BIOL401 - Macromolecular Structure and Function

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BIOL401 - Macromolecular Structure and Function

Module co-ordinator : Dr. J.S. Easterby

Location

Life Sciences LT1

Times

Monday, Tuesday + Wednesday (Wk 8-9): 09.00

Week
Lecture Content

1-2
3 Dr. B.R. Barraclough
Investigation of protein structure - molecular biological methods.

Growth of sequence data, cDNA, genomic sequences.

Analysis of unknown DNA sequence with particular reference to protein coding regions.

Prediction of protein structure and function..

3-4
4 Dr. G. Britton
Investigation of protein structure - physical methods

NMR, ESR, X-ray.

5-8
7 Dr. J.S. Easterby
Acquisition of protein structure - folding in vitro and in vivo.

Classical models of protein folding.

Folding in vivo; temporal and spatial organisation of events; protein disulphide isomerase and chaperonins.

Mechanisms of protein action - enzymic catalysis

Transition state - stabilisation and analogues; covalent and non-covalent catalysis.

Mechanism of chymotrypsin; zymogen activation; chemical, kinetic and crystallographic studies.

Mechanism of lysozyme; chemical, kinetic and crystallographic studies.

8-10
6 Dr. A.D. Bates
DNA-protein interactions

Survey of examples of the structure of DNA-protein complexes.

Parameters describing DNA conformation and application to DNA-protein complexes.

Higher-order DNA-protein structures.

DNA Topology

DNA topology - basics and importance in vivo.

DNA topoisomerases - mechanisms, drug interactions.

Roles of topoisomerases in vivo - replication, transcription, recombination, cell division.

10-11
3 Dr J.S. Easterby
Metabolic Control Analysis and Metabolic Pathway Dynamics

Introduction to basic concepts. Flux and concentration control coefficients. Flux and concentration summation properties. Connectivity.

Examples of control analysis. Genetic applications and the molecular basis of dominance. Biotechnological importance. Metabolite channelling.

Temporal response of pathways. Temporal control analysis.

CONTENTS | NEXT PAGE

Location	Life Sciences LT1
Times	Monday, Tuesday + Wednesday (Wk 8-9): 09.00

Week	Lecture	Content
1-2	3	Dr. B.R. Barraclough Investigation of protein structure - molecular biological methods. Growth of sequence data, cDNA, genomic sequences. Analysis of unknown DNA sequence with particular reference to protein coding regions. Prediction of protein structure and function..
3-4	4	Dr. G. Britton Investigation of protein structure - physical methods NMR, ESR, X-ray.
5-8	7	Dr. J.S. Easterby Acquisition of protein structure - folding in vitro and in vivo. Classical models of protein folding. Folding in vivo; temporal and spatial organisation of events; protein disulphide isomerase and chaperonins. Mechanisms of protein action - enzymic catalysis Transition state - stabilisation and analogues; covalent and non-covalent catalysis. Mechanism of chymotrypsin; zymogen activation; chemical, kinetic and crystallographic studies. Mechanism of lysozyme; chemical, kinetic and crystallographic studies.
8-10	6	Dr. A.D. Bates DNA-protein interactions Survey of examples of the structure of DNA-protein complexes. Parameters describing DNA conformation and application to DNA-protein complexes. Higher-order DNA-protein structures. DNA Topology DNA topology - basics and importance in vivo. DNA topoisomerases - mechanisms, drug interactions. Roles of topoisomerases in vivo - replication, transcription, recombination, cell division.
10-11	3	Dr J.S. Easterby Metabolic Control Analysis and Metabolic Pathway Dynamics Introduction to basic concepts. Flux and concentration control coefficients. Flux and concentration summation properties. Connectivity. Examples of control analysis. Genetic applications and the molecular basis of dominance. Biotechnological importance. Metabolite channelling. Temporal response of pathways. Temporal control analysis.