The CCI offers FRET (fluorescence resonance energy transfer), FLIM (fluorescence lifetime imaging microscopy), FRAP (fluorescence recovery after photobleaching), and FCS (fluorescence correlation spectroscopy).
Labelled cells were imaged as a sequence of images showing movement of the cell over time. Cells attach to surfaces using protein complexes called focal adhesions. The focal adhesions of this cell were labelled with Talin-TS-GFP/RFP, a tension sensor enabling the analysis of focal adhesion force transmission and mechanosensitivity. Data courtesy of Tom Waring (Zech lab).
Microscopes:
- 3i Marianas spinning disk (FRAP, FRET)
- LSM 900 (Nuffield, FRAP)
- LSM 900 (William Henry Duncan)
- LSM 800 (FRAP)
- LSM 780 (FRAP, FRET, FLIM, FCS)
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