Super resolution techniques

Super-resolution microscopy is a fast-developing field that encompasses fluorescence imaging techniques with the capability to resolve objects below the classical diffraction limit of optical resolution.

The CCI offers several super-resolution techniques including Lattice-SIM, PALM, STORM, SRRF, Airyscan and SOFI.

Improvement in resolution from widefield to Lattice SIM. Multi-stained cells highlighting mitochondria (red), actin (green) and nucleus (blue).

Microscopes:

• Elyra (Lattice SIM, PALM, STORM)
• Andor Dragonfly spinning disk (SRRF)
• Cell Discoverer 7 (Airyscan 2)
• LSM 900 (Nuffield, Airyscan 2)
• LSM 900 (William Henry Duncan, Airyscan 2)
• LSM 880 MP (Airyscan)
• LSM 800 (Airyscan)
• Epifluorescent (SOFI)