Spatial Profiling, also known as ‘spatially resolved transcriptomics’, was voted Nature’s method of the year in 2020 due to the versatility of the technology to address many research questions across a wide variety of areas – Oncology, Neuroscience, Infectious Disease, Inflammation and Immunity.
Spatial profiling uses a combination of immunofluorescence microscopy and next-generation sequencing to quantify gene and/or protein expression in distinct areas of your tissue.
What you'll receive from our facility
Within the facility we currently use Nanostring’s GeoMx Digital Spatial Profiler. This instrument enables users to:
- Work with FFPE, Fresh Frozen, Tissue Microarrays or slide mounted cell-culture and organoid models
- Use a standard IHC workflow to prepare slides for Spatial Profiling
- Profile multiple, user defined Regions of Interest (ROIs) across their tissue sections
- Use immunofluorescence to drive spatial profiling from distinct tissue regions (Tumour vs TME or white vs grey matter) or specific cell populations (B-cells, T-cells, macrophages, microglia etc)
- Understand Differential Gene Expression across cell types, tissue areas and across different disease states.
The equipment we offer includes:
- Nanostring’s GeoMx Digital Spatial Profiler
- Nanostring’s nCounter Pro Analysis System
- Leica Bond RXm
- HybEZ II Hybridisation System.
Who can use our facilities
- University of Liverpool academic staff
- Researchers from other universities/ research institutions
- Industrial research partners.
What Spatial Profiling can be used for
- Project consultation on spatial proteogenomic projects
- Optimisation of sample preparation and fluorescent morphology marker staining for Region of interest (ROI selection)
- Library preparation for nanostring GeoMx assays
- Sample preparation and training for nanostring nCounter Pro System.
