtcccgctgctaccgcc
agaaccgcttccccgtggtctgctggcgcagcgggcggtccaaggcggtg
ctgctgcgctctggaggcctgcatggcaaaggtgtcgtcggcctcttcaa
ggcccagaacgcaccttctccaggccagtcccaggcggactcgagtagcc
tggagcaggagaagtacctgcaggctgtggtcagctccatgccccgctac
gccgacgcgtcgggacgcaacacgcttagcggcttctcctcagcccacat
gggcagtcacggtaagtggggcagtgtccggaccagtggacgcagcagtg
gccttggcaccgatgtgggctcccggctagctggcagagacgcgctggcc
ccaccccaggccaacgggggccctcccgacccgggcttcctgcgtccgca
gcgagcagccctctatatccttggggacaaagcccagctcaagggtgtgc
ggtcagaccccctgcagcagtgggagctggtgcccattgaggtattcgag
gcacggcaggtgaaggctagcttcaagaagctgctgaaagcatgtgtccc
aggctgccccgctgctgagcccagcccagcctccttcctgcgctcactgg
aggactcagagtggctgatccagatccacaagctgctgcaggtgtctgtg
ctggtggtggagctcctggattcaggctcctccgtgctggtgggcctgga
ggatggctgggacatcaccacccaggtggtatccttggtgcagctgctct
cagaccccttctaccgcacgctggagggctttcgcctgctggtggagaag
gagtggctgtccttcggccatcgcttcagccaccgtggagctcacaccct
ggccgggcagagcagcggcttcacacccgtcttcctgcagttcctggact
gcgtacaccaggtccacctgcagttccccatggagtttgagttcagccag
ttctacctcaagttcctcggctaccaccatgtgtcccgccgtttccggac
cttcctgctcgactctgactatgagcgcattgagctggggctgctgtatg
aggagaagggggaacgcaggggccaggtgccgtgcaggtctgtgtgggag
tatgtggaccggctgagcaagaggacgcctgtgttccacaattacatgta
tgcgcccgaggacgcagaggtcctgcggccctacagcaacgtgtccaacc
tgaaggtgtgggacttctacactgaggagacgctggccgagggccctccc
tatgactgggaactggcccaggggccccctgaacccccagaggaagaacg
gtctgatggaggcgctccccagagcaggcgccgcgtggtgtggccctgtt
acgacagctgcccgcgggcccagcctgacgccatctcacgcctgctggag
gagctgcagaggctggagacagagttgggccaacccgctgagcgctggaa
ggacacctgggaccgggtgaaggctgcacagcgcctcgagggccggccag
acggccgtggcacccctagctccctccttgtgtccaccgcaccccaccac
cgtcgctcgctgggtgtgtacctgcaggaggggcccgtgggctccaccct
gagcctcagcctggacagcgaccagagtagtggctcaaccacatccggct
cccgtcaggctgcccgccgcagcaccagcaccctgtacagccagttccag
acagcagagagtgagaacaggtcctacgagggcactctgtacaagaaggg
ggccttcatgaagccttggaaggcccgctggttcgtgctggacaagacca
agcaccagctgcgctactacgaccaccgtgtggacacagagtgcaagggt
gtcatcgacttggcggaggtggaggctgtggcacctggcacgcccactat
gggtgcccctaagactgtggacgagaaggccttctttgacgtgaagacaa
cgcgtcgcgtttacaacttctgtgcccaggacgtgccctcggcccagcag
tgggtggaccggatccagagctgcctgtcggacgcctgagcctcccagcc
ctgcccggctgctctgcttccggtcgttaccgaccactaggggtgggcag
ggccgccccggccatgtttacagccccggccctcgacagtattgaggccc
cgagcccccagcacttgtgtgtacagcccccgtccccgccccgccccgcc
cggccggccctaacttattttggcgtcacagctgagcaccgtgccgggag
gtggccaaggtacagcccgcaatgggcctgtaaatagtccggccccgtca
gcgtgtgctggtccagccagcggctgcaggcgagtttctagaaccagagt
ctatataaagagagaactaacgccaaaaaaaaaa
Protein Sequence (1868 aa):
Note: Original MTMR5 from Cleary's lab had already an aa mutated (Ala1325 to Val)
MARLADYFVLVAFGPHPRGSGEGQGQILQRFPEKDWEDNPFPQGIELFCQ
PSGWQLCPERNPPTFFVAVLTDINSERHYCACLTFWEPAEPSQETTRVED
ATEREEEGDEGGQTHLSPTAPAPSAQLFAPKTLVLVSRLDHTEVFRNSLG
LIYAIHVEGLNVCLENVIGNLLTCTVPLAGGSQRTISLGAGDRQVIQTPL
ADSLPVSRCSVALLFRQLGITNVLSLFCAALTEHKVLFLSRSYQRLADAC
RGLLALLFPLRYSFTYVPILPAQLLEVLSTPTPFIIGVNAAFQAETQELL
DVIVADLDGGTVTIPECVHIPPLPEPLQSQTHSVLSMVLDPELELADLAF
PPPTTSTSSLKMQDKELRAVFLRLFAQLLQGYRWCLHVVRIHPEPVIRFH
KAAFLGQRGLVEDDFLMKVLEGMAFAGFVSERGVPYRPTDLFDELVAHEV
ARMRADENHPQRVLRHVQELAEQLYKNENPYPAVAMHKVQRPGESSHLRR
VPRPFPRLDEGTVQWIVDQAAAKMQGAPPAVKAERRTTVPSGPPMTAILE
RCSGLHVNSARRLEVVRNCISYVFEGKMLEAKKLLPAVLRALKGRAARRC
LAQELHLHVQQNRAVLDHQQFDFVVRMMNCCLQDCTSLDEHGIAAALLPL
VTAFCRKLSPGVTQFAYSCVQEHVVWSTPQFWEAMFYGDVQTHIRALYLE
PTEDLAPAQEVGEAPSQEDERSALDVASEQRRLWPTLSREKQQELVQKEE
STVFSQAIHYANRMSYLLLPLDSSKSRLLRERAGLGDLESASNSLVTNSM
AGSVAESYDTESGFEDAETCDVAGAVVRFINRFVDKVCTESGVTSDHLKG
LHVMVPDIVQMHIETLEAVQRESRRLPPIQKPKLLRPRLLPGEECVLDGL
RVYLLPDGREEGAGGSAGGPALLPAEGAVFLTTYRVIFTGMPTDPLVGEQ
VVVRSFPVAALTKEKRISVQTPVDQLLQDGLQLRSCTFQLLKMAFDEEVG
SDSAELFRKQLHKLRYPPDIRATFAFTLGSAHTPGRPPRVTKDKGPSLRT
LSRNLVKNAKKTIGRQHVTRKKYNPPSWEHRGQPPPEDQEDEISVSEELE
PSTLTPSSALKPSDRMTMSSLVERACCRDYQRLGLGTLSSSLSRAKSEPF
RISPVNRMYAICRSYPGLLIVPQSVQDNALQRVSRCYRQNRFPVVCWRSG
RSKAVLLRSGGLHGKGVVGLFKAQNAPSPGQSQADSSSLEQEKYLQAVVS
SMPRYADASGRNTLSGFSSAHMGSHGKWGSVRTSGRSSGLGTDVGSRLAG
RDALAPPQANGGPPDPGFLRPQRAALYILGDKAQLKGVRSDPLQQWELVP
IEVFEARQVKASFKKLLKACVPGCPAAEPSPASFLRSLEDSEWLIQIHKL
LQVSVLVVELLDSGSSVLVGLEDGWDITTQVVSLVQLLSDPFYRTLEGFR
LLVEKEWLSFGHRFSHRGAHTLAGQSSGFTPVFLQFLDCVHQVHLQFPME
FEFSQFYLKFLGYHHVSRRFRTFLLDSDYERIELGLLYEEKGERRGQVPC
RSVWEYVDRLSKRTPVFHNYMYAPEDAEVLRPYSNVSNLKVWDFYTEETL
AEGPPYDWELAQGPPEPPEEERSDGGAPQSRRRVVWPCYDSCPRAQPDAI
SRLLEELQRLETELGQPAERWKDTWDRVKAAQRLEGRPDGRGTPSSLLVS
TAPHHRRSLGVYLQEGPVGSTLSLSLDSDQSSGSTTSGSRQAARRSTSTL
YSQFQTAESENRSYEGTLYKKGAFMKPWKARWFVLDKTKHQLRYYDHRVD
TECKGVIDLAEVEAVAPGTPTMGAPKTVDEKAFFDVKTTRRVYNFCAQDV
PSAQQWVDRIQSCLSDA
SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF