The open reading frame (ORF) for human MTMR3 was amplified by polymerase chain reaction (PCR) from pBluescipt-KIAA0371 (cDNA, a kind gift of T. Nagase, Japan), cloned into the pGEMT vector, and sequenced
cDNA sequence:
gtgagaaggcggcggcggcggcggagtagcagccggacgagacgtcccag
cggctcaggcgctgcccagcgccggccctggcagggagcctggtggggtg
gcggagggggagactgtgccgtggagggcctcgccatgtcctgctgcccg
acttccttgtgaaacctcctagtagaaaaagttcttgggacaaacttcat
ttgacttcaccataagggaaagaagagagccttgttggacactgaagaag
ggacggggatttctcctcctaatctgggcctcttgtcatggatgaagaga
ctcggcacagccttgagtgcatccaggccaatcagatctttcccaggaag
cagctgatccgggaggatgagaatcttcaggttcctttccttgaacttca
tggagagagcacagagtttgtgggccgtgccgaggatgccatcattgccc
tttccaattacagacttcacatcaagttcaaggagtctcttgttaatgtt
ccattacagcttatagaaagtgttgaatgccgagatatatttcagcttca
tttgacttgcaaagactgcaaagttatcaggtgtcagttttcaacctttg
agcagtgtcaagagtggctgaagagactgaacaacgcaatccgaccacct
gctaaaatagaagatctcttctcatttgcataccatgcttggtgcatgga
ggtctatgccagtgaaaaagagcaacatggagacctgtgcagaccagggg
agcatgtaacttcaaggtttaaaaacgaggtggagaggatgggttttgat
atgaacaacgcctggaggatttccaacatcaatgagaagtacaaattatg
tggtagctatcctcaagagctcatagtgcctgcctggatcactgacaaag
aactggaaagtgtatcaagtttcaggtcctggaagcgcatccctgccgtc
atctacaggcaccagagcaatggagctgtcattgcccgctgtggacagcc
agaggttagctggtggggctggcgaaatgcagatgatgagcatctggtac
agtcagtagccaaagcttgtgcctctgactcccgatcgagtggcagcaag
ctgtcaactaggaacacttctcgagactttcccaatgggggagacctttc
tgacgtggagttcgattcttctctgtcaaatgcttcaggagcagagagtt
tagccatccaaccgcagaagcttttgatcttggatgcacgctcctatgca
gctgctgtggcaaaccgagccaaaggaggaggctgcgaatgcccagagta
ttacccaaactgtgaagttgtgtttatggggatggcaaacattcattcta
ttcggaggagttttcagtctctgcggttgctgtgcactcagatgccagat
ccgggaaattggctatcagctcttgaaagcacaaaatggctccatcactt
gtctgtgcttctgaaatcagcgcttctggtagtgcatgctgtggatcagg
atcagcggccggtgctagtacactgctcagatggctgggaccgcaccccc
cagattgtggcattggctaagctcttgctggacccttattaccgaaccat
agagggtttccaggtcctcgtggaaatggagtggctggattttggccata
aatttgctgaccggtgtggtcatggggagaactcggatgatctgaatgaa
cgttgcccagtgtttctgcagtggcttgactgtgttcatcagcttcagag
gcaatttccttgctcttttgagttcaatgaagcattccttgtgaaactgg
tgcagcatacctattcctgcctgtttggaacattcctgtgcaacaacgcc
aaggagagaggggaaaagcatactcaggaacggacatgttccgtgtggtc
acttcttcgggcaggcaacaaggctttcaaaaacctactgtattcctctc
agtcagaagccgtgctgtaccctgtgtgccatgtgcgtaacctgatgctg
tggagtgcagtgtacctgccctgcccatccccaaccacccctgtggacga
cagctgtgcaccatacccagccccaggcaccagccctgatgatccccccc
tgagccggctaccaaagactagatcatacgacaatctgaccacagcctgt
gacaacacagtgcctctggccagccggcgctgcagcgaccccagcctgaa
cgagaagtggcaggagcaccggcgctcactagagctgagcagcctggctg
gccctggagaggatcccctttctgccgacagcctagggaagcccaccaga
gtgccggggggtgccgagctttctgttgcagccggagtagctgaggggca
gatggagaacatcttgcaggaggccaccaaagaggagagtggagtagagg
aacctgcccacagggcaggcattgagatacaggagggtaaagaggaccct
ctcttagaaaaggagagcaggaggaagacacctgaggcctcagccattgg
acttcaccaagacccagaactgggtgatgctgctctgaggagccatctgg
atatgagctggcctctgttctcacagggcatttctgaacagcagagtggg
ctcagtgttctcctcagttctctccaggtcccccccaggggagaggattc
cctggaggtccctgtggagcagtttcgaatagaagagattgcagagggta
gggaggaagcagttcttccaatcccagtagatgcaaaagttggctatggt
acctcacagtcatgttctctgctaccttcccaagtcccttttgagaccag
aggaccaaacgtggacagttctacagacatgttagtggaagataaggtga
agtcagtaagtgggccccaaggtcatcatagatcttgccttgtaaatagt
ggcaaggacaggcttcctcagaccatggaacccagcccttcagagacaag
cctggtcgagaggccccaagtggggtctgtggtgcataggacttcccttg
gcagcactctcagcctgacacgttccccttgtgccttgcctttagccgaa
tgtaaagaggggcttgtgtgcaatggtgccccagagactgaaaacagggc
ctcagagcagcccccaggtcttagcaccctccagatgtaccccacaccca
atgggcattgcgccaatggggaggctggtaggagcaaggactcactgagc
cgtcagctgtctgctatgagctgcagctctgcccacttacactcaaggaa
cttgcaccacaagtggctgcatagccactcaggaaggccatctgcaacca
gcagccccgaccagccttcccgcagccacctggacgatgatggcatgtca
gtgtacacagacacgatccaacagcgcctgcgtcagattgagtcaggcca
ccagcaggaagtagaaactttgaagaaacaagtccaggagctgaagagtc
gcctggagagccagtacctgaccagctccctacactttaatggagacttt
ggggatgaggtgatgacccgttggcttcctgaccacctggccgcccactg
ctatgcgtgcgacagtgccttctggcttgccagcaggaagcaccactgca
gggacactgaccgtgttgatcaaacgtggaattgtgggaacgtattctgc
tccagttgttgtaaccagaaggttccagttcccagccagcagctctttga
acccagtcgagtatgcaagtcttgctatagcagcctacatcccacaagct
ccagcattgaccttgaactggataagcccattgctgccacttccaactga
agctcagtgacctgggtgggcagtggccaagctgctgttcctatgacagg
cccactcaacctgggcagaccgagaggcccgtgcactttggaatgggagc
gtggaaccacctgtacagagtgacagatttgggatgcaccactggattgt
agattgatttttctttcctgtccccctactccctccctaccttttccatc
ctcctcctctgccttcaaaaaaggaaactttcccttggttgtcttaattt
tttttttttttgatggaagaccaagggttgccaggcccactgtaactgcc
gagctgcctgctgtcacgtgacactgagggatggcttgtttcttccgggt
gggaggatggtggtcagagccaggagtatggagatctgagaccgtgagca
gggaagaaagccagtgctaacatgcaaccattcctcacgccaccgccaca
tcagagctggttgggaaccctttgctgctggggaggctggaagcatattc
cccaagagcactgccctgggcatcatctccctcctgcgaggagctgagcc
agtcccctcacagatggataaatgaggctgatgtttggagggagaggcac
acggtaaatggcatccccttaggcgcctctttgggaaaggaaagtggatg
ctcctttgaggcaggcgaggggctgggagtgggtagccgtcatgttgtcc
cccgtgggatcccatttttaacttgacccagattgtcttgggctcctttt
actttcagggggctgctttcctgggccaggctgtgtagcacttcccaccc
tcaggcatgagtacagactggtcaaaatgtttatgcagtcaaggccaaag
cctgccctgagcccagacacacctgggtccccattctggggcctggtccc
ctaaacaatctcttcctcagccagcacagggaaatccagactcagggttc
cagaaatcccccgttcccaaaccaaaccaatcatggatcttccctttaag
gggtagaatcagcctttagagttacaagcccctggaaaagggagcaggtc
ccatacaatcagcctttctcctcttctcttcttgggacaggaataactgc
tgagcagtccccctttgccactcctggctgtctgagtgggcacttgtctg
gctgctggccagcagtgaagggccacttggtgacttcgtagggttcctta
gagaagtgacatggccttgaggagattcagggcacctttcctcagtgagc
tttgatatggtccaaaaacagccttaaatcaagaatatttgtggaggtag
gtgtggggaaggttggagaagagttaggtttgtgctttgtaatcttggca
tccatgttttgtgcctgccctccctcttggggaggccatgcttgactctg
ctgaaagctgctaactggtgacagggtggatcatggtgaggactaggggt
aaggtcagggaatgcttaagctctggccctgccctgtattcctcttcccc
ttggtagcagttccctgaccaagggcaaggtgtgtctcaggaaggtggtt
ctgtgcatgcctgggtgtggttaaggcgtggcccagaaggcttccctggt
gctctcagtccaggcaaagcccagagcatctgagcacgtctctgacttcc
agtggcaacacagtttgaacgtggtgaaacagggtgtagttcttttccac
attctgtgtcatctagtcgtgcaggtagggaaaaagttggtctaattgaa
gattgtgcatttcctagtgacaggtgccaagaggttatgatacgggtttc
ttgggtctgatgtacagtgtgaataaatgcttgcagctcttagctctttt
ttgatcgatgaagcacttttttattaatattttcctttgttaaaggagga
accgtaactctccatagctgtacatataacccttttctcctaaagaggag
tcagtcagtgctcctatatttttcattttttgtcaaagcaagaagtaaat
actttagaattgttaaatatataaataaagcaaataaagttgagtgttcc
acatggtagcatttgctaacacttcctaa
Protein Sequence (1198 aa):
MDEETRHSLECIQANQIFPRKQLIREDENLQVPFLELHGESTEFVGRAED
AIIALSNYRLHIKFKESLVNVPLQLIESVECRDIFQLHLTCKDCKVIRCQ
FSTFEQCQEWLKRLNNAIRPPAKIEDLFSFAYHAWCMEVYASEKEQHGDL
CRPGEHVTSRFKNEVERMGFDMNNAWRISNINEKYKLCGSYPQELIVPAW
ITDKELESVSSFRSWKRIPAVIYRHQSNGAVIARCGQPEVSWWGWRNADD
EHLVQSVAKACASDSRSSGSKLSTRNTSRDFPNGGDLSDVEFDSSLSNAS
GAESLAIQPQKLLILDARSYAAAVANRAKGGGCECPEYYPNCEVVFMGMA
NIHSIRRSFQSLRLLCTQMPDPGNWLSALESTKWLHHLSVLLKSALLVVH
AVDQDQRPVLVHCSDGWDRTPQIVALAKLLLDPYYRTIEGFQVLVEMEWL
DFGHKFADRCGHGENSDDLNERCPVFLQWLDCVHQLQRQFPCSFEFNEAF
LVKLVQHTYSCLFGTFLCNNAKERGEKHTQERTCSVWSLLRAGNKAFKNL
LYSSQSEAVLYPVCHVRNLMLWSAVYLPCPSPTTPVDDSCAPYPAPGTSP
DDPPLSRLPKTRSYDNLTTACDNTVPLASRRCSDPSLNEKWQEHRRSLEL
SSLAGPGEDPLSADSLGKPTRVPGGAELSVAAGVAEGQMENILQEATKEE
SGVEEPAHRAGIEIQEGKEDPLLEKESRRKTPEASAIGLHQDPELGDAAL
RSHLDMSWPLFSQGISEQQSGLSVLLSSLQVPPRGEDSLEVPVEQFRIEE
IAEGREEAVLPIPVDAKVGYGTSQSCSLLPSQVPFETRGPNVDSSTDMLV
EDKVKSVSGPQGHHRSCLVNSGKDRLPQTMEPSPSETSLVERPQVGSVVH
RTSLGSTLSLTRSPCALPLAECKEGLVCNGAPETENRASEQPPGLSTLQM
YPTPNGHCANGEAGRSKDSLSRQLSAMSCSSAHLHSRNLHHKWLHSHSGR
PSATSSPDQPSRSHLDDDGMSVYTDTIQQRLRQIESGHQQEVETLKKQVQ
ELKSRLESQYLTSSLHFNGDFGDEVTSIPDSESNLDQNCLSRCSTEIFSE
ASWEQVDKQDTEMTRWLPDHLAAHCYACDSAFWLASRKHHCRNCGNVFCS
SCCNQKVPVPSQQLFEPSRVCKSCYSSLHPTSSSIDLELDKPIAATSN
SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF